Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia
In the fall of 2022, decreased triclabendazole (TCBZ) efficacy against F. hepatica was suspected in a sheep farm located in the Santa Cruz province, Argentinian Patagonia. Since TCBZ-resistance in F. hepatica has never been reported in this province, this study aimed to confirm potential TCBZ-resist...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15668 https://www.sciencedirect.com/science/article/pii/S2405939023000977 https://doi.org/10.1016/j.vprsr.2023.100927 |
| _version_ | 1855485612815024128 |
|---|---|
| author | Larroza, Marcela Patricia Aguilar, Marcelo Javier Soler, Paula Mora, Julio Cesar Roa, Martin Angel Cabrera, Francisco Ra Martinez Stanziola, Juan Pablo Ceballos, Laura Alvarez, Luis |
| author_browse | Aguilar, Marcelo Javier Alvarez, Luis Cabrera, Francisco Ra Ceballos, Laura Larroza, Marcela Patricia Martinez Stanziola, Juan Pablo Mora, Julio Cesar Roa, Martin Angel Soler, Paula |
| author_facet | Larroza, Marcela Patricia Aguilar, Marcelo Javier Soler, Paula Mora, Julio Cesar Roa, Martin Angel Cabrera, Francisco Ra Martinez Stanziola, Juan Pablo Ceballos, Laura Alvarez, Luis |
| author_sort | Larroza, Marcela Patricia |
| collection | INTA Digital |
| description | In the fall of 2022, decreased triclabendazole (TCBZ) efficacy against F. hepatica was suspected in a sheep farm located in the Santa Cruz province, Argentinian Patagonia. Since TCBZ-resistance in F. hepatica has never been reported in this province, this study aimed to confirm potential TCBZ-resistance in F. hepatica and to evaluate the efficacy of closantel (CLO) and nitroxinil (NTX), through faecal egg count reduction test (FECRT), and the efficacy of albendazole (ABZ) through the in vitro egg hatch test (EHT) in sheep. Sixty-eight (68) animals were selected from a herd of eighty (80) female Merino naturally infected with F. hepatica based on eggs per gram of F. hepatica (EPGFh) counts and assigned into four (4) groups (n = 17 per group): Group Control, animals did not receive anthelmintic treatment; Group TCBZ, animals were orally treated with TCBZ (12 mg/kg); Group CLO, animals were orally treated with CLO (10 mg/kg); and Group NTX, animals were subcutaneously treated with NTX (10 mg/kg). The fluke egg output was monitored on days 0 and 21 post-treatment. For the EHT, liver fluke eggs were isolated from faecal samples (approx. 50 g) collected from animals of the control group. TCBZ efficacy against liver fluke was 53.4%, confirming the presence of TCBZ-resistant isolates on the farm. CLO and NTX were highly effective (100%) for the treatment of F. hepatica on this farm. The EHT was carried out in two different laboratories, in which was observed an ABZ efficacy of 95.8 (Bariloche) and 96.5% (Tandil). These results indicate the ABZ susceptibility of this F. hepatica isolate and the inter-laboratory precision of the test. |
| format | Artículo |
| id | INTA15668 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA156682023-10-25T10:10:46Z Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia Larroza, Marcela Patricia Aguilar, Marcelo Javier Soler, Paula Mora, Julio Cesar Roa, Martin Angel Cabrera, Francisco Ra Martinez Stanziola, Juan Pablo Ceballos, Laura Alvarez, Luis Ovinos Santa Cruz (Argentina) Resistencia a los Antihelmínticos Antihelmínticos Sheep Fasciola hepatica Benzimidazoles Resistance to Anthelmintics Anthelmintics Triclabendazole Región Patagónica In the fall of 2022, decreased triclabendazole (TCBZ) efficacy against F. hepatica was suspected in a sheep farm located in the Santa Cruz province, Argentinian Patagonia. Since TCBZ-resistance in F. hepatica has never been reported in this province, this study aimed to confirm potential TCBZ-resistance in F. hepatica and to evaluate the efficacy of closantel (CLO) and nitroxinil (NTX), through faecal egg count reduction test (FECRT), and the efficacy of albendazole (ABZ) through the in vitro egg hatch test (EHT) in sheep. Sixty-eight (68) animals were selected from a herd of eighty (80) female Merino naturally infected with F. hepatica based on eggs per gram of F. hepatica (EPGFh) counts and assigned into four (4) groups (n = 17 per group): Group Control, animals did not receive anthelmintic treatment; Group TCBZ, animals were orally treated with TCBZ (12 mg/kg); Group CLO, animals were orally treated with CLO (10 mg/kg); and Group NTX, animals were subcutaneously treated with NTX (10 mg/kg). The fluke egg output was monitored on days 0 and 21 post-treatment. For the EHT, liver fluke eggs were isolated from faecal samples (approx. 50 g) collected from animals of the control group. TCBZ efficacy against liver fluke was 53.4%, confirming the presence of TCBZ-resistant isolates on the farm. CLO and NTX were highly effective (100%) for the treatment of F. hepatica on this farm. The EHT was carried out in two different laboratories, in which was observed an ABZ efficacy of 95.8 (Bariloche) and 96.5% (Tandil). These results indicate the ABZ susceptibility of this F. hepatica isolate and the inter-laboratory precision of the test. EEA Bariloche Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina Fil: Aguilar, Marcelo Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santa Cruz. Agencia de Extensión Rural San Julián; Argentina. Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina Fil: Mora, Julio Cesar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santa Cruz. Agencia de Extensión Rural Los Antiguos; Argentina Fil: Roa, Martin Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santa Cruz. Agencia de Extensión Rural Los Antiguos; Argentina Fil: Cabrera, Francisco Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area de Produccion Animal. Grupo de Sanidad Animal; Argentina Fil: Martinez Stanziola, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro Regional Patagonia Sur. Estación Experimental Agroforestal Esquel (EEAf Esquel). Campo Anexo Trevelin; Argentina Fil: Ceballos, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Alvarez, Luís. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Alvarez, Luís. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. 2023-10-25T10:08:03Z 2023-10-25T10:08:03Z 2023-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15668 https://www.sciencedirect.com/science/article/pii/S2405939023000977 2405-9390 https://doi.org/10.1016/j.vprsr.2023.100927 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I104-001, Estudios sobre generación de resistencia a los antimicrobianos y desarrollo de alternativas profilácticas y terapéuticas info:eu-repograntAgreement/INTA/2019-PE-E1-I002-001, Tecnologías sostenibles para la estabilización y mejora de la competitividad de las cadenas productivas de ovinos, caprinos y camélidos sudamericanos info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Santa Cruz .......... (province) (World, South America, Argentina) 1001522 Elsevier Veterinary Parasitology: Regional Studies and Reports 45 : 100927. (October 2023) |
| spellingShingle | Ovinos Santa Cruz (Argentina) Resistencia a los Antihelmínticos Antihelmínticos Sheep Fasciola hepatica Benzimidazoles Resistance to Anthelmintics Anthelmintics Triclabendazole Región Patagónica Larroza, Marcela Patricia Aguilar, Marcelo Javier Soler, Paula Mora, Julio Cesar Roa, Martin Angel Cabrera, Francisco Ra Martinez Stanziola, Juan Pablo Ceballos, Laura Alvarez, Luis Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia |
| title | Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia |
| title_full | Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia |
| title_fullStr | Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia |
| title_full_unstemmed | Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia |
| title_short | Triclabendazole resistance in Fasciola hepatica: First report in sheep from the Santa Cruz province, Argentinian Patagonia |
| title_sort | triclabendazole resistance in fasciola hepatica first report in sheep from the santa cruz province argentinian patagonia |
| topic | Ovinos Santa Cruz (Argentina) Resistencia a los Antihelmínticos Antihelmínticos Sheep Fasciola hepatica Benzimidazoles Resistance to Anthelmintics Anthelmintics Triclabendazole Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/15668 https://www.sciencedirect.com/science/article/pii/S2405939023000977 https://doi.org/10.1016/j.vprsr.2023.100927 |
| work_keys_str_mv | AT larrozamarcelapatricia triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT aguilarmarcelojavier triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT solerpaula triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT morajuliocesar triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT roamartinangel triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT cabrerafranciscora triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT martinezstanziolajuanpablo triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT ceballoslaura triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia AT alvarezluis triclabendazoleresistanceinfasciolahepaticafirstreportinsheepfromthesantacruzprovinceargentinianpatagonia |