Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction
In this study, we identified candidate genes associated with the reproductive success of Varroa destructor in Apis mellifera brood cells at a key point in time, the 4th day after host cell capping. First-life cycle reproducing (R) and non-reproducing (NR) mites obtained by applying a semi-field rear...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15577 https://link.springer.com/article/10.1007/s13592-023-01027-7 https://doi.org/10.1007/s13592-023-01027-7 |
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| author | Muntaabski, Irina Latorre Estivalis, Jose M. Russo, Romina Maria Landi, Lucas Agra, Marcelo Nicolás Liendo, María Clara Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
| author_browse | Agra, Marcelo Nicolás Landi, Lucas Lanzavecchia, Silvia Beatriz Latorre Estivalis, Jose M. Liendo, María Clara Muntaabski, Irina Russo, Romina Maria Scannapieco, Alejandra Carla |
| author_facet | Muntaabski, Irina Latorre Estivalis, Jose M. Russo, Romina Maria Landi, Lucas Agra, Marcelo Nicolás Liendo, María Clara Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
| author_sort | Muntaabski, Irina |
| collection | INTA Digital |
| description | In this study, we identified candidate genes associated with the reproductive success of Varroa destructor in Apis mellifera brood cells at a key point in time, the 4th day after host cell capping. First-life cycle reproducing (R) and non-reproducing (NR) mites obtained by applying a semi-field rearing protocol were analyzed by RNA-Seq and qPCR. We observed a general under-expression of genes associated with metabolism and a few over-expressed genes putatively involved in signal transduction and DNA-binding transcription factor activity in R compared to NR mites. Specifically, PTCH1 and AP-1 genes, associated with embryonic segmentation and apoptosis, emerged as the best candidates for reproductive success in R mites and the early abortion of reproduction in NR mites. We also detected differentially transcribed long non-coding RNAs between R and NR mites with cis target genes annotated as regulators of replication, transcription, and translation pathways. Some of these genes were specifically associated with embryonic development and apoptosis. We discuss putative metabolic pathways associated with V. destructor reproduction to provide novel information for developing innovative and environmentally friendly mite control strategies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15577 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA155772023-10-18T13:54:42Z Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction Muntaabski, Irina Latorre Estivalis, Jose M. Russo, Romina Maria Landi, Lucas Agra, Marcelo Nicolás Liendo, María Clara Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla Varroa destructor Reproducción Dirigida Desarrollo Embrionario Relaciones Huésped Parásito Expresión Génica Secuencia de ARN Genes Candidatos Reprouction Control Embryonic Development Host Parasite Relations Gene Expression RNA Sequence Apoptosis Candidate Genes Embryogenesis Embriogénesis In this study, we identified candidate genes associated with the reproductive success of Varroa destructor in Apis mellifera brood cells at a key point in time, the 4th day after host cell capping. First-life cycle reproducing (R) and non-reproducing (NR) mites obtained by applying a semi-field rearing protocol were analyzed by RNA-Seq and qPCR. We observed a general under-expression of genes associated with metabolism and a few over-expressed genes putatively involved in signal transduction and DNA-binding transcription factor activity in R compared to NR mites. Specifically, PTCH1 and AP-1 genes, associated with embryonic segmentation and apoptosis, emerged as the best candidates for reproductive success in R mites and the early abortion of reproduction in NR mites. We also detected differentially transcribed long non-coding RNAs between R and NR mites with cis target genes annotated as regulators of replication, transcription, and translation pathways. Some of these genes were specifically associated with embryonic development and apoptosis. We discuss putative metabolic pathways associated with V. destructor reproduction to provide novel information for developing innovative and environmentally friendly mite control strategies. Instituto de Genética Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Latorre Estivalis, Jose M. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Agra, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad Integrada INTA-Universidad Nacional de Mar del Plata; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-10-18T13:38:48Z 2023-10-18T13:38:48Z 2023-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15577 https://link.springer.com/article/10.1007/s13592-023-01027-7 1297-9678 https://doi.org/10.1007/s13592-023-01027-7 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Apidologie 54 (5) : 49 (Octubre 2023) |
| spellingShingle | Varroa destructor Reproducción Dirigida Desarrollo Embrionario Relaciones Huésped Parásito Expresión Génica Secuencia de ARN Genes Candidatos Reprouction Control Embryonic Development Host Parasite Relations Gene Expression RNA Sequence Apoptosis Candidate Genes Embryogenesis Embriogénesis Muntaabski, Irina Latorre Estivalis, Jose M. Russo, Romina Maria Landi, Lucas Agra, Marcelo Nicolás Liendo, María Clara Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction |
| title | Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction |
| title_full | Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction |
| title_fullStr | Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction |
| title_full_unstemmed | Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction |
| title_short | Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction |
| title_sort | embryogenesis and apoptosis appears as key molecular pathways involved in varroa destructor reproduction |
| topic | Varroa destructor Reproducción Dirigida Desarrollo Embrionario Relaciones Huésped Parásito Expresión Génica Secuencia de ARN Genes Candidatos Reprouction Control Embryonic Development Host Parasite Relations Gene Expression RNA Sequence Apoptosis Candidate Genes Embryogenesis Embriogénesis |
| url | http://hdl.handle.net/20.500.12123/15577 https://link.springer.com/article/10.1007/s13592-023-01027-7 https://doi.org/10.1007/s13592-023-01027-7 |
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