Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas
Bovine alphaherpesvirus type 1 (BoAHV-1) is associated with respiratory and reproductive syndromes. Until present the immunologic mechanisms involved in BoAHV-1 abortion are partially known. We studied key elements of the innate immune response in the placentas and fetal lungs from cattle experiment...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/15937 https://www.sciencedirect.com/science/article/pii/S0378113523002663 https://doi.org/10.1016/j.vetmic.2023.109912 |
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| author | Burucúa, Mercedes María Risalde, María Cheuquepan Valenzuel, Felipe Andres Quintana, Silvina Pérez, Sandra Canton, German Jose Moore, Prando Dadin Odeon, Anselmo Carlos Agulló-Ros, Irene Scioli, Maria Valeria Barbeito, Claudio Gustavo Morrell, Eleonora Lidia Marin, Maia Solange |
| author_browse | Agulló-Ros, Irene Barbeito, Claudio Gustavo Burucúa, Mercedes María Canton, German Jose Cheuquepan Valenzuel, Felipe Andres Marin, Maia Solange Moore, Prando Dadin Morrell, Eleonora Lidia Odeon, Anselmo Carlos Pérez, Sandra Quintana, Silvina Risalde, María Scioli, Maria Valeria |
| author_facet | Burucúa, Mercedes María Risalde, María Cheuquepan Valenzuel, Felipe Andres Quintana, Silvina Pérez, Sandra Canton, German Jose Moore, Prando Dadin Odeon, Anselmo Carlos Agulló-Ros, Irene Scioli, Maria Valeria Barbeito, Claudio Gustavo Morrell, Eleonora Lidia Marin, Maia Solange |
| author_sort | Burucúa, Mercedes María |
| collection | INTA Digital |
| description | Bovine alphaherpesvirus type 1 (BoAHV-1) is associated with respiratory and reproductive syndromes. Until present the immunologic mechanisms involved in BoAHV-1 abortion are partially known. We studied key elements of the innate immune response in the placentas and fetal lungs from cattle experimentally-inoculated with BoAHV-1. These tissues were analyzed by histopathology. Furthermore, virus identification was performed by qPCR and the expression of the inflammatory cytokines such as tumor necrosis factor-alpha, interleukin 1-alpha and inflammatory mediators like inducible nitric oxide synthase and cyclooxeganse-2 was evaluated by immunohistochemistry. The viral transplacental infection was confirmed by the detection of BoAHV-1 by qPCR in the placenta and fetal organs, which revealed mild inflammatory lesions. Inducible nitric oxide synthase immunolabelling was high in the lungs of infected fetuses and placentas, as well as for tumor necrosis factor-alpha in the pulmonary parenchyma and cyclooxeganse-2 in fetal annexes. However, the expression of interleukin 1-alpha was weak in these organs. To our knowledge, this is the first study that provides strong evidence of an early immune response to BoAHV-1 infection in the conceptus. Advances in the knowledge of the complex immunological interactions at the feto-maternal unit during BoAHV-1 infection are needed to clarify the pathogenesis of abortion. |
| format | Artículo |
| id | INTA15937 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA159372025-07-17T13:02:39Z Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas Burucúa, Mercedes María Risalde, María Cheuquepan Valenzuel, Felipe Andres Quintana, Silvina Pérez, Sandra Canton, German Jose Moore, Prando Dadin Odeon, Anselmo Carlos Agulló-Ros, Irene Scioli, Maria Valeria Barbeito, Claudio Gustavo Morrell, Eleonora Lidia Marin, Maia Solange Aborto Herpes Virus Bovino Histopatología Inmunohistoquímica Inmunidad natural Pulmones Abortion Bovine Herpesvirus Bovinae Histopathology Immunohistochemistry Natural Immunity Placenta PCR Lungs Bovine alphaherpesvirus type 1 (BoAHV-1) is associated with respiratory and reproductive syndromes. Until present the immunologic mechanisms involved in BoAHV-1 abortion are partially known. We studied key elements of the innate immune response in the placentas and fetal lungs from cattle experimentally-inoculated with BoAHV-1. These tissues were analyzed by histopathology. Furthermore, virus identification was performed by qPCR and the expression of the inflammatory cytokines such as tumor necrosis factor-alpha, interleukin 1-alpha and inflammatory mediators like inducible nitric oxide synthase and cyclooxeganse-2 was evaluated by immunohistochemistry. The viral transplacental infection was confirmed by the detection of BoAHV-1 by qPCR in the placenta and fetal organs, which revealed mild inflammatory lesions. Inducible nitric oxide synthase immunolabelling was high in the lungs of infected fetuses and placentas, as well as for tumor necrosis factor-alpha in the pulmonary parenchyma and cyclooxeganse-2 in fetal annexes. However, the expression of interleukin 1-alpha was weak in these organs. To our knowledge, this is the first study that provides strong evidence of an early immune response to BoAHV-1 infection in the conceptus. Advances in the knowledge of the complex immunological interactions at the feto-maternal unit during BoAHV-1 infection are needed to clarify the pathogenesis of abortion. EEA Balcarce Fil: Burucúa, Mercedes María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Risalde, María A.Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas y Toxicología, Grupo de Investigación GISAZ, UIC Zoonosis y Enfermedades Emergentes; España. Fil: Cheuquepán, Felipe A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones de Producción, Sanidad y Ambiente; Argentina Fil: Quintana, Silvina. Instituto de Biología Molecular Aplicada; Argentina. Fil: Pérez, Sandra E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigaciones Veterinarias de Tandil; Argentina. Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Moore, Dadin Prando.Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Agulló-Ros, Irene. Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas y Toxicología, Grupo de Investigación GISAZ, UIC Zoonosis y Enfermedades Emergentes; España. Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Barbeito, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Laboratorio de Histología y Embriología Descriptiva Experimental y Comparada; Argentina. Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Marín, Maia Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. 2023-11-13T13:22:30Z 2023-11-13T13:22:30Z 2023-11-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15937 https://www.sciencedirect.com/science/article/pii/S0378113523002663 1873-2542 (online) 0378-1135 (print) https://doi.org/10.1016/j.vetmic.2023.109912 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Microbiology 287 : 109912 (December 2023) |
| spellingShingle | Aborto Herpes Virus Bovino Histopatología Inmunohistoquímica Inmunidad natural Pulmones Abortion Bovine Herpesvirus Bovinae Histopathology Immunohistochemistry Natural Immunity Placenta PCR Lungs Burucúa, Mercedes María Risalde, María Cheuquepan Valenzuel, Felipe Andres Quintana, Silvina Pérez, Sandra Canton, German Jose Moore, Prando Dadin Odeon, Anselmo Carlos Agulló-Ros, Irene Scioli, Maria Valeria Barbeito, Claudio Gustavo Morrell, Eleonora Lidia Marin, Maia Solange Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas |
| title | Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas |
| title_full | Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas |
| title_fullStr | Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas |
| title_full_unstemmed | Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas |
| title_short | Transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of COX-2, iNOS and inflammatory cytokines in fetal lungs and placentas |
| title_sort | transplacental infection by bovine alphaherpesvirus type 1 induces protein expression of cox 2 inos and inflammatory cytokines in fetal lungs and placentas |
| topic | Aborto Herpes Virus Bovino Histopatología Inmunohistoquímica Inmunidad natural Pulmones Abortion Bovine Herpesvirus Bovinae Histopathology Immunohistochemistry Natural Immunity Placenta PCR Lungs |
| url | http://hdl.handle.net/20.500.12123/15937 https://www.sciencedirect.com/science/article/pii/S0378113523002663 https://doi.org/10.1016/j.vetmic.2023.109912 |
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