Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA)
Fasciolosis is a parasitic disease considered as emerging and neglected by the WHO. Sheep are highly susceptible to this disease, and affected flocks experience decreased productivity due to increased mortality, and the reduced quality of their products, such as wool and meat. To effectively cont...
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| Format: | Artículo |
| Language: | Inglés |
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Taylor & Francis
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/16036 https://www.tandfonline.com/doi/full/10.1080/23144599.2023.2273678 https://doi.org/10.1080/23144599.2023.2273678 |
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| author | Ahumada, María Del Rosario Godino, Agustina Guasconi, Lorena Deheza, Carla Amaranto, Marilla Pruzzo, Cesar Iván Vitulli-Moya, Gabriel Chiapello, Laura Carrizo, María Elena Barra, José Luis Cervi, Laura |
| author_browse | Ahumada, María Del Rosario Amaranto, Marilla Barra, José Luis Carrizo, María Elena Cervi, Laura Chiapello, Laura Deheza, Carla Godino, Agustina Guasconi, Lorena Pruzzo, Cesar Iván Vitulli-Moya, Gabriel |
| author_facet | Ahumada, María Del Rosario Godino, Agustina Guasconi, Lorena Deheza, Carla Amaranto, Marilla Pruzzo, Cesar Iván Vitulli-Moya, Gabriel Chiapello, Laura Carrizo, María Elena Barra, José Luis Cervi, Laura |
| author_sort | Ahumada, María Del Rosario |
| collection | INTA Digital |
| description | Fasciolosis is a parasitic disease considered as emerging and neglected by the WHO. Sheep are highly susceptible to this disease, and affected flocks experience decreased productivity due to increased mortality, and the reduced quality of their products, such as wool and meat. To effectively control this disease, reliable and early diagnosis is essential for making decisions regarding antiparasitic application and/or the removal of affected animals. Currently, the
diagnosis of F. hepatica in sheep relies on the detection of parasite eggs in faeces, a method
that becomes reliable from week 10 post-infection. Consequently, there is a need for earlier
diagnostic tools based on immune response. However, obtaining antigens for antibody detec tion has proven to be difficult and expensive. The aim of this study was to evaluate members of the Kunitz protein family of F. hepatica expressed in the form of a fusion protein in the serological diagnosis of F. hepatica in sheep. The performance of three recombinant F. hepatica Kunitz-type inhibitors (FhKT1.1, FhKT1.3, and FhKT4) was compared with a synthetic Kunitz-type peptide (sFhKT) in sera from sheep experimentally infected with F. hepatica, using an ELISA. Of these, FhKT1.1 showed the most promising diagnostic indicators, exhibiting high precision and low cross-reactivity, and thus potential for standardized produc tion. The results of our study demonstrated that the application of FhKT1.1 is a valuable tool for early-stage diagnosis of F. hepatica in sheep. Such an early diagnosis can aid in implementing timely interventions and effectively managing the disease in sheep populations |
| format | Artículo |
| id | INTA16036 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA160362023-11-29T14:13:18Z Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) Ahumada, María Del Rosario Godino, Agustina Guasconi, Lorena Deheza, Carla Amaranto, Marilla Pruzzo, Cesar Iván Vitulli-Moya, Gabriel Chiapello, Laura Carrizo, María Elena Barra, José Luis Cervi, Laura Fascioliasis Parásitos Oveja Mortalidad del Ganado Calidad Productos de Origen Animal Parasites Ewes Livestock Mortality Quality Animal Products ELISA Fasciolosis is a parasitic disease considered as emerging and neglected by the WHO. Sheep are highly susceptible to this disease, and affected flocks experience decreased productivity due to increased mortality, and the reduced quality of their products, such as wool and meat. To effectively control this disease, reliable and early diagnosis is essential for making decisions regarding antiparasitic application and/or the removal of affected animals. Currently, the diagnosis of F. hepatica in sheep relies on the detection of parasite eggs in faeces, a method that becomes reliable from week 10 post-infection. Consequently, there is a need for earlier diagnostic tools based on immune response. However, obtaining antigens for antibody detec tion has proven to be difficult and expensive. The aim of this study was to evaluate members of the Kunitz protein family of F. hepatica expressed in the form of a fusion protein in the serological diagnosis of F. hepatica in sheep. The performance of three recombinant F. hepatica Kunitz-type inhibitors (FhKT1.1, FhKT1.3, and FhKT4) was compared with a synthetic Kunitz-type peptide (sFhKT) in sera from sheep experimentally infected with F. hepatica, using an ELISA. Of these, FhKT1.1 showed the most promising diagnostic indicators, exhibiting high precision and low cross-reactivity, and thus potential for standardized produc tion. The results of our study demonstrated that the application of FhKT1.1 is a valuable tool for early-stage diagnosis of F. hepatica in sheep. Such an early diagnosis can aid in implementing timely interventions and effectively managing the disease in sheep populations EEA Manfredi Fil: Ahumada, María. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Ahumada, María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Ahumada, María. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Ahumada, María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Godino, Agustina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina Fil: Godino, Agustina. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); Argentina Fil: Guasconi, Lorena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil. Guasconi, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Deheza, Carla. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Deheza, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBIC); Argentina Fil: Amaranto, Marilla. Universidad Nacional de Córdoba. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); Argentina Fil: Pruzzo, Cesar Iván. Universidad Nacional de La Plata. Departamento de Epizootiología y Salud Pública; Argentina Fil: Pruzzo, Cesar Iván. Universidad Nacional de La Plata. Centro de Diagnósticos e Investigación Veterinaria (CEDIVE); Argentina Fil: Vitulli-Moya, Gabriel. Universidad Nacional de La Plata. Centro de Diagnósticos e Investigación Veterinaria (CEDIVE); Argentina Fil: Chiapello, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Chiapello, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Carrizo, María Elena. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina Fil: Carrizo, María Elena. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); Argentina Fil: Barra, José Luis. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica Ranwel Caputto; Argentina Fil: Barra, José Luis. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC); Argentina Fil: Cervi, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina 2023-11-28T11:42:38Z 2023-11-28T11:42:38Z 2023-11-14 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16036 https://www.tandfonline.com/doi/full/10.1080/23144599.2023.2273678 2314-4599 https://doi.org/10.1080/23144599.2023.2273678 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis International Journal of Veterinary Science and Medicine 11 (1) : 126-137 (2023) |
| spellingShingle | Fascioliasis Parásitos Oveja Mortalidad del Ganado Calidad Productos de Origen Animal Parasites Ewes Livestock Mortality Quality Animal Products ELISA Ahumada, María Del Rosario Godino, Agustina Guasconi, Lorena Deheza, Carla Amaranto, Marilla Pruzzo, Cesar Iván Vitulli-Moya, Gabriel Chiapello, Laura Carrizo, María Elena Barra, José Luis Cervi, Laura Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) |
| title | Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) |
| title_full | Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) |
| title_fullStr | Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) |
| title_full_unstemmed | Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) |
| title_short | Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA) |
| title_sort | antibody detection against kunitz type protein in fasciola hepatica experimentally infected sheep using enzyme linked immunosorbent assay elisa |
| topic | Fascioliasis Parásitos Oveja Mortalidad del Ganado Calidad Productos de Origen Animal Parasites Ewes Livestock Mortality Quality Animal Products ELISA |
| url | http://hdl.handle.net/20.500.12123/16036 https://www.tandfonline.com/doi/full/10.1080/23144599.2023.2273678 https://doi.org/10.1080/23144599.2023.2273678 |
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