Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation
Gumboro illness is caused by the highly contagious immunosuppressive infectious bursal disease virus (IBDV), which affects the poultry industry globally. We have previously shown that IBDV hijacks the endocytic pathway to construct viral replication complexes on endosomes linked to the Golgi complex...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2023
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/15525 https://www.mdpi.com/1999-4915/15/6/1295 https://doi.org/10.3390/v15061295 |
| _version_ | 1855485592126619648 |
|---|---|
| author | Frontini-López, Yesica R. Rivera, Lautaro Pocognoni, Cristian A. Roldán, Julieta Suyai Colombo, Maria Isabel Uhart, Marina Delgui, Laura Ruth |
| author_browse | Colombo, Maria Isabel Delgui, Laura Ruth Frontini-López, Yesica R. Pocognoni, Cristian A. Rivera, Lautaro Roldán, Julieta Suyai Uhart, Marina |
| author_facet | Frontini-López, Yesica R. Rivera, Lautaro Pocognoni, Cristian A. Roldán, Julieta Suyai Colombo, Maria Isabel Uhart, Marina Delgui, Laura Ruth |
| author_sort | Frontini-López, Yesica R. |
| collection | INTA Digital |
| description | Gumboro illness is caused by the highly contagious immunosuppressive infectious bursal disease virus (IBDV), which affects the poultry industry globally. We have previously shown that IBDV hijacks the endocytic pathway to construct viral replication complexes on endosomes linked to the Golgi complex (GC). Then, analyzing crucial proteins involved in the secretory pathway, we showed the essential requirement of Rab1b, the Rab1b downstream effector Golgi-specific BFA resistance factor 1 (GBF1), and its substrate, the small GTPase ADP-ribosylation factor 1 (ARF1), for IBDV replication. In the current work, we focused on elucidating the IBDV assembly sites. We show that viral assembly occurs within single-membrane compartments closely associated with endoplasmic reticulum (ER) membranes, though we failed to elucidate the exact nature of the virus-wrapping membranes. Additionally, we show that IBDV infection promotes the stress of the ER, characterized by an accumulation of the chaperone binding protein (BiP) and lipid droplets (LDs) in the host cells. Overall, our results represent further original data showing the interplay between IBDV and the secretory pathway, making a substantial contribution to the field of birnaviruses–host cell interactions. |
| format | Artículo |
| id | INTA15525 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA155252023-10-11T15:00:45Z Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation Frontini-López, Yesica R. Rivera, Lautaro Pocognoni, Cristian A. Roldán, Julieta Suyai Colombo, Maria Isabel Uhart, Marina Delgui, Laura Ruth Infectious Bursal Disease Virus Endoplasmic Reticulum Lipids Virus Bursitis Infecciosa Retículo Endoplasmático Lípidos Gumboro illness is caused by the highly contagious immunosuppressive infectious bursal disease virus (IBDV), which affects the poultry industry globally. We have previously shown that IBDV hijacks the endocytic pathway to construct viral replication complexes on endosomes linked to the Golgi complex (GC). Then, analyzing crucial proteins involved in the secretory pathway, we showed the essential requirement of Rab1b, the Rab1b downstream effector Golgi-specific BFA resistance factor 1 (GBF1), and its substrate, the small GTPase ADP-ribosylation factor 1 (ARF1), for IBDV replication. In the current work, we focused on elucidating the IBDV assembly sites. We show that viral assembly occurs within single-membrane compartments closely associated with endoplasmic reticulum (ER) membranes, though we failed to elucidate the exact nature of the virus-wrapping membranes. Additionally, we show that IBDV infection promotes the stress of the ER, characterized by an accumulation of the chaperone binding protein (BiP) and lipid droplets (LDs) in the host cells. Overall, our results represent further original data showing the interplay between IBDV and the secretory pathway, making a substantial contribution to the field of birnaviruses–host cell interactions. Instituto de Virología Fil: Frontini-López, Yesica R. Universidad Nacional de Cuyo. Instituto de Histología y Embriología de Mendoza (IHEM); Argentina Fil: Frontini-López, Yesica R. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivera, Lautaro. Universidad Nacional de Cuyo. Instituto de Histología y Embriología de Mendoza (IHEM); Argentina Fil: Rivera, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pocognoni, Cristian A. Universidad Nacional de Cuyo. Instituto de Histología y Embriología de Mendoza (IHEM); Argentina Fil: Pocognoni, Cristian A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pocognoni, Cristian A. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Roldán, Julieta Suyai. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Roldán, Julieta Suyai. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colombo, Maria Isabel. Universidad Nacional de Cuyo. Instituto de Histología y Embriología de Mendoza (IHEM); Argentina Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colombo, Maria Isabel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Uhart, Marina. Universidad Nacional de Cuyo. Instituto de Histología y Embriología de Mendoza (IHEM); Argentina Fil: Uhart, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delgui, Laura Ruth. Universidad Nacional de Cuyo. Instituto de Histología y Embriología de Mendoza (IHEM); Argentina Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delgui, Laura Ruth. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina 2023-10-11T14:57:07Z 2023-10-11T14:57:07Z 2023-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15525 https://www.mdpi.com/1999-4915/15/6/1295 1999-4915 https://doi.org/10.3390/v15061295 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 15 (6) : 1295 (Junio 2023) |
| spellingShingle | Infectious Bursal Disease Virus Endoplasmic Reticulum Lipids Virus Bursitis Infecciosa Retículo Endoplasmático Lípidos Frontini-López, Yesica R. Rivera, Lautaro Pocognoni, Cristian A. Roldán, Julieta Suyai Colombo, Maria Isabel Uhart, Marina Delgui, Laura Ruth Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| title | Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| title_full | Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| title_fullStr | Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| title_full_unstemmed | Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| title_short | Infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| title_sort | infectious bursal disease virus assembly causes endoplasmic reticulum stress and lipid droplet accumulation |
| topic | Infectious Bursal Disease Virus Endoplasmic Reticulum Lipids Virus Bursitis Infecciosa Retículo Endoplasmático Lípidos |
| url | http://hdl.handle.net/20.500.12123/15525 https://www.mdpi.com/1999-4915/15/6/1295 https://doi.org/10.3390/v15061295 |
| work_keys_str_mv | AT frontinilopezyesicar infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation AT riveralautaro infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation AT pocognonicristiana infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation AT roldanjulietasuyai infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation AT colombomariaisabel infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation AT uhartmarina infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation AT delguilauraruth infectiousbursaldiseasevirusassemblycausesendoplasmicreticulumstressandlipiddropletaccumulation |