Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai
Maternal photoperiodic effect on Trichogramma nerudai Pintureau et Gerding (Hymenoptera Trichogrammatidae) was evaluated to test its possible exploitation to store the parasitoid. Freshly parasitized eggs of Sitotroga cerealella (Olivier) (Lepidoptera Gelechiidae) were randomly assigned to one of th...
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| Format: | Artículo |
| Language: | Inglés |
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Department of Agroenvironmental Sciences and Technologies, Università di Bologna
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/16502 http://www.bulletinofinsectology.org/pdfarticles/vol76-2023-095-100viscarret.pdf |
| _version_ | 1855485776996859904 |
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| author | Viscarret, Mariana Mabel Cagnotti, Cynthia Lorena Lopez, Silvia Noemi |
| author_browse | Cagnotti, Cynthia Lorena Lopez, Silvia Noemi Viscarret, Mariana Mabel |
| author_facet | Viscarret, Mariana Mabel Cagnotti, Cynthia Lorena Lopez, Silvia Noemi |
| author_sort | Viscarret, Mariana Mabel |
| collection | INTA Digital |
| description | Maternal photoperiodic effect on Trichogramma nerudai Pintureau et Gerding (Hymenoptera Trichogrammatidae) was evaluated to test its possible exploitation to store the parasitoid. Freshly parasitized eggs of Sitotroga cerealella (Olivier) (Lepidoptera Gelechiidae) were randomly assigned to one of the following light (L)/dark (D) cycles (hours) (treatments): 3L:21D, 6L:18D, 9L:15D and 12L:12D.
This was the maternal generation (P1). Once the P1 emerged, they were exposed for 24 hours to fresh eggs of S. cerealella. The resulting parasitized eggs (F1 generation) were maintained under a moderately low temperature (11 ± 2 °C) and complete darkness. All the variables registered on the F1 were affected by light/dark cycles. A longer time of emergence was registered for treatments 3L:21D and 12L:12D (94 and 92 days, respectively). The lowest times of emergence, were for 6L:18D and 9L:15D treatments (68 and 55 days, respectively). Treatment 3L:21D (80.20 ± 2.46%) presented the highest value of emergence following for 12L:12D (69.85 ± 3.10%), 6L:18D (62.70 ± 2.87%) and finally 9L:15D (25.90 ± 3.25%). About the female proportion, treatment 3L: 21D had the highest value (0.61 ± 0.02), followed by treatment 12L:12D (0.51 ± 0.02). The female proportion was similar for treatments 9L:15D (0.41 ± 0.03), and 6L:18D (0.43 ± 0.02). The non-emerged and dissected material showed a high proportion of prepupal diapausing stage on 12L:12D treatment. However, other treatments presented a high proportion of pupal and adult non-emerged showing other quiescent stages for this parasitoid. Treatment 3L:21D on P1 generation keeping the F1 on a relative low temperature showed the best combination to obtain ca 90 days of storage, with high values of adult emergence and female parasitoid proportion. |
| format | Artículo |
| id | INTA16502 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Department of Agroenvironmental Sciences and Technologies, Università di Bologna |
| publisherStr | Department of Agroenvironmental Sciences and Technologies, Università di Bologna |
| record_format | dspace |
| spelling | INTA165022024-01-10T12:45:26Z Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai Viscarret, Mariana Mabel Cagnotti, Cynthia Lorena Lopez, Silvia Noemi Control Biológico Biological Control Trichogramma Plagas Parasitoides Parasitoids Pests Trichogramma nerudai Maternal photoperiodic effect on Trichogramma nerudai Pintureau et Gerding (Hymenoptera Trichogrammatidae) was evaluated to test its possible exploitation to store the parasitoid. Freshly parasitized eggs of Sitotroga cerealella (Olivier) (Lepidoptera Gelechiidae) were randomly assigned to one of the following light (L)/dark (D) cycles (hours) (treatments): 3L:21D, 6L:18D, 9L:15D and 12L:12D. This was the maternal generation (P1). Once the P1 emerged, they were exposed for 24 hours to fresh eggs of S. cerealella. The resulting parasitized eggs (F1 generation) were maintained under a moderately low temperature (11 ± 2 °C) and complete darkness. All the variables registered on the F1 were affected by light/dark cycles. A longer time of emergence was registered for treatments 3L:21D and 12L:12D (94 and 92 days, respectively). The lowest times of emergence, were for 6L:18D and 9L:15D treatments (68 and 55 days, respectively). Treatment 3L:21D (80.20 ± 2.46%) presented the highest value of emergence following for 12L:12D (69.85 ± 3.10%), 6L:18D (62.70 ± 2.87%) and finally 9L:15D (25.90 ± 3.25%). About the female proportion, treatment 3L: 21D had the highest value (0.61 ± 0.02), followed by treatment 12L:12D (0.51 ± 0.02). The female proportion was similar for treatments 9L:15D (0.41 ± 0.03), and 6L:18D (0.43 ± 0.02). The non-emerged and dissected material showed a high proportion of prepupal diapausing stage on 12L:12D treatment. However, other treatments presented a high proportion of pupal and adult non-emerged showing other quiescent stages for this parasitoid. Treatment 3L:21D on P1 generation keeping the F1 on a relative low temperature showed the best combination to obtain ca 90 days of storage, with high values of adult emergence and female parasitoid proportion. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Viscarret, Mariana Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Cagnotti, Cynthia Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Lopez, Silvia Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2024-01-10T12:33:29Z 2024-01-10T12:33:29Z 2023-01-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16502 http://www.bulletinofinsectology.org/pdfarticles/vol76-2023-095-100viscarret.pdf 1721-8861 2283-0332 eng info:eu-repograntAgreement/INTA/PNPV-1135033/AR./Desarrollo de herramientas para el manejo integrado de artrópodos perjudiciales info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Department of Agroenvironmental Sciences and Technologies, Università di Bologna Bulletin of Insectology 76 (1) : 95-100 (enero 2023) |
| spellingShingle | Control Biológico Biological Control Trichogramma Plagas Parasitoides Parasitoids Pests Trichogramma nerudai Viscarret, Mariana Mabel Cagnotti, Cynthia Lorena Lopez, Silvia Noemi Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai |
| title | Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai |
| title_full | Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai |
| title_fullStr | Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai |
| title_full_unstemmed | Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai |
| title_short | Evaluation of the maternal photoperiodic effect as a storage method on Trichogramma nerudai |
| title_sort | evaluation of the maternal photoperiodic effect as a storage method on trichogramma nerudai |
| topic | Control Biológico Biological Control Trichogramma Plagas Parasitoides Parasitoids Pests Trichogramma nerudai |
| url | http://hdl.handle.net/20.500.12123/16502 http://www.bulletinofinsectology.org/pdfarticles/vol76-2023-095-100viscarret.pdf |
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