Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development....
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/16604 https://www.mdpi.com/2076-0817/10/11/1384 https://doi.org/10.3390/pathogens10111384 |
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| author | Bastos, Reginaldo Thekkiniath, Jose Ben Mamoun, Choukri Fuller, Lee Molestina, Robert E. Florin-Christensen, Mónica Schnittger, Leonhard Alzan, Heba F. Suarez, Carlos |
| author_browse | Alzan, Heba F. Bastos, Reginaldo Ben Mamoun, Choukri Florin-Christensen, Mónica Fuller, Lee Molestina, Robert E. Schnittger, Leonhard Suarez, Carlos Thekkiniath, Jose |
| author_facet | Bastos, Reginaldo Thekkiniath, Jose Ben Mamoun, Choukri Fuller, Lee Molestina, Robert E. Florin-Christensen, Mónica Schnittger, Leonhard Alzan, Heba F. Suarez, Carlos |
| author_sort | Bastos, Reginaldo |
| collection | INTA Digital |
| description | Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16604 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA166042024-02-14T17:28:54Z Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family Bastos, Reginaldo Thekkiniath, Jose Ben Mamoun, Choukri Fuller, Lee Molestina, Robert E. Florin-Christensen, Mónica Schnittger, Leonhard Alzan, Heba F. Suarez, Carlos Babesia microti Piroplasmosis Human Babesiosis Babesiosis Humana Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines. Instituto de Patobiología Fil: Bastos, Reginaldo. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos Fil: Thekkiniath, Jose. Fuller Laboratories; Estados Unidos Fil: Ben Mamoun, Choukri. Yale School of Medicine. Department of Internal Medicine. Section of Infectious Diseases; Estados Unidos Fil: Fuller, Lee. Fuller Laboratories; Estados Unidos Fil: Molestina, Robert E. American Type Culture Collection. Protistology Laboratory; Estados Unidos Fil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Alzan, Heba F. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos Fil: Alzan, Heba F. National Research Center. Parasitology and Animal Diseases Department; Egipto Fil: Alzan, Heba F. National Research Center. Tick and Tick-Borne Disease Research Unit; Egipto Fil: Suarez, Carlos. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos Fil: Suarez, Carlos. United States Department of Agricultural—Agricultural Research Service. Animal Disease Research Unit; Estados Unidos 2024-02-14T17:07:34Z 2024-02-14T17:07:34Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16604 https://www.mdpi.com/2076-0817/10/11/1384 2076-0817 https://doi.org/10.3390/pathogens10111384 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001, Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 10 (11) : 1384 (Noviembre 2021) |
| spellingShingle | Babesia microti Piroplasmosis Human Babesiosis Babesiosis Humana Bastos, Reginaldo Thekkiniath, Jose Ben Mamoun, Choukri Fuller, Lee Molestina, Robert E. Florin-Christensen, Mónica Schnittger, Leonhard Alzan, Heba F. Suarez, Carlos Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family |
| title | Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family |
| title_full | Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family |
| title_fullStr | Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family |
| title_full_unstemmed | Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family |
| title_short | Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family |
| title_sort | babesia microti immunoreactive rhoptry associated protein 1 paralogs are ancestral members of the piroplasmid confined rap 1 family |
| topic | Babesia microti Piroplasmosis Human Babesiosis Babesiosis Humana |
| url | http://hdl.handle.net/20.500.12123/16604 https://www.mdpi.com/2076-0817/10/11/1384 https://doi.org/10.3390/pathogens10111384 |
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