Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease
This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16376 https://www.mdpi.com/2076-393X/10/9/1529 https://doi.org/10.3390/vaccines10091529 |
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| author | Parreño, Gladys Viviana Bai, Muqun Liu, Fangning Jing, Jiqiang Olney, Erika Li, Guohua Wen, Ke Yang, Xingdong Castellucc, Tammy Bui Kocher, Jacob F. Zhou, Xu Yuan, Lijuan |
| author_browse | Bai, Muqun Castellucc, Tammy Bui Jing, Jiqiang Kocher, Jacob F. Li, Guohua Liu, Fangning Olney, Erika Parreño, Gladys Viviana Wen, Ke Yang, Xingdong Yuan, Lijuan Zhou, Xu |
| author_facet | Parreño, Gladys Viviana Bai, Muqun Liu, Fangning Jing, Jiqiang Olney, Erika Li, Guohua Wen, Ke Yang, Xingdong Castellucc, Tammy Bui Kocher, Jacob F. Zhou, Xu Yuan, Lijuan |
| author_sort | Parreño, Gladys Viviana |
| collection | INTA Digital |
| description | This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16376 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA163762023-12-28T10:42:59Z Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease Parreño, Gladys Viviana Bai, Muqun Liu, Fangning Jing, Jiqiang Olney, Erika Li, Guohua Wen, Ke Yang, Xingdong Castellucc, Tammy Bui Kocher, Jacob F. Zhou, Xu Yuan, Lijuan Rotavirus Probiotics Vaccine Adjuvants Gnotobiotic Animals Swine Probióticos Coadyuvantes de Vacunas Animales Notobióticos Cerdo Lactobacillus rhamnosus Infección por Rotavirus Humano Human Rotavirus Infection This preclinical study in the gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and disease evaluates the effect of probiotic Lactobacillus rhamnosus GG (LGG) as a mucosal adjuvant on the immunogenicity and cross-protective efficacy of the Lanzhou live oral trivalent (G2, G3, G4) vaccine (TLV, aka LLR3). Gn pigs were immunized with three doses of TLV with or without concurrent administration of nine doses of LGG around the time of the first dose of the TLV vaccination, and were challenged orally with the virulent heterotypic Wa G1P[8] HRV. Three doses of TLV were highly immunogenic and conferred partial protection against the heterotypic HRV infection. LGG significantly enhanced the intestinal and systemic immune responses and improved the effectiveness of protection against the heterotypic HRV challenge-induced diarrhea and virus shedding. In conclusion, we demonstrated the immune-stimulating effects of probiotic LGG as a vaccine adjuvant and generated detailed knowledge regarding the cross-reactive and type-specific antibody and effector B and T cell immune responses induced by the TLV. Due to the low cost, ease of distribution and administration, and favorable safety profiles, LGG as an adjuvant has the potential to play a critical role in improving rotavirus vaccine efficacy and making the vaccines more cost-effective. Instituto de Virología Fil: Parreño, Gladys Viviana. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Bai, Muqun. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Bai, Muqun. Lanzhou Institute of Biological Products; China Fil: Liu, Fangning. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Jing, Jiqiang. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Jing, Jiqiang. Shandong Vocational College of Animal Husbandry and Veterinary Medicine. Department of Animal Medicine; China Fil: Olney, Erika. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Olney, Erika. Midwestern University. College of Veterinary Medicine; Estados Unidos Fil: Li, Guohua. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Wen, Ke. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Yang, Xingdong. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Castellucc, Tammy Bui. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Kocher, Jacob F. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Zhou, Xu. Lanzhou Institute of Biological Products; China Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos 2023-12-28T10:38:39Z 2023-12-28T10:38:39Z 2022-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16376 https://www.mdpi.com/2076-393X/10/9/1529 2076-393X https://doi.org/10.3390/vaccines10091529 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Vaccines 10 (9) : 1529 (Septiembre 2022) |
| spellingShingle | Rotavirus Probiotics Vaccine Adjuvants Gnotobiotic Animals Swine Probióticos Coadyuvantes de Vacunas Animales Notobióticos Cerdo Lactobacillus rhamnosus Infección por Rotavirus Humano Human Rotavirus Infection Parreño, Gladys Viviana Bai, Muqun Liu, Fangning Jing, Jiqiang Olney, Erika Li, Guohua Wen, Ke Yang, Xingdong Castellucc, Tammy Bui Kocher, Jacob F. Zhou, Xu Yuan, Lijuan Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| title | Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| title_full | Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| title_fullStr | Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| title_full_unstemmed | Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| title_short | Probiotic as adjuvant significantly improves protection of the Lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| title_sort | probiotic as adjuvant significantly improves protection of the lanzhou trivalent rotavirus vaccine against heterologous challenge in a gnotobiotic pig model of human rotavirus infection and disease |
| topic | Rotavirus Probiotics Vaccine Adjuvants Gnotobiotic Animals Swine Probióticos Coadyuvantes de Vacunas Animales Notobióticos Cerdo Lactobacillus rhamnosus Infección por Rotavirus Humano Human Rotavirus Infection |
| url | http://hdl.handle.net/20.500.12123/16376 https://www.mdpi.com/2076-393X/10/9/1529 https://doi.org/10.3390/vaccines10091529 |
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