Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No con...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/16510 https://www.mdpi.com/2673-6772/3/4/34 https://doi.org/10.3390/parasitologia3040034 |
| _version_ | 1855485778378883072 |
|---|---|
| author | Wieser, Sara Nathaly Decker Franco, Cecilia De Alba, Paloma Romero, Sandra Raquel Ferrari, Alejandro Schnittger, Leonhard Florin-Christensen, Monica |
| author_browse | De Alba, Paloma Decker Franco, Cecilia Ferrari, Alejandro Florin-Christensen, Monica Romero, Sandra Raquel Schnittger, Leonhard Wieser, Sara Nathaly |
| author_facet | Wieser, Sara Nathaly Decker Franco, Cecilia De Alba, Paloma Romero, Sandra Raquel Ferrari, Alejandro Schnittger, Leonhard Florin-Christensen, Monica |
| author_sort | Wieser, Sara Nathaly |
| collection | INTA Digital |
| description | Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis. |
| format | Artículo |
| id | INTA16510 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA165102024-01-10T14:59:25Z Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics Wieser, Sara Nathaly Decker Franco, Cecilia De Alba, Paloma Romero, Sandra Raquel Ferrari, Alejandro Schnittger, Leonhard Florin-Christensen, Monica Camelids Sarcocystis Sarcocystosis Llamas Alpacas Diagnostic Techniques Chemotherapeutants Antigens Camélidos Sarcocistosis Llama Alpaca Técnicas de Diagnosis Productos Quimioterapeúticos Antígenos Sarcocystis aucheniae Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis. Instituto de Patobiología Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Wieser, Sarah Nathaly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Decker Franco, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación y Desarrollo Tecnológico para la Agricultura Familiar (CIPAF). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar; Argentina Fil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ferrari, Alejandro. Instituto Nacional del Agua; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-01-10T14:35:16Z 2024-01-10T14:35:16Z 2023-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16510 https://www.mdpi.com/2673-6772/3/4/34 2673-6772 https://doi.org/10.3390/parasitologia3040034 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Parasitologia 3 (4) : 349–363 (Diciembre 2023) |
| spellingShingle | Camelids Sarcocystis Sarcocystosis Llamas Alpacas Diagnostic Techniques Chemotherapeutants Antigens Camélidos Sarcocistosis Llama Alpaca Técnicas de Diagnosis Productos Quimioterapeúticos Antígenos Sarcocystis aucheniae Wieser, Sara Nathaly Decker Franco, Cecilia De Alba, Paloma Romero, Sandra Raquel Ferrari, Alejandro Schnittger, Leonhard Florin-Christensen, Monica Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| title | Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| title_full | Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| title_fullStr | Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| title_full_unstemmed | Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| title_short | Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| title_sort | discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics |
| topic | Camelids Sarcocystis Sarcocystosis Llamas Alpacas Diagnostic Techniques Chemotherapeutants Antigens Camélidos Sarcocistosis Llama Alpaca Técnicas de Diagnosis Productos Quimioterapeúticos Antígenos Sarcocystis aucheniae |
| url | http://hdl.handle.net/20.500.12123/16510 https://www.mdpi.com/2673-6772/3/4/34 https://doi.org/10.3390/parasitologia3040034 |
| work_keys_str_mv | AT wiesersaranathaly discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics AT deckerfrancocecilia discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics AT dealbapaloma discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics AT romerosandraraquel discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics AT ferrarialejandro discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics AT schnittgerleonhard discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics AT florinchristensenmonica discoveryofantigensandcellularmechanismsintheprotozoanparasitesarcocystisaucheniaeusingimmunoproteomics |