Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies
Tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a key pest of tomato crops. Specific and environmentally friendly control strategies against this pest, such as the sterile insect technique (SIT), are under development and sexual reproduction has emerged as a key focal point...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/16169 https://onlinelibrary.wiley.com/doi/10.1111/eea.13329 https://doi.org/10.1111/eea.13329 |
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| author | Cagnotti, Cynthia Lorena Conte, Claudia Alejandra Kramar, Jacqueline Lanzavecchia, Silvia Beatriz Lopez, Silvia Noemi |
| author_browse | Cagnotti, Cynthia Lorena Conte, Claudia Alejandra Kramar, Jacqueline Lanzavecchia, Silvia Beatriz Lopez, Silvia Noemi |
| author_facet | Cagnotti, Cynthia Lorena Conte, Claudia Alejandra Kramar, Jacqueline Lanzavecchia, Silvia Beatriz Lopez, Silvia Noemi |
| author_sort | Cagnotti, Cynthia Lorena |
| collection | INTA Digital |
| description | Tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a key pest of tomato crops. Specific and environmentally friendly control strategies against this pest, such as the sterile insect technique (SIT), are under development and sexual reproduction has emerged as a key focal point to ensure its success. In the present study, we analyzed four representative T. absoluta populations from Argentina, focusing on the molecular detection of endosymbionts potentially associated with reproductive abnormalities and on determining whether parthenogenesis can occur under laboratory conditions. Larvae of T. absoluta were collected in commercial tomato plantations from La Plata and Mar del Plata (both in Buenos Aires province), Bella Vista (Corrientes province), and Yuto (Jujuy province). Tuta absoluta adults were evaluated by standard PCR for the presence of reproductive endosymbionts (Wolbachia sp., Spiroplasma sp., Arsenophonus sp., Cardinium sp., and Rickettsia sp.). For parthenogenesis assays, 20–23 virgin females from each T. absoluta geographic population were life-long isolated in Petri dishes with a tomato leaf. Fecundity, fertility, and female longevity were estimated and compared with results from mated females from each population. Our results showed the presence of a unique endosymbiont (Wolbachia sp. supergroup B, sequence type 41). Fecundity of the unmated females was significantly lower than fecundity of mated females in all T. absoluta populations. In addition, unmated females only laid infertile eggs. In conclusion, the absence of parthenogenesis in T. absoluta from representative geographic populations supports the implementation of SIT and mating disruption as effective control strategies against this pest in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16169 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA161692023-12-11T11:00:36Z Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies Cagnotti, Cynthia Lorena Conte, Claudia Alejandra Kramar, Jacqueline Lanzavecchia, Silvia Beatriz Lopez, Silvia Noemi Tuta absoluta Argentina Control Biologico Tomate Pest Control Biological Control Tomatoes Asexual Reproduction Reproducción Asexual Control de Plagas Polilla del Tomate Tomato Leafminer Tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a key pest of tomato crops. Specific and environmentally friendly control strategies against this pest, such as the sterile insect technique (SIT), are under development and sexual reproduction has emerged as a key focal point to ensure its success. In the present study, we analyzed four representative T. absoluta populations from Argentina, focusing on the molecular detection of endosymbionts potentially associated with reproductive abnormalities and on determining whether parthenogenesis can occur under laboratory conditions. Larvae of T. absoluta were collected in commercial tomato plantations from La Plata and Mar del Plata (both in Buenos Aires province), Bella Vista (Corrientes province), and Yuto (Jujuy province). Tuta absoluta adults were evaluated by standard PCR for the presence of reproductive endosymbionts (Wolbachia sp., Spiroplasma sp., Arsenophonus sp., Cardinium sp., and Rickettsia sp.). For parthenogenesis assays, 20–23 virgin females from each T. absoluta geographic population were life-long isolated in Petri dishes with a tomato leaf. Fecundity, fertility, and female longevity were estimated and compared with results from mated females from each population. Our results showed the presence of a unique endosymbiont (Wolbachia sp. supergroup B, sequence type 41). Fecundity of the unmated females was significantly lower than fecundity of mated females in all T. absoluta populations. In addition, unmated females only laid infertile eggs. In conclusion, the absence of parthenogenesis in T. absoluta from representative geographic populations supports the implementation of SIT and mating disruption as effective control strategies against this pest in Argentina. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Cagnotti, Cynthia Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Kramar, Jacqueline. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lopez, Silvia Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones Lucha Biológica; Argentina 2023-12-11T10:45:20Z 2023-12-11T10:45:20Z 2023-06-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16169 https://onlinelibrary.wiley.com/doi/10.1111/eea.13329 Entomologia Experimentalis et Applicata 171 : 681-690 (June 2023) 0013-8703 1570-7458 https://doi.org/10.1111/eea.13329 eng info:eu-repograntAgreement/INTA/2019-PE-E4-I074-001, Manejo Integrado de Plagas info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Entomologia Experimentalis et Applicata 171 : 681-690 (June 2023) |
| spellingShingle | Tuta absoluta Argentina Control Biologico Tomate Pest Control Biological Control Tomatoes Asexual Reproduction Reproducción Asexual Control de Plagas Polilla del Tomate Tomato Leafminer Cagnotti, Cynthia Lorena Conte, Claudia Alejandra Kramar, Jacqueline Lanzavecchia, Silvia Beatriz Lopez, Silvia Noemi Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies |
| title | Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies |
| title_full | Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies |
| title_fullStr | Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies |
| title_full_unstemmed | Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies |
| title_short | Molecular detection of reproductive symbionts and parthenogenesis experiments in Tuta absoluta from Argentina: facing potential for sustainable and specific pest control strategies |
| title_sort | molecular detection of reproductive symbionts and parthenogenesis experiments in tuta absoluta from argentina facing potential for sustainable and specific pest control strategies |
| topic | Tuta absoluta Argentina Control Biologico Tomate Pest Control Biological Control Tomatoes Asexual Reproduction Reproducción Asexual Control de Plagas Polilla del Tomate Tomato Leafminer |
| url | http://hdl.handle.net/20.500.12123/16169 https://onlinelibrary.wiley.com/doi/10.1111/eea.13329 https://doi.org/10.1111/eea.13329 |
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