Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)
Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focu...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16588 https://www.mdpi.com/2072-6651/16/1/4 https://doi.org/10.3390/toxins16010004 |
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| author | Sauka, Diego Hernan Peralta, Cecilia Pérez, Melisa Paula Molla, Antonella Fernandez-Göbel, Tadeo Ocampo, Federico Palma, Leopoldo |
| author_browse | Fernandez-Göbel, Tadeo Molla, Antonella Ocampo, Federico Palma, Leopoldo Peralta, Cecilia Pérez, Melisa Paula Sauka, Diego Hernan |
| author_facet | Sauka, Diego Hernan Peralta, Cecilia Pérez, Melisa Paula Molla, Antonella Fernandez-Göbel, Tadeo Ocampo, Federico Palma, Leopoldo |
| author_sort | Sauka, Diego Hernan |
| collection | INTA Digital |
| description | Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16588 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA165882024-02-14T11:58:46Z Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) Sauka, Diego Hernan Peralta, Cecilia Pérez, Melisa Paula Molla, Antonella Fernandez-Göbel, Tadeo Ocampo, Federico Palma, Leopoldo Bacillus thuringiensis Control Biológico Plagas Biological Control Pests Insecticidal Proteins Vpb1/Vpa2 Proteins Cry8 Proteins Bioinsecticides Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Cecilia. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina Fil: Peralta, Cecilia. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; España Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Molla, Antonella. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina Fil: Fernandez-Göbel, Tadeo. Elytron Biotech S.A.; Argentina Fil: Ocampo, Federico. Elytron Biotech S.A.; Argentina Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina Fil: Palma, Leopoldo. Universitat de València. Instituto BIOTECMED. Departamento de Genética. Laboratorio de Control Biotecnológico de Plagas; España 2024-02-14T11:26:36Z 2024-02-14T11:26:36Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16588 https://www.mdpi.com/2072-6651/16/1/4 2072-6651 https://doi.org/10.3390/toxins16010004 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Toxins 16 (1) : 4 (January 2024) |
| spellingShingle | Bacillus thuringiensis Control Biológico Plagas Biological Control Pests Insecticidal Proteins Vpb1/Vpa2 Proteins Cry8 Proteins Bioinsecticides Sauka, Diego Hernan Peralta, Cecilia Pérez, Melisa Paula Molla, Antonella Fernandez-Göbel, Tadeo Ocampo, Federico Palma, Leopoldo Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) |
| title | Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) |
| title_full | Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) |
| title_fullStr | Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) |
| title_full_unstemmed | Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) |
| title_short | Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae) |
| title_sort | bacillus thuringiensis bt unvm 84 a novel strain showing insecticidal activity against anthonomus grandis boheman coleoptera curculionidae |
| topic | Bacillus thuringiensis Control Biológico Plagas Biological Control Pests Insecticidal Proteins Vpb1/Vpa2 Proteins Cry8 Proteins Bioinsecticides |
| url | http://hdl.handle.net/20.500.12123/16588 https://www.mdpi.com/2072-6651/16/1/4 https://doi.org/10.3390/toxins16010004 |
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