Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countri...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Sociedade Brasileira de Entomologia
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15676 https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en https://doi.org/10.1590/1806-9665-RBENT-2021-0104 |
| _version_ | 1855485614172930048 |
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| author | San Blas, Germán Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann |
| author_browse | Baudino, Estela Maris Cornejo, Paula Dolibaina, Diego Rodrigo Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann Martins Casagrande, Mirna San Blas, Germán Silva Dias, Fernando Maia Specht, Alexandre |
| author_facet | San Blas, Germán Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann |
| author_sort | San Blas, Germán |
| collection | INTA Digital |
| description | Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups. |
| format | Artículo |
| id | INTA15676 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Sociedade Brasileira de Entomologia |
| publisherStr | Sociedade Brasileira de Entomologia |
| record_format | dspace |
| spelling | INTA156762024-01-03T10:27:23Z Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America San Blas, Germán Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann Agrotis Feltia Mythimna Copitarsia Neotropical Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups. EEA Anguil Fil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina Fil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; Brasil Fil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; Brasil Fil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Casagrande, Mirna Martins. Universidade Federal do Paraná; Brasil Fil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; Brasil 2023-10-25T13:01:08Z 2023-10-25T13:01:08Z 2021-12-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15676 https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en 1806-9665 0085-5626 https://doi.org/10.1590/1806-9665-RBENT-2021-0104 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Sociedade Brasileira de Entomologia Revista Brasileira de Entomologia 65 (4) : 1-14 (2021) |
| spellingShingle | Agrotis Feltia Mythimna Copitarsia Neotropical San Blas, Germán Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
| title | Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
| title_full | Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
| title_fullStr | Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
| title_full_unstemmed | Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
| title_short | Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
| title_sort | molecular characterization and phylogenetic assessment of agricultural related noctuids lepidoptera noctuidae of south america |
| topic | Agrotis Feltia Mythimna Copitarsia Neotropical |
| url | http://hdl.handle.net/20.500.12123/15676 https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en https://doi.org/10.1590/1806-9665-RBENT-2021-0104 |
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