Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics
This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of field-collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888), and in geo-referenced sequenc...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2024
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16520 https://www.mdpi.com/2076-0817/12/11/1344 https://doi.org/10.3390/pathogens12111344 |
| _version_ | 1855485780078624768 |
|---|---|
| author | Díaz-Sánchez, Sandra Hernández-Triana, Luis M. Labruna, Marcelo B. Merino, Octavio Mosqueda, Juan Nava, Santiago Szabó, Matías P.J. Tarragona, Evelina Luisa Venzal, José Manuel de La Fuente, José Estrada-Peña, Agustín |
| author_browse | Díaz-Sánchez, Sandra Estrada-Peña, Agustín Hernández-Triana, Luis M. Labruna, Marcelo B. Merino, Octavio Mosqueda, Juan Nava, Santiago Szabó, Matías P.J. Tarragona, Evelina Luisa Venzal, José Manuel de La Fuente, José |
| author_facet | Díaz-Sánchez, Sandra Hernández-Triana, Luis M. Labruna, Marcelo B. Merino, Octavio Mosqueda, Juan Nava, Santiago Szabó, Matías P.J. Tarragona, Evelina Luisa Venzal, José Manuel de La Fuente, José Estrada-Peña, Agustín |
| author_sort | Díaz-Sánchez, Sandra |
| collection | INTA Digital |
| description | This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of field-collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888), and in geo-referenced sequences obtained from GenBank. Since the tick is currently considered to be a complex of cryptic taxa in several regions of the world, the main aims of the study are (i) to provide evidence of the clades of the tick present in the Neotropics, (ii) to explore if there is an effect of climate traits on the divergence rates of the target genes, and (iii) to check for a relationship between geographical and genetic distance among populations (the closest, the most similar, meaning for slow spread). We included published sequences of Rhipicephalus annulatus (Nearctic, Afrotropical, and Mediterranean) and R. microplus (Afrotropical, Indomalayan) to fully characterize the Neotropical populations (total: 74 16S, 44 COI, and 49 ITS2 sequences included in the analysis). Only the clade A of R. microplus spread in the Nearctic–Neotropics. Both the K and Lambda’s statistics, two measures of phylogenetic signal, support low divergence rates of the tested genes in populations of R. microplus in the Neotropics. These tests demonstrate that genetic diversity of the continental populations does not correlate either with the geographic distance among samples or with environmental variables. The low variability of these genes may be due to a combination of factors like (i) the recent introduction of the tick in the Neotropics, (ii) a large, effective, and fast exchange of populations, and (iii) a low effect of climate on the evolution rates of the target genes. These results have implications for the ecological studies and control of cattle tick infestations. |
| format | Artículo |
| id | INTA16520 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA165202024-01-11T11:07:29Z Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics Díaz-Sánchez, Sandra Hernández-Triana, Luis M. Labruna, Marcelo B. Merino, Octavio Mosqueda, Juan Nava, Santiago Szabó, Matías P.J. Tarragona, Evelina Luisa Venzal, José Manuel de La Fuente, José Estrada-Peña, Agustín Rhipicephalus Ganado Bovino Ácaros Nocivos Variación Genética Clado Región Neotropical Cattle Pest Mites Genetic Variation Clade Neotropical Region Rhipicephalus microplus Garrapatas Ticks This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of field-collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888), and in geo-referenced sequences obtained from GenBank. Since the tick is currently considered to be a complex of cryptic taxa in several regions of the world, the main aims of the study are (i) to provide evidence of the clades of the tick present in the Neotropics, (ii) to explore if there is an effect of climate traits on the divergence rates of the target genes, and (iii) to check for a relationship between geographical and genetic distance among populations (the closest, the most similar, meaning for slow spread). We included published sequences of Rhipicephalus annulatus (Nearctic, Afrotropical, and Mediterranean) and R. microplus (Afrotropical, Indomalayan) to fully characterize the Neotropical populations (total: 74 16S, 44 COI, and 49 ITS2 sequences included in the analysis). Only the clade A of R. microplus spread in the Nearctic–Neotropics. Both the K and Lambda’s statistics, two measures of phylogenetic signal, support low divergence rates of the tested genes in populations of R. microplus in the Neotropics. These tests demonstrate that genetic diversity of the continental populations does not correlate either with the geographic distance among samples or with environmental variables. The low variability of these genes may be due to a combination of factors like (i) the recent introduction of the tick in the Neotropics, (ii) a large, effective, and fast exchange of populations, and (iii) a low effect of climate on the evolution rates of the target genes. These results have implications for the ecological studies and control of cattle tick infestations. EEA Rafaela Fil: Díaz-Sánchez, Sandra. IREC-CSIC-UCLM-JCCM. SaBio. Instituto de Investigación en Recursos Cinegéticos; España Fil: Hernández-Triana, Luis M. Animal and Plant Health Agency. Virology Department; Reino Unido Fil: Labruna, Marcelo. Universidade de São Paulo. Faculdade de Medicina Veterinaria e Zootecnia. Departamento de Medicina Veterinaria Preventiva e Saude Animal; Brasil Fil: Merino, Octavio. Universidad Autónoma de Tamaulipas. Faculty of Veterinary Medicine; México Fil: Mosqueda, Juan. Autonomous University of Queretaro. College of Natural Sciences. Immunology and Vaccines Laboratory; México Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Szabó, Matías P.J. Universidade Federal de Uberlandia. Faculdade de Medicina Veterinaria; Brasil Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: De La Fuente, José. IREC-CSIC-UCLM-JCCM. SaBio. Instituto de Investigación en Recursos Cinegéticos; España Fil: De La Fuente, José. Oklahoma State University. Center for Veterinary Health Sciences. Department of Veterinary Pathobiology; Estados Unidos Fil: Estrada-Peña, Agustin. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España Fil: Estrada-Peña, Agustín. Instituto Agroalimentario de Aragón. Research Group in Emerging Zoonoses; España 2024-01-11T11:03:53Z 2024-01-11T11:03:53Z 2023-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16520 https://www.mdpi.com/2076-0817/12/11/1344 2076-0817 https://doi.org/10.3390/pathogens12111344 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 12 (11) : 1344 (2023) |
| spellingShingle | Rhipicephalus Ganado Bovino Ácaros Nocivos Variación Genética Clado Región Neotropical Cattle Pest Mites Genetic Variation Clade Neotropical Region Rhipicephalus microplus Garrapatas Ticks Díaz-Sánchez, Sandra Hernández-Triana, Luis M. Labruna, Marcelo B. Merino, Octavio Mosqueda, Juan Nava, Santiago Szabó, Matías P.J. Tarragona, Evelina Luisa Venzal, José Manuel de La Fuente, José Estrada-Peña, Agustín Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics |
| title | Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics |
| title_full | Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics |
| title_fullStr | Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics |
| title_full_unstemmed | Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics |
| title_short | Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics |
| title_sort | low genetic diversity of the only clade of the tick rhipicephalus microplus in the neotropics |
| topic | Rhipicephalus Ganado Bovino Ácaros Nocivos Variación Genética Clado Región Neotropical Cattle Pest Mites Genetic Variation Clade Neotropical Region Rhipicephalus microplus Garrapatas Ticks |
| url | http://hdl.handle.net/20.500.12123/16520 https://www.mdpi.com/2076-0817/12/11/1344 https://doi.org/10.3390/pathogens12111344 |
| work_keys_str_mv | AT diazsanchezsandra lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT hernandeztrianaluism lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT labrunamarcelob lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT merinooctavio lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT mosquedajuan lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT navasantiago lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT szabomatiaspj lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT tarragonaevelinaluisa lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT venzaljosemanuel lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT delafuentejose lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics AT estradapenaagustin lowgeneticdiversityoftheonlycladeofthetickrhipicephalusmicroplusintheneotropics |