Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas
Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae, Plusiinae) is a defoliator of soybean (Glycine max L., Fabaceae), sunflower (Helianthus annuus L., Asteraceae), alfalfa (Medicago sativa L., Fabaceae), and horticultural crops in temperate southern South America; however, there is little genetic infor...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/16429 https://onlinelibrary.wiley.com/doi/10.1111/eea.13369 https://doi.org/10.1111/eea.13369 |
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| author | Decker Franco, Cecilia San Blas, Germán Balbi, Emilia Ines Fichetti, Patricia Puebla, Andrea Fabiana Toledo, Andrea Vanesa Arneodo Larochette, Joel Demian |
| author_browse | Arneodo Larochette, Joel Demian Balbi, Emilia Ines Decker Franco, Cecilia Fichetti, Patricia Puebla, Andrea Fabiana San Blas, Germán Toledo, Andrea Vanesa |
| author_facet | Decker Franco, Cecilia San Blas, Germán Balbi, Emilia Ines Fichetti, Patricia Puebla, Andrea Fabiana Toledo, Andrea Vanesa Arneodo Larochette, Joel Demian |
| author_sort | Decker Franco, Cecilia |
| collection | INTA Digital |
| description | Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae, Plusiinae) is a defoliator of soybean (Glycine max L., Fabaceae), sunflower (Helianthus annuus L., Asteraceae), alfalfa (Medicago sativa L., Fabaceae), and horticultural crops in temperate southern South America; however, there is little genetic information available about this pest insect. Recently, concerns about this insect have increased because of its suspected resistance to Bt soybean and its spread to tropical regions. To better understand pest dynamics, and therefore implement suitable management strategies, a thorough knowledge of the genetic variability and spatial structuring of populations is required. In this paper, R. nu larvae were sampled from various crops and locations in central Argentina (where the pest is endemic) to assess diversity and population structure through mtDNA sequence analysis. In addition to the analysis of the standard cytochrome c oxidase subunit I (COI) ‘barcode’ fragment, a new set of primers was developed to explore genetic variation in the cytochrome b (Cytb) gene. COI and Cytb concatenated sequences revealed an overall high haplotype diversity (mean ± SD = 0.798 ± 0.037) and low nucleotide diversity (0.00137 ± 0.00013). The haplotype network showed a total of 19 haplotypes, separated by at most five mutational steps. A main central haplotype and four additional frequent haplotypes (accounting for 80% of the specimens examined) were distributed across populations with no obvious pattern related to host plant or geographic origin. Analysis of molecular variance further supported a weak population structure, where genetic variation within populations was the main source of total variation (ca. 96%). Pairwise comparisons showed only minor genetic differentiation among some of the populations studied. Implications of these findings in the context of R. nu control are discussed. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16429 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA164292024-01-03T10:34:44Z Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas Decker Franco, Cecilia San Blas, Germán Balbi, Emilia Ines Fichetti, Patricia Puebla, Andrea Fabiana Toledo, Andrea Vanesa Arneodo Larochette, Joel Demian Rachiplusia Plagas Tomate Soja Argentina Pests Soybeans Sunflower Tomatoes Genetic Variation Variación Genética Girasol Cytochrome b Cytochrome Oxidase I Haplotype Diversity Lucerne Lepidoptera Noctuidae Plusiinae Rachiplusia nu Alfalfa Región Pampeana Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae, Plusiinae) is a defoliator of soybean (Glycine max L., Fabaceae), sunflower (Helianthus annuus L., Asteraceae), alfalfa (Medicago sativa L., Fabaceae), and horticultural crops in temperate southern South America; however, there is little genetic information available about this pest insect. Recently, concerns about this insect have increased because of its suspected resistance to Bt soybean and its spread to tropical regions. To better understand pest dynamics, and therefore implement suitable management strategies, a thorough knowledge of the genetic variability and spatial structuring of populations is required. In this paper, R. nu larvae were sampled from various crops and locations in central Argentina (where the pest is endemic) to assess diversity and population structure through mtDNA sequence analysis. In addition to the analysis of the standard cytochrome c oxidase subunit I (COI) ‘barcode’ fragment, a new set of primers was developed to explore genetic variation in the cytochrome b (Cytb) gene. COI and Cytb concatenated sequences revealed an overall high haplotype diversity (mean ± SD = 0.798 ± 0.037) and low nucleotide diversity (0.00137 ± 0.00013). The haplotype network showed a total of 19 haplotypes, separated by at most five mutational steps. A main central haplotype and four additional frequent haplotypes (accounting for 80% of the specimens examined) were distributed across populations with no obvious pattern related to host plant or geographic origin. Analysis of molecular variance further supported a weak population structure, where genetic variation within populations was the main source of total variation (ca. 96%). Pairwise comparisons showed only minor genetic differentiation among some of the populations studied. Implications of these findings in the context of R. nu control are discussed. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: San Blas, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa (INCITAP); Argentina Fil: Balbi, Emilia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Fichetti, Patricia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Protección Vegetal. Zoología Agrícola; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Biotecnología. Unidad de Genómica; Argentina Fil: Puebla, Andrea Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Toledo, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología; Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Decker Franco, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. 2024-01-03T10:09:18Z 2024-01-03T10:09:18Z 2023-09-19 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16429 https://onlinelibrary.wiley.com/doi/10.1111/eea.13369 Entomologia Experimentalis et Applicata 171 (11) : 887-895 (Septiembre 2023) 0013-8703 1570-7458 https://doi.org/10.1111/eea.13369 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repograntAgreement/INTA/2023-PD-L01-I074, Bases ecológicas y epidemiológicas para el diseño de estrategias de manejo de plagas agrícolas y forestales info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Entomologia Experimentalis et Applicata 171 (11) : 887-895 (Septiembre 2023) |
| spellingShingle | Rachiplusia Plagas Tomate Soja Argentina Pests Soybeans Sunflower Tomatoes Genetic Variation Variación Genética Girasol Cytochrome b Cytochrome Oxidase I Haplotype Diversity Lucerne Lepidoptera Noctuidae Plusiinae Rachiplusia nu Alfalfa Región Pampeana Decker Franco, Cecilia San Blas, Germán Balbi, Emilia Ines Fichetti, Patricia Puebla, Andrea Fabiana Toledo, Andrea Vanesa Arneodo Larochette, Joel Demian Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas |
| title | Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas |
| title_full | Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas |
| title_fullStr | Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas |
| title_full_unstemmed | Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas |
| title_short | Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas |
| title_sort | genetic diversity of the polyphagous pest rachiplusia nu across crops and locations in the argentine pampas |
| topic | Rachiplusia Plagas Tomate Soja Argentina Pests Soybeans Sunflower Tomatoes Genetic Variation Variación Genética Girasol Cytochrome b Cytochrome Oxidase I Haplotype Diversity Lucerne Lepidoptera Noctuidae Plusiinae Rachiplusia nu Alfalfa Región Pampeana |
| url | http://hdl.handle.net/20.500.12123/16429 https://onlinelibrary.wiley.com/doi/10.1111/eea.13369 https://doi.org/10.1111/eea.13369 |
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