Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts
The utilization of grape juice from low oenological value grape varieties for bioethanol production represent an alternative for diversification and value addition in viticulture. Optimizing Very High Gravity (VHG) fermentation can significantly increase ethanol productivity while reducing water and...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16144 https://www.sciencedirect.com/science/article/pii/S240584402309816X https://doi.org/10.1016/j.heliyon.2023.e22608 |
| _version_ | 1855485700509532160 |
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| author | Rojo, Cecilia Talia, Paola Mónica Lerena, María Cecilia Ponsone, María Lorena Gonzalez, Magali Lucia Rosa Becerra, Lucia Maribel Mercado, Laura Analia Martín-Arranz, Virginia Rodriguez Gomez, Francisco Arroyo Lopez, Francisco Noe Combina, Mariana |
| author_browse | Arroyo Lopez, Francisco Noe Becerra, Lucia Maribel Combina, Mariana Gonzalez, Magali Lucia Rosa Lerena, María Cecilia Martín-Arranz, Virginia Mercado, Laura Analia Ponsone, María Lorena Rodriguez Gomez, Francisco Rojo, Cecilia Talia, Paola Mónica |
| author_facet | Rojo, Cecilia Talia, Paola Mónica Lerena, María Cecilia Ponsone, María Lorena Gonzalez, Magali Lucia Rosa Becerra, Lucia Maribel Mercado, Laura Analia Martín-Arranz, Virginia Rodriguez Gomez, Francisco Arroyo Lopez, Francisco Noe Combina, Mariana |
| author_sort | Rojo, Cecilia |
| collection | INTA Digital |
| description | The utilization of grape juice from low oenological value grape varieties for bioethanol production represent an alternative for diversification and value addition in viticulture. Optimizing Very High Gravity (VHG) fermentation can significantly increase ethanol productivity while reducing water and energy consumption. In this study, the impact of different nitrogen sources on growth and fermentative performance of locally selected yeast strains was investigated. Five yeast strains of species Saccharomyces cerevisiae and Zygosaccharomyces rouxii were cultured in both synthetic culture media and natural grape juice supplemented with ammonium sulfate (NH), yeast extract (YE), Fermaid K (FERM), and urea (U) at varying concentrations. Due to the very low fermentation rate, the Z. rouxii strain was excluded from the selection. The results obtained in synthetic medium showed that nitrogen sources that promoted growth (NH and YE) had minimal effects on fermentative performance and were highly dependent on the specific yeast strain. However, the combination of urea and ammonium favored the rate of sugar consumption. When validated in natural grape juice, urea combined with ammonium (U + NH 300 + 75 mg/L) improved both growth parameters and ethanol yield. Doubling the concentration (U + NH 600 + 150 mg/L) further enhanced sugar consumption and ethanol production while reducing unwanted by-products. The combined use of urea and ammonium exhibited a synergistic effect, making it a cost-effective nitrogen supplement for VHG fermentations. |
| format | Artículo |
| id | INTA16144 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA161442023-12-06T15:18:33Z Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts Rojo, Cecilia Talia, Paola Mónica Lerena, María Cecilia Ponsone, María Lorena Gonzalez, Magali Lucia Rosa Becerra, Lucia Maribel Mercado, Laura Analia Martín-Arranz, Virginia Rodriguez Gomez, Francisco Arroyo Lopez, Francisco Noe Combina, Mariana Nitrogen Wine Yeast Growth Fermentation Ethanol Nitrógeno Levadura de Vino Crecimiento Fermentación Etánol The utilization of grape juice from low oenological value grape varieties for bioethanol production represent an alternative for diversification and value addition in viticulture. Optimizing Very High Gravity (VHG) fermentation can significantly increase ethanol productivity while reducing water and energy consumption. In this study, the impact of different nitrogen sources on growth and fermentative performance of locally selected yeast strains was investigated. Five yeast strains of species Saccharomyces cerevisiae and Zygosaccharomyces rouxii were cultured in both synthetic culture media and natural grape juice supplemented with ammonium sulfate (NH), yeast extract (YE), Fermaid K (FERM), and urea (U) at varying concentrations. Due to the very low fermentation rate, the Z. rouxii strain was excluded from the selection. The results obtained in synthetic medium showed that nitrogen sources that promoted growth (NH and YE) had minimal effects on fermentative performance and were highly dependent on the specific yeast strain. However, the combination of urea and ammonium favored the rate of sugar consumption. When validated in natural grape juice, urea combined with ammonium (U + NH 300 + 75 mg/L) improved both growth parameters and ethanol yield. Doubling the concentration (U + NH 600 + 150 mg/L) further enhanced sugar consumption and ethanol production while reducing unwanted by-products. The combined use of urea and ammonium exhibited a synergistic effect, making it a cost-effective nitrogen supplement for VHG fermentations. Instituto de Biotecnología Fil: Rojo, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lerena, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ponsone, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ponsone, María Lorena. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Gonzalez, Magali Lucia Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Becerra, Lucia Maribel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mercado, Laura Analia. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Mendoza; Argentina Fil: Martín-Arranz, Virginia. Consejo Superior de Investigaciones Científicas (CSIC). Campus Universitario Pablo de Olavide. Instituto de la Grasa; España Fil: Rodriguez Gomez, Francisco. Consejo Superior de Investigaciones Científicas (CSIC). Campus Universitario Pablo de Olavide. Instituto de la Grasa; España Fil: Arroyo Lopez, Francisco Noe. Consejo Superior de Investigaciones Científicas (CSIC). Campus Universitario Pablo de Olavide. Instituto de la Grasa; España Fil: Combina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina 2023-12-06T15:10:19Z 2023-12-06T15:10:19Z 2023-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16144 https://www.sciencedirect.com/science/article/pii/S240584402309816X 2405-8440 https://doi.org/10.1016/j.heliyon.2023.e22608 eng info:eu-repograntAgreement/INTA/2019-PE-E7-I149-001, Bioenergía generada en origen como aporte al desarrollo territorial info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Heliyon 9 (12) : e22608 (Diciembre 2023) |
| spellingShingle | Nitrogen Wine Yeast Growth Fermentation Ethanol Nitrógeno Levadura de Vino Crecimiento Fermentación Etánol Rojo, Cecilia Talia, Paola Mónica Lerena, María Cecilia Ponsone, María Lorena Gonzalez, Magali Lucia Rosa Becerra, Lucia Maribel Mercado, Laura Analia Martín-Arranz, Virginia Rodriguez Gomez, Francisco Arroyo Lopez, Francisco Noe Combina, Mariana Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| title | Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| title_full | Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| title_fullStr | Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| title_full_unstemmed | Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| title_short | Evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| title_sort | evaluation of different nitrogen sources on growth and fermentation performance for enhancing ethanol production by wine yeasts |
| topic | Nitrogen Wine Yeast Growth Fermentation Ethanol Nitrógeno Levadura de Vino Crecimiento Fermentación Etánol |
| url | http://hdl.handle.net/20.500.12123/16144 https://www.sciencedirect.com/science/article/pii/S240584402309816X https://doi.org/10.1016/j.heliyon.2023.e22608 |
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