Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens
Immunosuppressive diseases cause great losses in the poultry industry, increasing the susceptibility to infections by other pathogens and promoting a suboptimal response to vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of the most important around the world. IBDV infe...
| Main Authors: | , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/16425 https://www.sciencedirect.com/science/article/pii/S003257912300648X https://doi.org/10.1016/j.psj.2023.103129 |
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| author | Jaton, Juan Marcelo Gomez, Evangelina Raquel Lucero, Maria Soledad Gravisaco, María José Pinto, Silvina Vagnozzi, Ariel Eduardo Craig, María Isabel Di Giacomo, Sebastián Berinstein, Analia Chimeno Zoth, Silvina Andrea |
| author_browse | Berinstein, Analia Chimeno Zoth, Silvina Andrea Craig, María Isabel Di Giacomo, Sebastián Gomez, Evangelina Raquel Gravisaco, María José Jaton, Juan Marcelo Lucero, Maria Soledad Pinto, Silvina Vagnozzi, Ariel Eduardo |
| author_facet | Jaton, Juan Marcelo Gomez, Evangelina Raquel Lucero, Maria Soledad Gravisaco, María José Pinto, Silvina Vagnozzi, Ariel Eduardo Craig, María Isabel Di Giacomo, Sebastián Berinstein, Analia Chimeno Zoth, Silvina Andrea |
| author_sort | Jaton, Juan Marcelo |
| collection | INTA Digital |
| description | Immunosuppressive diseases cause great losses in the poultry industry, increasing the susceptibility to infections by other pathogens and promoting a suboptimal response to vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of the most important around the world. IBDV infects immature B lymphocytes, affecting the immune status of birds and facilitating infections by other pathogens such as avian infectious bronchitis virus (IBV). Although it has been reported that the interaction between these viruses increases IBV clinical signs, there are no actual studies about the interaction between regional circulating isolates that validate this statement. In this context, the objective of our work was to evaluate the effect of the interaction between local isolates of IBDV (belonging to genogroup 4) and IBV (lineage GI-16) in chickens. Thus, specific pathogen-free chickens were orally inoculated with IBDV genogroup (G) 4 or with PBS at 5 d of age. At 14-days postinoculation (dpi) the animals were intratracheally inoculated with a GI-16 IBV or with PBS. At multiple time points, groups of birds were euthanized and different parameters such as histological damage, viral load, lymphocyte populations and specific antibodies were evaluated. The success of IBDV infection was confirmed by the severity of bursal atrophy, viral detection, and presence of anti-IBDV antibodies. In IBV-infected animals, the presence of viral genome was detected in both kidney and bursa. The coinfected animals showed higher degree of lymphocyte infiltration in kidney, higher rate of animals with IBV viral genome in bursa at 28 dpi, and a clear decrease in antibody response against IBV at 28, 35, and 40 dpi. The results indicate that the infection with the local isolate of IBDV affects the immune status of the chickens, causing major severe damage, in response to IBV infection, which could consequently severely affect the local poultry industry. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16425 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA164252024-01-02T15:11:00Z Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens Jaton, Juan Marcelo Gomez, Evangelina Raquel Lucero, Maria Soledad Gravisaco, María José Pinto, Silvina Vagnozzi, Ariel Eduardo Craig, María Isabel Di Giacomo, Sebastián Berinstein, Analia Chimeno Zoth, Silvina Andrea Infectious Bursal Disease Virus Bronchitis Specific Pathogen Free State Chickens Virus Bursitis Infecciosa Bronquitis Exención de Patógenos Específicos Pollo Coinfection Local Strain Poultry Industry Coinfección Tensión Local Industria Avícola Immunosuppressive diseases cause great losses in the poultry industry, increasing the susceptibility to infections by other pathogens and promoting a suboptimal response to vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of the most important around the world. IBDV infects immature B lymphocytes, affecting the immune status of birds and facilitating infections by other pathogens such as avian infectious bronchitis virus (IBV). Although it has been reported that the interaction between these viruses increases IBV clinical signs, there are no actual studies about the interaction between regional circulating isolates that validate this statement. In this context, the objective of our work was to evaluate the effect of the interaction between local isolates of IBDV (belonging to genogroup 4) and IBV (lineage GI-16) in chickens. Thus, specific pathogen-free chickens were orally inoculated with IBDV genogroup (G) 4 or with PBS at 5 d of age. At 14-days postinoculation (dpi) the animals were intratracheally inoculated with a GI-16 IBV or with PBS. At multiple time points, groups of birds were euthanized and different parameters such as histological damage, viral load, lymphocyte populations and specific antibodies were evaluated. The success of IBDV infection was confirmed by the severity of bursal atrophy, viral detection, and presence of anti-IBDV antibodies. In IBV-infected animals, the presence of viral genome was detected in both kidney and bursa. The coinfected animals showed higher degree of lymphocyte infiltration in kidney, higher rate of animals with IBV viral genome in bursa at 28 dpi, and a clear decrease in antibody response against IBV at 28, 35, and 40 dpi. The results indicate that the infection with the local isolate of IBDV affects the immune status of the chickens, causing major severe damage, in response to IBV infection, which could consequently severely affect the local poultry industry. Instituto de Biotecnología Fil: Jaton, Juan Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Inmunología y Vacunas Aviares; Argentina Fil: Jaton, Juan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez, Evangelina Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Inmunología y Vacunas Aviares; Argentina Fil: Gomez, Evangelina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lucero, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Inmunología y Vacunas Aviares; Argentina Fil: Lucero, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Inmunología y Vacunas Aviares; Argentina Fil: Gravisaco, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Patología; Argentina Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio Avícola; Argentina Fil: Vagnozzi, Ariel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio Avícola; Argentina Fil: Craig, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio Avícola; Argentina Fil: Di Giacomo, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berinstein, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Inmunología y Vacunas Aviares; Argentina Fil: Berinstein, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Inmunología y Vacunas Aviares; Argentina Fil: Chimeno Zoth, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-01-02T14:56:58Z 2024-01-02T14:56:58Z 2023-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16425 https://www.sciencedirect.com/science/article/pii/S003257912300648X 1525-3171 https://doi.org/10.1016/j.psj.2023.103129 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001, Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Poultry Science 102 (12) : 103129 (Diciembre 2023) |
| spellingShingle | Infectious Bursal Disease Virus Bronchitis Specific Pathogen Free State Chickens Virus Bursitis Infecciosa Bronquitis Exención de Patógenos Específicos Pollo Coinfection Local Strain Poultry Industry Coinfección Tensión Local Industria Avícola Jaton, Juan Marcelo Gomez, Evangelina Raquel Lucero, Maria Soledad Gravisaco, María José Pinto, Silvina Vagnozzi, Ariel Eduardo Craig, María Isabel Di Giacomo, Sebastián Berinstein, Analia Chimeno Zoth, Silvina Andrea Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_full | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_fullStr | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_full_unstemmed | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_short | Study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen-free chickens |
| title_sort | study of coinfection with local strains of infectious bursal disease virus and infectious bronchitis virus in specific pathogen free chickens |
| topic | Infectious Bursal Disease Virus Bronchitis Specific Pathogen Free State Chickens Virus Bursitis Infecciosa Bronquitis Exención de Patógenos Específicos Pollo Coinfection Local Strain Poultry Industry Coinfección Tensión Local Industria Avícola |
| url | http://hdl.handle.net/20.500.12123/16425 https://www.sciencedirect.com/science/article/pii/S003257912300648X https://doi.org/10.1016/j.psj.2023.103129 |
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