In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vi...
| Main Authors: | , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1650 https://oajournals.fupress.net/index.php/pm/article/view/5520 |
| _version_ | 1855034832843702272 |
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| author | Escoriaza, Maria Georgina Sansberro, Pedro Garcia Lampasona, Sandra Claudia Gatica, Marta Elcira Bottini, Ambrosio Rubén Piccoli, Patricia Noemí |
| author_browse | Bottini, Ambrosio Rubén Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gatica, Marta Elcira Piccoli, Patricia Noemí Sansberro, Pedro |
| author_facet | Escoriaza, Maria Georgina Sansberro, Pedro Garcia Lampasona, Sandra Claudia Gatica, Marta Elcira Bottini, Ambrosio Rubén Piccoli, Patricia Noemí |
| author_sort | Escoriaza, Maria Georgina |
| collection | INTA Digital |
| description | This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1650 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA16502019-11-28T13:49:26Z In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum Escoriaza, Maria Georgina Sansberro, Pedro Garcia Lampasona, Sandra Claudia Gatica, Marta Elcira Bottini, Ambrosio Rubén Piccoli, Patricia Noemí Vitis Vinifera Vid Variedades Grapevines Varieties In Vitro Experimentation Phytoalexins Terpenoids Experimentación In Vitro Fitoalexina Terpenoidos Chardonnay Phaeoacremonium Parasiticum This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis. Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; Argentina Fil: Sansberro, Pedro. Universidad Nacional del Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas.Instituto de Botánica del Nordeste; Argentina Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; Argentina Fil: Gatica, Marta Elcira. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; Argentina Fil: Bottini, Rubén. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Piccoli, Patricia Noemí. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina 2017-11-01T15:33:12Z 2017-11-01T15:33:12Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1650 https://oajournals.fupress.net/index.php/pm/article/view/5520 0031-9465 (Print) 1593-2095 (Online) eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Phytopathologia Mediterranea 52 (2) : 289−297 (2013) |
| spellingShingle | Vitis Vinifera Vid Variedades Grapevines Varieties In Vitro Experimentation Phytoalexins Terpenoids Experimentación In Vitro Fitoalexina Terpenoidos Chardonnay Phaeoacremonium Parasiticum Escoriaza, Maria Georgina Sansberro, Pedro Garcia Lampasona, Sandra Claudia Gatica, Marta Elcira Bottini, Ambrosio Rubén Piccoli, Patricia Noemí In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum |
| title | In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum |
| title_full | In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum |
| title_fullStr | In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum |
| title_full_unstemmed | In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum |
| title_short | In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum |
| title_sort | in vitro cultures of vitis vinifera l cv chardonnay synthesize the phytoalexin nerolidol upon infection by phaeoacremonium parasiticum |
| topic | Vitis Vinifera Vid Variedades Grapevines Varieties In Vitro Experimentation Phytoalexins Terpenoids Experimentación In Vitro Fitoalexina Terpenoidos Chardonnay Phaeoacremonium Parasiticum |
| url | http://hdl.handle.net/20.500.12123/1650 https://oajournals.fupress.net/index.php/pm/article/view/5520 |
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