Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon”
In red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of anthocyanins and both qualitative and qualitative anthocyanin...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1631 http://www.actahort.org/books/1082/1082_48.htm |
| _version_ | 1855034830321876992 |
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| author | Blanco, Maria Evangelina Gomez Talquenca, Gonzalo Casassa, Luis Federico Sari, Santiago Eduardo Torres, Maria Del Rocio Ulanovsky, Silvia Matilde Fanzone, Martín Leandro |
| author_browse | Blanco, Maria Evangelina Casassa, Luis Federico Fanzone, Martín Leandro Gomez Talquenca, Gonzalo Sari, Santiago Eduardo Torres, Maria Del Rocio Ulanovsky, Silvia Matilde |
| author_facet | Blanco, Maria Evangelina Gomez Talquenca, Gonzalo Casassa, Luis Federico Sari, Santiago Eduardo Torres, Maria Del Rocio Ulanovsky, Silvia Matilde Fanzone, Martín Leandro |
| author_sort | Blanco, Maria Evangelina |
| collection | INTA Digital |
| description | In red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of
anthocyanins and both qualitative and qualitative anthocyanin composition at
harvest are crucial factors for winemaking. Anthocyanins are synthesized through the so-called phenylpropanoid pathway. Vitis vinifera L. ‘Malbec’ and ‘Cabernet
Sauvignon’ are red cultivars adapted to similar environmental conditions. Both cultivars are usually cropped to have from medium to low yields to ensure the production of premium and ultra-premium wines. Although ‘Malbec’ has significant differences in the dihydroflavonol concentration, being 10 times higher than in other red cultivars such as ‘Cabernet Sauvignon’, this increment could be partially explained by differences in the gene sequences coding for enzymes working between the naringenin chalcone and leucoanthocyanins steps. In order to investigate the dynamics of anthocyanin accumulation in grapevines, we sequenced cDNAs coding for enzymes of the anthocyanin biosynthetic pathway isolated from grape berry skin, sampled throughout ripening from two selected clones of ‘Malbec’ and ‘Cabernet Sauvignon’. Specific primers were synthesized using EST sequences (NCBI) for flavonoid 3’5’-hydroxylase, flavanone 3-hydroxylase, flavonol synthase and dihydroflavonol 4-reductase. Total RNA was isolated from berry skins and cDNA was used to study these gene sequences. Finally, fragments were cloned and sequenced, and compared between cultivars. The comparison of the deduced aminoacidic sequences against the reference data base show identities higher than 99% with the cultivar ‘Pinot Noir’ for all the enzymes, and the identified mutations do not seem to have any effect on the protein structure. When a comparison between ‘Malbec’ and ‘Cabernet Sauvignon’ sequences was performed, the identity was also higher than 99%. Our results are the basis for future investigation on the specific gene expression, and also can be used as a trigger for future assays focused on understanding the modulator effect produced by the environment on the genotypes of these worldwide cultivars. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1631 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA16312019-03-21T18:24:15Z Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” Blanco, Maria Evangelina Gomez Talquenca, Gonzalo Casassa, Luis Federico Sari, Santiago Eduardo Torres, Maria Del Rocio Ulanovsky, Silvia Matilde Fanzone, Martín Leandro Genética Secuencia Nucleotídica Vid Biosíntesis Flavonoides Genetics Nucleotide Sequence Grapevines Biosynthesis Flavonoids Malbec Cabernet Sauvignon In red wines, important sensory attributes such as color and astringency are the result of the accumulation of anthocyanins, which are located in the berry skin (and in the pulp of teinturier cultivars). Each cultivar has a unique set of anthocyanins and both qualitative and qualitative anthocyanin composition at harvest are crucial factors for winemaking. Anthocyanins are synthesized through the so-called phenylpropanoid pathway. Vitis vinifera L. ‘Malbec’ and ‘Cabernet Sauvignon’ are red cultivars adapted to similar environmental conditions. Both cultivars are usually cropped to have from medium to low yields to ensure the production of premium and ultra-premium wines. Although ‘Malbec’ has significant differences in the dihydroflavonol concentration, being 10 times higher than in other red cultivars such as ‘Cabernet Sauvignon’, this increment could be partially explained by differences in the gene sequences coding for enzymes working between the naringenin chalcone and leucoanthocyanins steps. In order to investigate the dynamics of anthocyanin accumulation in grapevines, we sequenced cDNAs coding for enzymes of the anthocyanin biosynthetic pathway isolated from grape berry skin, sampled throughout ripening from two selected clones of ‘Malbec’ and ‘Cabernet Sauvignon’. Specific primers were synthesized using EST sequences (NCBI) for flavonoid 3’5’-hydroxylase, flavanone 3-hydroxylase, flavonol synthase and dihydroflavonol 4-reductase. Total RNA was isolated from berry skins and cDNA was used to study these gene sequences. Finally, fragments were cloned and sequenced, and compared between cultivars. The comparison of the deduced aminoacidic sequences against the reference data base show identities higher than 99% with the cultivar ‘Pinot Noir’ for all the enzymes, and the identified mutations do not seem to have any effect on the protein structure. When a comparison between ‘Malbec’ and ‘Cabernet Sauvignon’ sequences was performed, the identity was also higher than 99%. Our results are the basis for future investigation on the specific gene expression, and also can be used as a trigger for future assays focused on understanding the modulator effect produced by the environment on the genotypes of these worldwide cultivars. EEA Mendoza Fil: Blanco, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina Fil: Casassa, Luis Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina Fil: Sari, Santiago Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina Fil: Torres, María del Rocío. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina Fil: Ulanovsky, Silvia Matilde. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Recursos y Mejoramiento Genético; Argentina Fil: Fanzone, Martín Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Investigación Enológica; Argentina 2017-10-31T13:45:57Z 2017-10-31T13:45:57Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1631 http://www.actahort.org/books/1082/1082_48.htm 0567-7572 DOI: 10.17660/ActaHortic.2015.1082.48 eng info:eu-repo/semantics/restrictedAccess application/pdf Acta horticulturae 1082 : 347-352. (2015) |
| spellingShingle | Genética Secuencia Nucleotídica Vid Biosíntesis Flavonoides Genetics Nucleotide Sequence Grapevines Biosynthesis Flavonoids Malbec Cabernet Sauvignon Blanco, Maria Evangelina Gomez Talquenca, Gonzalo Casassa, Luis Federico Sari, Santiago Eduardo Torres, Maria Del Rocio Ulanovsky, Silvia Matilde Fanzone, Martín Leandro Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” |
| title | Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” |
| title_full | Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” |
| title_fullStr | Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” |
| title_full_unstemmed | Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” |
| title_short | Nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in “Malbec” and “Cabernet Sauvignon” |
| title_sort | nucleotide sequence of key genes from the biosynthesis pathway of the major flavonoid in malbec and cabernet sauvignon |
| topic | Genética Secuencia Nucleotídica Vid Biosíntesis Flavonoides Genetics Nucleotide Sequence Grapevines Biosynthesis Flavonoids Malbec Cabernet Sauvignon |
| url | http://hdl.handle.net/20.500.12123/1631 http://www.actahort.org/books/1082/1082_48.htm |
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