Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation
Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro prop...
| Autores principales: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1640 https://link.springer.com/article/10.1007%2Fs00299-015-1874-x https://doi.org/10.1007/s00299-015-1874-x |
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| author | Gimenez, Magali Diana Yañez Santos, Anahi Mara Paz, Rosalia Cristina Quiroga, Mariana Paola Marfil, Carlos Federico Conci, Vilma Cecilia Garcia Lampasona, Sandra Claudia |
| author_browse | Conci, Vilma Cecilia Garcia Lampasona, Sandra Claudia Gimenez, Magali Diana Marfil, Carlos Federico Paz, Rosalia Cristina Quiroga, Mariana Paola Yañez Santos, Anahi Mara |
| author_facet | Gimenez, Magali Diana Yañez Santos, Anahi Mara Paz, Rosalia Cristina Quiroga, Mariana Paola Marfil, Carlos Federico Conci, Vilma Cecilia Garcia Lampasona, Sandra Claudia |
| author_sort | Gimenez, Magali Diana |
| collection | INTA Digital |
| description | Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our
knowledge, this is the first report on epigenetic assessment in garlic. |
| format | info:eu-repo/semantics/article |
| id | INTA1640 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA16402019-10-17T15:32:13Z Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation Gimenez, Magali Diana Yañez Santos, Anahi Mara Paz, Rosalia Cristina Quiroga, Mariana Paola Marfil, Carlos Federico Conci, Vilma Cecilia Garcia Lampasona, Sandra Claudia Genética Ajo Variación Somaclonal Experimentación In Vitro Allium Sativum Cultivo de Meristemas Genetics Garlic Somaclonal Variation In Vitro Experimentation Meristem Culture Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic. EEA Mendoza Fil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina Fil: Yañez Santos, Anahi Mara. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina Fil: Paz, Rosalia Cristina. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina Fil: Marfil, Carlos Fedrico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina 2017-11-01T12:16:47Z 2017-11-01T12:16:47Z 2016-01 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1640 https://link.springer.com/article/10.1007%2Fs00299-015-1874-x 0721-7714 (Print) 1432-203X (Online) https://doi.org/10.1007/s00299-015-1874-x eng info:eu-repo/semantics/restrictedAccess application/pdf Plant Cell Reports 35 (1) : 129–141 (January 2016) |
| spellingShingle | Genética Ajo Variación Somaclonal Experimentación In Vitro Allium Sativum Cultivo de Meristemas Genetics Garlic Somaclonal Variation In Vitro Experimentation Meristem Culture Gimenez, Magali Diana Yañez Santos, Anahi Mara Paz, Rosalia Cristina Quiroga, Mariana Paola Marfil, Carlos Federico Conci, Vilma Cecilia Garcia Lampasona, Sandra Claudia Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation |
| title | Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation |
| title_full | Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation |
| title_fullStr | Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation |
| title_full_unstemmed | Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation |
| title_short | Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation |
| title_sort | assessment of genetic and epigenetic changes in virus free garlic allium sativum l plants obtained by meristem culture followed by in vitro propagation |
| topic | Genética Ajo Variación Somaclonal Experimentación In Vitro Allium Sativum Cultivo de Meristemas Genetics Garlic Somaclonal Variation In Vitro Experimentation Meristem Culture |
| url | http://hdl.handle.net/20.500.12123/1640 https://link.springer.com/article/10.1007%2Fs00299-015-1874-x https://doi.org/10.1007/s00299-015-1874-x |
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