Subfunctionalization probably drives the emergence of plant growth‑promoting genes
The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, en...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15664 https://link.springer.com/article/10.1007/s13199-022-00872-x https://doi.org/10.1007/s13199-022-00872-x |
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| author | Frare, Romina Alejandra Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Frare, Romina Alejandra Fussoni, Nerina Belen Gomez, Maria Cristina Mozzicafreddo, Matteo Soto, Gabriela Cynthia |
| author_facet | Frare, Romina Alejandra Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_sort | Frare, Romina Alejandra |
| collection | INTA Digital |
| description | The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence. |
| format | Artículo |
| id | INTA15664 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA156642023-10-24T17:00:25Z Subfunctionalization probably drives the emergence of plant growth‑promoting genes Frare, Romina Alejandra Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel Azospirillum brasilense Rizosfera Medicago sativa Crecimiento de Planta Rhizosphere Plant Growth Promoting Genes Genes Promotores Alfalfa The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution and function of PHB polymerases (PhaCs) from the plant growth-promoting, endophytic, and diazotrophic bacterium Azospirillum brasilense Az39. Besides the previously reported chromosomal phaC gene (phaCAz39-Chr) involved in abiotic stress adaptation, we showed that strain Az39 contains an additional copy of the phaC gene (phaCAz39-p4) in an extra-chromosomal replicon. Heterologous expression analysis demonstrated that the phaCAz39-p4 gene codifies for a functional PHB polymerase. Comparative sequence and evolutionary analyses showed an extreme level of divergence between PhaCAz39-p4 and other known PhaCs, including PhaCAz39-Chr, suggesting a divergent evolution of paralogous copies of phaC from strain Az39. Contrary to its paralogous copy, phaCAz39-p4 is specifically induced in planta and is necessary to enhance plant growth, thus empirically supporting the predicted functional divergence. Instituto de Biotecnología Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Mozzicafreddo, Matteo. Università Politecnica delle Marche. Department of Clinical and Molecular Sciences; Italia Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Fussoni, Nerina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2023-10-24T16:54:26Z 2023-10-24T16:54:26Z 2022-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15664 https://link.springer.com/article/10.1007/s13199-022-00872-x 1878-7665 https://doi.org/10.1007/s13199-022-00872-x eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Symbiosis 87 (3) : 249-253 (Julio 2022) |
| spellingShingle | Azospirillum brasilense Rizosfera Medicago sativa Crecimiento de Planta Rhizosphere Plant Growth Promoting Genes Genes Promotores Alfalfa Frare, Romina Alejandra Mozzicafreddo, Matteo Gomez, Maria Cristina Fussoni, Nerina Belen Soto, Gabriela Cynthia Ayub, Nicolás Daniel Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
| title | Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
| title_full | Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
| title_fullStr | Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
| title_full_unstemmed | Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
| title_short | Subfunctionalization probably drives the emergence of plant growth‑promoting genes |
| title_sort | subfunctionalization probably drives the emergence of plant growth promoting genes |
| topic | Azospirillum brasilense Rizosfera Medicago sativa Crecimiento de Planta Rhizosphere Plant Growth Promoting Genes Genes Promotores Alfalfa |
| url | http://hdl.handle.net/20.500.12123/15664 https://link.springer.com/article/10.1007/s13199-022-00872-x https://doi.org/10.1007/s13199-022-00872-x |
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