Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina
Grapevine leafroll is one of the most important viral disease affecting grapevines the world over. One of the viruses associated with this disease is Grapevine leafroll associated virus 2 (GLRaV-2), which has two remarkable properties: high genetic variability (up to date at least 6 different lineag...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1634 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3306 http://dx.doi.org/10.4454/JPP.V97I2.002 |
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| author | Lanza Volpe, Melisa Moyano, Sabrina Noé Lijavetzky, Diego Gomez Talquenca, Gonzalo |
| author_browse | Gomez Talquenca, Gonzalo Lanza Volpe, Melisa Lijavetzky, Diego Moyano, Sabrina Noé |
| author_facet | Lanza Volpe, Melisa Moyano, Sabrina Noé Lijavetzky, Diego Gomez Talquenca, Gonzalo |
| author_sort | Lanza Volpe, Melisa |
| collection | INTA Digital |
| description | Grapevine leafroll is one of the most important viral disease affecting grapevines the world over. One of the viruses associated with this disease is Grapevine leafroll associated virus 2 (GLRaV-2), which has two remarkable properties: high genetic variability (up to date at least 6 different lineages have been reported) and the unique ability among leafroll-associated viral species to be transmitted by sap inoculation to herbaceous hosts, specifically to Nicotiana spp. The presence of this virus in Mendoza (Argentina), has previously been reported but no information is available relative to the genetic variability of GLRaV-2 isolates of and their biological behavior. The molecular characterization of the CP-p19-p24 genes of the local isolates disclosed that they belong to four of the six described GLRaV-2 lineages. Using at least one isolate of each lineage, mechanical transmission to herbaceous host of the Nicotiana genus were made. Results showed a differential behavior in some combinations isolate/Nicotiana spp. and an incompatible interaction between GLRaV-2 and two new experimental hosts, N. rustica and N. tabacum cv. Samsun and White Burley. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1634 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA16342018-07-10T12:17:35Z Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina Lanza Volpe, Melisa Moyano, Sabrina Noé Lijavetzky, Diego Gomez Talquenca, Gonzalo Vid Virus de las Plantas Grapevines Plant Viruses Nicotiana Argentina Grapevine leafroll is one of the most important viral disease affecting grapevines the world over. One of the viruses associated with this disease is Grapevine leafroll associated virus 2 (GLRaV-2), which has two remarkable properties: high genetic variability (up to date at least 6 different lineages have been reported) and the unique ability among leafroll-associated viral species to be transmitted by sap inoculation to herbaceous hosts, specifically to Nicotiana spp. The presence of this virus in Mendoza (Argentina), has previously been reported but no information is available relative to the genetic variability of GLRaV-2 isolates of and their biological behavior. The molecular characterization of the CP-p19-p24 genes of the local isolates disclosed that they belong to four of the six described GLRaV-2 lineages. Using at least one isolate of each lineage, mechanical transmission to herbaceous host of the Nicotiana genus were made. Results showed a differential behavior in some combinations isolate/Nicotiana spp. and an incompatible interaction between GLRaV-2 and two new experimental hosts, N. rustica and N. tabacum cv. Samsun and White Burley. Fil: Lanza Volpe, Melisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina Fil: Moyano, Sabrina Noé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina Fil: Lijavetzky, Diego. Instituto de Biología Agrícola Mendoza-Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Genética y Genómica de Vid; Argentina Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina 2017-10-31T14:28:35Z 2017-10-31T14:28:35Z 2015-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1634 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3306 1125-4653 http://dx.doi.org/10.4454/JPP.V97I2.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of plant pathology 97 (2) : 349-355. (2015) |
| spellingShingle | Vid Virus de las Plantas Grapevines Plant Viruses Nicotiana Argentina Lanza Volpe, Melisa Moyano, Sabrina Noé Lijavetzky, Diego Gomez Talquenca, Gonzalo Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina |
| title | Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina |
| title_full | Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina |
| title_fullStr | Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina |
| title_full_unstemmed | Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina |
| title_short | Partial molecular and biological characterization of grapevine leafroll-associated virus 2 isolates from Argentina |
| title_sort | partial molecular and biological characterization of grapevine leafroll associated virus 2 isolates from argentina |
| topic | Vid Virus de las Plantas Grapevines Plant Viruses Nicotiana Argentina |
| url | http://hdl.handle.net/20.500.12123/1634 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3306 http://dx.doi.org/10.4454/JPP.V97I2.002 |
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