Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato

Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pec...

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Autores principales: Godoy, Fabiana de, Bermúdez, Luisa, Lira, Bruno Silvestre, Souza, Amanda Pereira de, Elbl, Paula, Demarco, Diego, Alseekh, Saleh, Insani, Ester Marina, Buckeridge, Marcos, Almeida, Juliana, Grigioni, Gabriela Maria, Fernie, Alisdair R., Carrari, Fernando, Rossi, Magdalena
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1655
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert106
https://doi.org/10.1093/jxb/ert106
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author Godoy, Fabiana de
Bermúdez, Luisa
Lira, Bruno Silvestre
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Ester Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author_browse Almeida, Juliana
Alseekh, Saleh
Bermúdez, Luisa
Buckeridge, Marcos
Carrari, Fernando
Demarco, Diego
Elbl, Paula
Fernie, Alisdair R.
Godoy, Fabiana de
Grigioni, Gabriela Maria
Insani, Ester Marina
Lira, Bruno Silvestre
Rossi, Magdalena
Souza, Amanda Pereira de
author_facet Godoy, Fabiana de
Bermúdez, Luisa
Lira, Bruno Silvestre
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Ester Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author_sort Godoy, Fabiana de
collection INTA Digital
description Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomato
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spelling INTA16552019-01-24T17:24:31Z Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato Godoy, Fabiana de Bermúdez, Luisa Lira, Bruno Silvestre Souza, Amanda Pereira de Elbl, Paula Demarco, Diego Alseekh, Saleh Insani, Ester Marina Buckeridge, Marcos Almeida, Juliana Grigioni, Gabriela Maria Fernie, Alisdair R. Carrari, Fernando Rossi, Magdalena Tomate Pectinas Tomatoes Pectins Carbon Carbono Galacturonosyltransferase Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomato Fil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Bermúdez, Luisa. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Souza, Amanda Pereira de. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Elbl, Paula. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Buckeridge, Marcos. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica; Brasil Fil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina Fil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica; Brasil 2017-11-02T13:04:18Z 2017-11-02T13:04:18Z 2013-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1655 https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert106 0022-0957 (Print) 1460-2431 (Online) https://doi.org/10.1093/jxb/ert106 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Experimental Botany 64 (8) : 2449–2466 (May 2013)
spellingShingle Tomate
Pectinas
Tomatoes
Pectins
Carbon
Carbono
Galacturonosyltransferase
Godoy, Fabiana de
Bermúdez, Luisa
Lira, Bruno Silvestre
Souza, Amanda Pereira de
Elbl, Paula
Demarco, Diego
Alseekh, Saleh
Insani, Ester Marina
Buckeridge, Marcos
Almeida, Juliana
Grigioni, Gabriela Maria
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_full Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_fullStr Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_full_unstemmed Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_short Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
title_sort galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato
topic Tomate
Pectinas
Tomatoes
Pectins
Carbon
Carbono
Galacturonosyltransferase
url http://hdl.handle.net/20.500.12123/1655
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert106
https://doi.org/10.1093/jxb/ert106
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