Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season
Among agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experi...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16474 https://www.sciencedirect.com/science/article/pii/S0929139323003475 https://doi.org/10.1016/j.apsoil.2023.105149 |
| _version_ | 1855037496964939776 |
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| author | Pin Viso, Natalia Daniela Ortiz, Jimena Maury, Mariana Frene, Juan Pablo Iocoli, Gastón Alejandro Lorenzon, Claudio Antonio Rivarola, Maximo Lisandro García, Fernando O. Gudelj, Vicente Jorge Faggioli, Valeria Soledad |
| author_browse | Faggioli, Valeria Soledad Frene, Juan Pablo García, Fernando O. Gudelj, Vicente Jorge Iocoli, Gastón Alejandro Lorenzon, Claudio Antonio Maury, Mariana Ortiz, Jimena Pin Viso, Natalia Daniela Rivarola, Maximo Lisandro |
| author_facet | Pin Viso, Natalia Daniela Ortiz, Jimena Maury, Mariana Frene, Juan Pablo Iocoli, Gastón Alejandro Lorenzon, Claudio Antonio Rivarola, Maximo Lisandro García, Fernando O. Gudelj, Vicente Jorge Faggioli, Valeria Soledad |
| author_sort | Pin Viso, Natalia Daniela |
| collection | INTA Digital |
| description | Among agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experiment in a humid climate season. We used 16S rRNA amplicon sequencing and functional prediction of marker genes involved in the N cycle at two soil depths (0–5 and 5–10 cm). Samples were taken after soybean harvest in a wheat/soybean-maize crop sequence. At both soil depths, fertilisation increased the abundance of Proteobacteria while reducing the abundance of Verrucomicrobiota. Indicator species analyses revealed that MB-A2–108 (Actinobacteriota) and Nitrososphaeraceae (Archaea) were indicators of the low fertiliser rates at 0–5 cm. Instead, Rokubacteriales (Methylomirabilota) and Nitrolancea (Chloroflexi) were indicators of low and high rates, respectively, at 5–10 cm. Biological N–fixation genes (nifA, nifV nifHD1, and nifHD2) showed an inconsistent response depending on the soil depth. Yet, the high abundance of nifA revealed the presence of N-fixing microorganisms even at high levels of N fertilisation. The predominance of genes involved in the dissimilatory (nirB and nirK) and assimilatory (nirA) reduction of nitrate was steadily found at higher fertilisation. Given that the predecessor crop was a legume that did not receive N, our results revealed the substantial legacy of long-term N inputs on soil bacterial-archaeal diversity and N-cycling genes that might have been umpired by the current humid conditions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16474 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA164742025-06-13T13:00:17Z Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season Pin Viso, Natalia Daniela Ortiz, Jimena Maury, Mariana Frene, Juan Pablo Iocoli, Gastón Alejandro Lorenzon, Claudio Antonio Rivarola, Maximo Lisandro García, Fernando O. Gudelj, Vicente Jorge Faggioli, Valeria Soledad Suelo Aplicación de Abonos Microorganismos del Suelo Soil Fertilizer Application Soil Microorganisms Soil Microbiota Mineral Fertilisation Nitrogen Cycle Fertilización Inoculantes Among agronomical practices, fertilisation greatly influences soil microbial diversity and functionality and severely disturbs the global nitrogen (N) cycle. In this study, we assessed the effect of 16 years of mineral fertilisation (NPS) on soil bacterial-archaeal communities in a no-tillage experiment in a humid climate season. We used 16S rRNA amplicon sequencing and functional prediction of marker genes involved in the N cycle at two soil depths (0–5 and 5–10 cm). Samples were taken after soybean harvest in a wheat/soybean-maize crop sequence. At both soil depths, fertilisation increased the abundance of Proteobacteria while reducing the abundance of Verrucomicrobiota. Indicator species analyses revealed that MB-A2–108 (Actinobacteriota) and Nitrososphaeraceae (Archaea) were indicators of the low fertiliser rates at 0–5 cm. Instead, Rokubacteriales (Methylomirabilota) and Nitrolancea (Chloroflexi) were indicators of low and high rates, respectively, at 5–10 cm. Biological N–fixation genes (nifA, nifV nifHD1, and nifHD2) showed an inconsistent response depending on the soil depth. Yet, the high abundance of nifA revealed the presence of N-fixing microorganisms even at high levels of N fertilisation. The predominance of genes involved in the dissimilatory (nirB and nirK) and assimilatory (nirA) reduction of nitrate was steadily found at higher fertilisation. Given that the predecessor crop was a legume that did not receive N, our results revealed the substantial legacy of long-term N inputs on soil bacterial-archaeal diversity and N-cycling genes that might have been umpired by the current humid conditions. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Iocoli, Gastón Alejandro. Universidad Nacional del Sur. Departamento de Agronomía; Argentina Fil: Lorenzón, Claudio Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: García, Fernando O. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Gudelj, Vicente Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina 2024-01-08T17:39:23Z 2024-01-08T17:39:23Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16474 https://www.sciencedirect.com/science/article/pii/S0929139323003475 0929-1393 1873-0272 https://doi.org/10.1016/j.apsoil.2023.105149 eng info:eu-repograntAgreement/INTA/PNCYO-1127033/AR./Manejo nutricional de cereales y oleaginosas para la intensificación sustentable de los sistemas productivos info:eu-repograntAgreement/INTA/PNCER-2342/AR./Diagnóstico, Reposición de nutrientes y tecnología de fertilización info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Applied Soil Ecology 193 : 105149 (January 2024) |
| spellingShingle | Suelo Aplicación de Abonos Microorganismos del Suelo Soil Fertilizer Application Soil Microorganisms Soil Microbiota Mineral Fertilisation Nitrogen Cycle Fertilización Inoculantes Pin Viso, Natalia Daniela Ortiz, Jimena Maury, Mariana Frene, Juan Pablo Iocoli, Gastón Alejandro Lorenzon, Claudio Antonio Rivarola, Maximo Lisandro García, Fernando O. Gudelj, Vicente Jorge Faggioli, Valeria Soledad Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season |
| title | Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season |
| title_full | Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season |
| title_fullStr | Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season |
| title_full_unstemmed | Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season |
| title_short | Long-term maintenance rate fertilisation increases soil bacterial-archaeal community diversity in the subsoil and N-cycling potentials in a humid crop season |
| title_sort | long term maintenance rate fertilisation increases soil bacterial archaeal community diversity in the subsoil and n cycling potentials in a humid crop season |
| topic | Suelo Aplicación de Abonos Microorganismos del Suelo Soil Fertilizer Application Soil Microorganisms Soil Microbiota Mineral Fertilisation Nitrogen Cycle Fertilización Inoculantes |
| url | http://hdl.handle.net/20.500.12123/16474 https://www.sciencedirect.com/science/article/pii/S0929139323003475 https://doi.org/10.1016/j.apsoil.2023.105149 |
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