PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender
Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial insemination programs. The aim of this study was to investigate the effects...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15531 https://www.sciencedirect.com/science/article/pii/S0093691X22001248 https://doi.org/10.1016/j.theriogenology.2022.03.035 |
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| author | Carro, María De Las Mercedes Luquez, J.M. Peñalva, D.A. Buschiazzo, Jorgelina Hozbor, Federico Andres Furland, N.E. |
| author_browse | Buschiazzo, Jorgelina Carro, María De Las Mercedes Furland, N.E. Hozbor, Federico Andres Luquez, J.M. Peñalva, D.A. |
| author_facet | Carro, María De Las Mercedes Luquez, J.M. Peñalva, D.A. Buschiazzo, Jorgelina Hozbor, Federico Andres Furland, N.E. |
| author_sort | Carro, María De Las Mercedes |
| collection | INTA Digital |
| description | Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial insemination programs. The aim of this study was to investigate the effects of cryopreservation with a chemically defined soybean lecithin-based extender on ram spermatozoa functionality on the one hand, and quantifiable changes in lipid and fatty acid profile on the other. Freeze-thawing decreased sperm quality, as indicated by post-thaw parameters related to membrane integrity, mitochondrial viability and sperm motility. The most relevant lipid change after cryopreservation was a remarkable loss of all glycerophospholipids containing 22:6n-3. Species of sphingomyelin with very long chain polyunsaturated fatty acids (VLC-PUFA), that are exclusively located in the sperm head, where responsible of its reduction after cryostorage. Freezing caused a reduction in mitochondrial function, which was confirmed by significantly decreased of mitochondrial membrane potential and by the generation of 4-HNE. Mitochondria damage was accompanied by a loss in cardiolipin with 18:2n-6 and phosphatidylethanolamine with 20:4n-6, two well-known lipids that are critical components for mitochondrial membrane functionality. Loss of sterols after cryopreservation occurred along with a decrease in the order of sperm membrane lipids. Our research provides new insights on deleterious effects of cryopreservation on PUFA-rich phospholipids of ram sperm and highlight their importance as biomarkers of ultrastructural, biochemical and functional damage that ram spermatozoa undergo after freezing-thawing. |
| format | Artículo |
| id | INTA15531 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA155312023-10-12T11:07:57Z PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender Carro, María De Las Mercedes Luquez, J.M. Peñalva, D.A. Buschiazzo, Jorgelina Hozbor, Federico Andres Furland, N.E. Ovinos Criopreservación Espermatozoo Fosfolípidos Ácidos Grasos Sheep Cryopreservation Spermatozoa Phospholipids Fatty Acids Carnero Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial insemination programs. The aim of this study was to investigate the effects of cryopreservation with a chemically defined soybean lecithin-based extender on ram spermatozoa functionality on the one hand, and quantifiable changes in lipid and fatty acid profile on the other. Freeze-thawing decreased sperm quality, as indicated by post-thaw parameters related to membrane integrity, mitochondrial viability and sperm motility. The most relevant lipid change after cryopreservation was a remarkable loss of all glycerophospholipids containing 22:6n-3. Species of sphingomyelin with very long chain polyunsaturated fatty acids (VLC-PUFA), that are exclusively located in the sperm head, where responsible of its reduction after cryostorage. Freezing caused a reduction in mitochondrial function, which was confirmed by significantly decreased of mitochondrial membrane potential and by the generation of 4-HNE. Mitochondria damage was accompanied by a loss in cardiolipin with 18:2n-6 and phosphatidylethanolamine with 20:4n-6, two well-known lipids that are critical components for mitochondrial membrane functionality. Loss of sterols after cryopreservation occurred along with a decrease in the order of sperm membrane lipids. Our research provides new insights on deleterious effects of cryopreservation on PUFA-rich phospholipids of ram sperm and highlight their importance as biomarkers of ultrastructural, biochemical and functional damage that ram spermatozoa undergo after freezing-thawing. Fil: Carro, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Luquez, J.M. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Fil: Peñalva, D.A. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Fil: Buschiazzo, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Hozbor, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Furland, N.E. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. 2023-10-12T11:02:32Z 2023-10-12T11:02:32Z 2022-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15531 https://www.sciencedirect.com/science/article/pii/S0093691X22001248 0093-691X (print) 1879-3231 (online) https://doi.org/10.1016/j.theriogenology.2022.03.035 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Theriogenology 186 : 122-134 (July 2022) |
| spellingShingle | Ovinos Criopreservación Espermatozoo Fosfolípidos Ácidos Grasos Sheep Cryopreservation Spermatozoa Phospholipids Fatty Acids Carnero Carro, María De Las Mercedes Luquez, J.M. Peñalva, D.A. Buschiazzo, Jorgelina Hozbor, Federico Andres Furland, N.E. PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender |
| title | PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender |
| title_full | PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender |
| title_fullStr | PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender |
| title_full_unstemmed | PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender |
| title_short | PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender |
| title_sort | pufa rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin based extender |
| topic | Ovinos Criopreservación Espermatozoo Fosfolípidos Ácidos Grasos Sheep Cryopreservation Spermatozoa Phospholipids Fatty Acids Carnero |
| url | http://hdl.handle.net/20.500.12123/15531 https://www.sciencedirect.com/science/article/pii/S0093691X22001248 https://doi.org/10.1016/j.theriogenology.2022.03.035 |
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