Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatm...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16388 https://link.springer.com/article/10.1007/s11947-023-03226-7 https://doi.org/10.1007/s11947-023-03226-7 |
| _version_ | 1855037480001077248 |
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| author | Capetti, Caio Arnoldi Pellegrini, Vanessa Vacilotto, Milena Moreira da Silva Curvelo, Antonio Aprigio Falvo, Maurício Guimaraes, Francisco E.G. Ontañon, Ornella Mailen Campos, Eleonora Polikarpov, Igor |
| author_browse | Arnoldi Pellegrini, Vanessa Campos, Eleonora Capetti, Caio Falvo, Maurício Guimaraes, Francisco E.G. Ontañon, Ornella Mailen Polikarpov, Igor Vacilotto, Milena Moreira da Silva Curvelo, Antonio Aprigio |
| author_facet | Capetti, Caio Arnoldi Pellegrini, Vanessa Vacilotto, Milena Moreira da Silva Curvelo, Antonio Aprigio Falvo, Maurício Guimaraes, Francisco E.G. Ontañon, Ornella Mailen Campos, Eleonora Polikarpov, Igor |
| author_sort | Capetti, Caio |
| collection | INTA Digital |
| description | Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 ± 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 ± 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16388 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA163882023-12-28T16:08:17Z Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes Capetti, Caio Arnoldi Pellegrini, Vanessa Vacilotto, Milena Moreira da Silva Curvelo, Antonio Aprigio Falvo, Maurício Guimaraes, Francisco E.G. Ontañon, Ornella Mailen Campos, Eleonora Polikarpov, Igor Hydrothermal Activity Sugar Cane Bagasse Enzymes Oligosaccharides Xylans Actividad Hidrotermal Caña de Azúcar Bagazo Enzimas Oligosacáridos Xilanos Alkali Pretreatment Xylooligosaccharides Pretratamiento Alcalino Xilooligosacáridos Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 ± 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 ± 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields. Instituto de Biotecnología Fil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil Fil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil Fil: Vacilotto, Milena Moreira. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil Fil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil Fil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil Fil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil 2023-12-28T16:03:02Z 2023-12-28T16:03:02Z 2023-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16388 https://link.springer.com/article/10.1007/s11947-023-03226-7 1935-5149 https://doi.org/10.1007/s11947-023-03226-7 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Food and Bioprocess Technology : 1-13 (Published: 18 October 2023) |
| spellingShingle | Hydrothermal Activity Sugar Cane Bagasse Enzymes Oligosaccharides Xylans Actividad Hidrotermal Caña de Azúcar Bagazo Enzimas Oligosacáridos Xilanos Alkali Pretreatment Xylooligosaccharides Pretratamiento Alcalino Xilooligosacáridos Capetti, Caio Arnoldi Pellegrini, Vanessa Vacilotto, Milena Moreira da Silva Curvelo, Antonio Aprigio Falvo, Maurício Guimaraes, Francisco E.G. Ontañon, Ornella Mailen Campos, Eleonora Polikarpov, Igor Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| title | Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| title_full | Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| title_fullStr | Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| title_full_unstemmed | Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| title_short | Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| title_sort | evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes |
| topic | Hydrothermal Activity Sugar Cane Bagasse Enzymes Oligosaccharides Xylans Actividad Hidrotermal Caña de Azúcar Bagazo Enzimas Oligosacáridos Xilanos Alkali Pretreatment Xylooligosaccharides Pretratamiento Alcalino Xilooligosacáridos |
| url | http://hdl.handle.net/20.500.12123/16388 https://link.springer.com/article/10.1007/s11947-023-03226-7 https://doi.org/10.1007/s11947-023-03226-7 |
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