High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana
Sugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate g...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16038 https://link.springer.com/article/10.1007/s00344-022-10894-w https://doi.org/10.1007/s00344-022-10894-w |
| _version_ | 1855037411150528512 |
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| author | Pereyra, Cintia Parola, Rodrigo Lando, Ana Paula Rodriguez, Marianela Martínez Noël, Giselle María Astrid |
| author_browse | Lando, Ana Paula Martínez Noël, Giselle María Astrid Parola, Rodrigo Pereyra, Cintia Rodriguez, Marianela |
| author_facet | Pereyra, Cintia Parola, Rodrigo Lando, Ana Paula Rodriguez, Marianela Martínez Noël, Giselle María Astrid |
| author_sort | Pereyra, Cintia |
| collection | INTA Digital |
| description | Sugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate growth in eukaryotes. However, the specific effect of different types of sugars and their concentrations on TOR signaling has not been studied in detail. Thus, we studied the activation of the root apical meristem (RAM), which is a TOR-dependent process, and TOR activity in vivo in seedlings of Arabidopsis thaliana treated with different types and concentrations of sugars. The results show that exogenous sucrose (Suc), glucose (Glc), and fructose (in a minor extent), induced RAM reactivation and TOR activity; however, at high concentration, Glc did not induce the RAM nor the kinase activity. In contrast, trehalose was not able to affect RAM length or activate TOR. High concentration of sugars, which resemble some stress conditions, inhibited TOR activity. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16038 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA160382023-11-28T12:21:48Z High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana Pereyra, Cintia Parola, Rodrigo Lando, Ana Paula Rodriguez, Marianela Martínez Noël, Giselle María Astrid Auxins Sucrose Trehalose Auxinas Estres Sucrosa Stress Trehalosa Arabidopsis thaliana TOR kinase Sugars are not only energy sources, but also central molecular signals that regulate growth, development, and response to environmental stresses. It is well recognized that sugars activate TOR kinase, the key integrator of energy availability that connects external and internal signals to regulate growth in eukaryotes. However, the specific effect of different types of sugars and their concentrations on TOR signaling has not been studied in detail. Thus, we studied the activation of the root apical meristem (RAM), which is a TOR-dependent process, and TOR activity in vivo in seedlings of Arabidopsis thaliana treated with different types and concentrations of sugars. The results show that exogenous sucrose (Suc), glucose (Glc), and fructose (in a minor extent), induced RAM reactivation and TOR activity; however, at high concentration, Glc did not induce the RAM nor the kinase activity. In contrast, trehalose was not able to affect RAM length or activate TOR. High concentration of sugars, which resemble some stress conditions, inhibited TOR activity. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Pereyra, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Parola, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina Fil: Lando, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC). Fundación para Investigaciones Biologicas Aplicada (FIBA); Argentina 2023-11-28T12:12:44Z 2023-11-28T12:12:44Z 2023-01-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16038 https://link.springer.com/article/10.1007/s00344-022-10894-w 0721-7595 1435-8107 (online) https://doi.org/10.1007/s00344-022-10894-w eng info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Journal of Plant Growth Regulation 42 (7) : 4309–4323 (July 2023) |
| spellingShingle | Auxins Sucrose Trehalose Auxinas Estres Sucrosa Stress Trehalosa Arabidopsis thaliana TOR kinase Pereyra, Cintia Parola, Rodrigo Lando, Ana Paula Rodriguez, Marianela Martínez Noël, Giselle María Astrid High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana |
| title | High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana |
| title_full | High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana |
| title_fullStr | High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana |
| title_full_unstemmed | High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana |
| title_short | High Sugar Concentration Inhibits TOR Signaling Pathway in Arabidopsis thaliana |
| title_sort | high sugar concentration inhibits tor signaling pathway in arabidopsis thaliana |
| topic | Auxins Sucrose Trehalose Auxinas Estres Sucrosa Stress Trehalosa Arabidopsis thaliana TOR kinase |
| url | http://hdl.handle.net/20.500.12123/16038 https://link.springer.com/article/10.1007/s00344-022-10894-w https://doi.org/10.1007/s00344-022-10894-w |
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