A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
The use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing th...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer Nature
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/16595 https://link.springer.com/article/10.1007/s00764-023-00273-w https://doi.org/10.1007/s00764-023-00273-w |
| _version_ | 1855485795515760640 |
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| author | Micheloni, Oscar Bernardo Ramallo, Ivana Ayelen Christeler, Bernardo Farroni, Abel Eduardo Furlan, Ricardo Luis Eugenio |
| author_browse | Christeler, Bernardo Farroni, Abel Eduardo Furlan, Ricardo Luis Eugenio Micheloni, Oscar Bernardo Ramallo, Ivana Ayelen |
| author_facet | Micheloni, Oscar Bernardo Ramallo, Ivana Ayelen Christeler, Bernardo Farroni, Abel Eduardo Furlan, Ricardo Luis Eugenio |
| author_sort | Micheloni, Oscar Bernardo |
| collection | INTA Digital |
| description | The use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing the antioxidant properties of complex natural extracts requires simple, cost-effective tools. The cupric reducing antioxidant capacity (CUPRAC) method is a reference for evaluating plant extract antioxidant capacity. A new CUPRAC thin-layer chromatography (TLC) assay, involving agarose immobilized reagents, was developed and applied to investigate the antioxidant properties of Solanum sisymbriifolium Lam., Sinapis arvensis L., and Cichorium intybus L. The best results were obtained by pouring a solution containing agarose, copper(II) chloride, 2,2-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS), and neocuproine in ammonium acetate buffer over a TLC plate at 40 °C (with final concentrations of 0.485 µmoles/cm2 copper(II) chloride and 0.368 µmoles/cm2 neocuproine) and incubating for 15 min. The limits of detection and quantification for Trolox® were 0.09 and 0.30 µg, respectively. |
| format | Artículo |
| id | INTA16595 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA165952024-02-14T14:05:53Z A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography Micheloni, Oscar Bernardo Ramallo, Ivana Ayelen Christeler, Bernardo Farroni, Abel Eduardo Furlan, Ricardo Luis Eugenio Productos de Origen Vegetal Procesamiento de Alimentos Mejoramiento Nutricional Antioxidantes Extractos Carroñeros Plant Products Food Processing Nutrient Improvement Antioxidants Extracts Scavengers Autography The use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing the antioxidant properties of complex natural extracts requires simple, cost-effective tools. The cupric reducing antioxidant capacity (CUPRAC) method is a reference for evaluating plant extract antioxidant capacity. A new CUPRAC thin-layer chromatography (TLC) assay, involving agarose immobilized reagents, was developed and applied to investigate the antioxidant properties of Solanum sisymbriifolium Lam., Sinapis arvensis L., and Cichorium intybus L. The best results were obtained by pouring a solution containing agarose, copper(II) chloride, 2,2-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS), and neocuproine in ammonium acetate buffer over a TLC plate at 40 °C (with final concentrations of 0.485 µmoles/cm2 copper(II) chloride and 0.368 µmoles/cm2 neocuproine) and incubating for 15 min. The limits of detection and quantification for Trolox® were 0.09 and 0.30 µg, respectively. EEA Pergamino Fil: Micheloni, O.B. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales. Departamento de Ciencias Básicas; Argentina Fil: Ramallo, I. A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina Fil: Ramallo, I. A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Christeler, Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; Argentina Fil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelo y Agua; Argentina Fil: Farroni, Abel Eduardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y ambientales; Argentina Fil: Furlan, Ricardo Luis Eugenio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-02-14T13:52:34Z 2024-02-14T13:52:34Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16595 https://link.springer.com/article/10.1007/s00764-023-00273-w 0933-4173 1789-0993 (online) https://doi.org/10.1007/s00764-023-00273-w eng info:eu-repograntAgreement/INTA/2023-PD-L04-I121, Diseño de alimentos funcionales y diferenciados, con énfasis en compuestos bioactivos, calidad proteica y micronutrientes info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature JPC – Journal of Planar Chromatography – Modern TLC : 1-9. (January 2024). |
| spellingShingle | Productos de Origen Vegetal Procesamiento de Alimentos Mejoramiento Nutricional Antioxidantes Extractos Carroñeros Plant Products Food Processing Nutrient Improvement Antioxidants Extracts Scavengers Autography Micheloni, Oscar Bernardo Ramallo, Ivana Ayelen Christeler, Bernardo Farroni, Abel Eduardo Furlan, Ricardo Luis Eugenio A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography |
| title | A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography |
| title_full | A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography |
| title_fullStr | A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography |
| title_full_unstemmed | A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography |
| title_short | A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography |
| title_sort | cupric reducing antioxidant capacity assay coupled to thin layer chromatography |
| topic | Productos de Origen Vegetal Procesamiento de Alimentos Mejoramiento Nutricional Antioxidantes Extractos Carroñeros Plant Products Food Processing Nutrient Improvement Antioxidants Extracts Scavengers Autography |
| url | http://hdl.handle.net/20.500.12123/16595 https://link.springer.com/article/10.1007/s00764-023-00273-w https://doi.org/10.1007/s00764-023-00273-w |
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