Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress
Aspartic proteases are proteolytic enzymes widely distributed in living organisms and viruses. Although they have been extensively studied in many plant species, they are poorly described in potatoes. The present study aimed to identify and characterize S. tuberosum aspartic proteases. Gene structur...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Multidisciplinary Digital Publishing Institute, MDPI
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/15532 https://www.mdpi.com/2223-7747/11/4/544 https://doi.org/10.3390/plants11040544 |
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| author | Norero, Natalia Sigrid Rey Burusco, María Florencia D’Ippólito, Sebastián Decima Oneto, Cecilia Andrea Massa, Gabriela Alejandra Castellote, Martín Alfredo Feingold, Sergio Enrique Guevara, María Gabriela |
| author_browse | Castellote, Martín Alfredo Decima Oneto, Cecilia Andrea D’Ippólito, Sebastián Feingold, Sergio Enrique Guevara, María Gabriela Massa, Gabriela Alejandra Norero, Natalia Sigrid Rey Burusco, María Florencia |
| author_facet | Norero, Natalia Sigrid Rey Burusco, María Florencia D’Ippólito, Sebastián Decima Oneto, Cecilia Andrea Massa, Gabriela Alejandra Castellote, Martín Alfredo Feingold, Sergio Enrique Guevara, María Gabriela |
| author_sort | Norero, Natalia Sigrid |
| collection | INTA Digital |
| description | Aspartic proteases are proteolytic enzymes widely distributed in living organisms and viruses. Although they have been extensively studied in many plant species, they are poorly described in potatoes. The present study aimed to identify and characterize S. tuberosum aspartic proteases. Gene structure, chromosome and protein domain organization, phylogeny, and subcellular predicted localization were analyzed and integrated with RNAseq data from different tissues, organs, and conditions focused on abiotic stress. Sixty-two aspartic protease genes were retrieved from the potato genome, distributed in 12 chromosomes. A high number of intronless genes and segmental and tandem duplications were detected. Phylogenetic analysis revealed eight StAP groups, named from StAPI to StAPVIII, that were differentiated into typical (StAPI), nucellin-like (StAPIIIa), and atypical aspartic proteases (StAPII, StAPIIIb to StAPVIII). RNAseq data analyses showed that gene expression was consistent with the presence of cis-acting regulatory elements on StAP promoter regions related to water deficit. The study presents the first identification and characterization of 62 aspartic protease genes and proteins on the potato genome and provides the baseline material for functional gene determinations and potato breeding programs, including gene editing mediated by CRISPR. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15532 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute, MDPI |
| publisherStr | Multidisciplinary Digital Publishing Institute, MDPI |
| record_format | dspace |
| spelling | INTA155322023-10-12T11:14:41Z Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress Norero, Natalia Sigrid Rey Burusco, María Florencia D’Ippólito, Sebastián Decima Oneto, Cecilia Andrea Massa, Gabriela Alejandra Castellote, Martín Alfredo Feingold, Sergio Enrique Guevara, María Gabriela Papa Solanum tuberosum Genomas Proteasas Estrés Abiótico Potatoes Genomes Proteases Abiotic Stress Aspartic proteases are proteolytic enzymes widely distributed in living organisms and viruses. Although they have been extensively studied in many plant species, they are poorly described in potatoes. The present study aimed to identify and characterize S. tuberosum aspartic proteases. Gene structure, chromosome and protein domain organization, phylogeny, and subcellular predicted localization were analyzed and integrated with RNAseq data from different tissues, organs, and conditions focused on abiotic stress. Sixty-two aspartic protease genes were retrieved from the potato genome, distributed in 12 chromosomes. A high number of intronless genes and segmental and tandem duplications were detected. Phylogenetic analysis revealed eight StAP groups, named from StAPI to StAPVIII, that were differentiated into typical (StAPI), nucellin-like (StAPIIIa), and atypical aspartic proteases (StAPII, StAPIIIb to StAPVIII). RNAseq data analyses showed that gene expression was consistent with the presence of cis-acting regulatory elements on StAP promoter regions related to water deficit. The study presents the first identification and characterization of 62 aspartic protease genes and proteins on the potato genome and provides the baseline material for functional gene determinations and potato breeding programs, including gene editing mediated by CRISPR. EEA Balcarce Fil: Norero, Natalia Sigrid. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Rey Burusco, María Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: D’Ippólito, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina. Instituto de Investigaciones Biológicas; Argentina. Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Massa, Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Massa, Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Castellote, Martín Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Guevara, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina. Instituto de Investigaciones Biológicas; Argentina. 2023-10-12T11:10:51Z 2023-10-12T11:10:51Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15532 https://www.mdpi.com/2223-7747/11/4/544 2223-7747 https://doi.org/10.3390/plants11040544 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001, Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Multidisciplinary Digital Publishing Institute, MDPI Plants 11 (4) : 544 (February 2022) |
| spellingShingle | Papa Solanum tuberosum Genomas Proteasas Estrés Abiótico Potatoes Genomes Proteases Abiotic Stress Norero, Natalia Sigrid Rey Burusco, María Florencia D’Ippólito, Sebastián Decima Oneto, Cecilia Andrea Massa, Gabriela Alejandra Castellote, Martín Alfredo Feingold, Sergio Enrique Guevara, María Gabriela Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress |
| title | Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress |
| title_full | Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress |
| title_fullStr | Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress |
| title_full_unstemmed | Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress |
| title_short | Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress |
| title_sort | genome wide analyses of aspartic proteases on potato genome solanum tuberosum generating new tools to improve the resistance of plants to abiotic stress |
| topic | Papa Solanum tuberosum Genomas Proteasas Estrés Abiótico Potatoes Genomes Proteases Abiotic Stress |
| url | http://hdl.handle.net/20.500.12123/15532 https://www.mdpi.com/2223-7747/11/4/544 https://doi.org/10.3390/plants11040544 |
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