On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
Odonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15488 https://www.mdpi.com/2075-4450/13/12/1159 https://doi.org/10.3390/insects13121159 |
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| author | Mola, Liliana M. Vrbová, Iva Tosto, Daniela Sandra Zrzavá, Magda Marec, František |
| author_browse | Marec, František Mola, Liliana M. Tosto, Daniela Sandra Vrbová, Iva Zrzavá, Magda |
| author_facet | Mola, Liliana M. Vrbová, Iva Tosto, Daniela Sandra Zrzavá, Magda Marec, František |
| author_sort | Mola, Liliana M. |
| collection | INTA Digital |
| description | Odonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed the distribution of the nucleolar organizer region (NOR) using FISH with rDNA probes in Rhionaeschna bonariensis (n = 12 + neo-XY), R. planaltica (n = 7 + neo-XY), and Aeshna cyanea (n = 13 + X0). In R. bonariensis and A. cyanea, the NOR is located on a large pair of autosomes, which have a secondary constriction in the latter species. In R. planaltica, the NOR is located on the ancestral part of the neo-X chromosome. Meiotic analysis and FISH results in R. planaltica led to the conclusion that the neo-XY system arose by insertion of the ancestral X chromosome into an autosome. Genomic in situ hybridization, performed for the first time in Odonata, highlighted the entire neo-Y chromosome in meiosis of R. bonariensis, suggesting that it consists mainly of repetitive DNA. This feature and the terminal chiasma localization suggest an ancient origin of the neo-XY system. Our study provides new information on the origin and evolution of neo-sex chromosomes in Odonata, including new types of chromosomal rearrangements, NOR transposition, and heterochromatin accumulation. |
| format | Artículo |
| id | INTA15488 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA154882023-10-10T10:15:49Z On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) Mola, Liliana M. Vrbová, Iva Tosto, Daniela Sandra Zrzavá, Magda Marec, František Anisoptera (Insecta) Ribosomal DNA Meiosis ADN Ribosomal Rhionaeschna bonariensis Aeshnidae Evolución de los cromosomas neosexuales Neo-sex Chromosome Evolution Odonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed the distribution of the nucleolar organizer region (NOR) using FISH with rDNA probes in Rhionaeschna bonariensis (n = 12 + neo-XY), R. planaltica (n = 7 + neo-XY), and Aeshna cyanea (n = 13 + X0). In R. bonariensis and A. cyanea, the NOR is located on a large pair of autosomes, which have a secondary constriction in the latter species. In R. planaltica, the NOR is located on the ancestral part of the neo-X chromosome. Meiotic analysis and FISH results in R. planaltica led to the conclusion that the neo-XY system arose by insertion of the ancestral X chromosome into an autosome. Genomic in situ hybridization, performed for the first time in Odonata, highlighted the entire neo-Y chromosome in meiosis of R. bonariensis, suggesting that it consists mainly of repetitive DNA. This feature and the terminal chiasma localization suggest an ancient origin of the neo-XY system. Our study provides new information on the origin and evolution of neo-sex chromosomes in Odonata, including new types of chromosomal rearrangements, NOR transposition, and heterochromatin accumulation. Instituto de Biotecnología Fil: Mola, Liliana M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Laboratorio de Citogenética y Evolución; Argentina Fil: Mola, Liliana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vrbová, Iva. Biology Centre CAS. Institute of Entomology; República Checa Fil: Vrbová, Iva. Biology Centre CAS. Institute of Plant Molecular Biology; República Checa Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zrzavá, Magda. Biology Centre CAS. Institute of Entomology; República Checa Fil: Zrzavá, Magda. University of South Bohemia. Faculty of Science. Department of Molecular Biology and Genetics; República Checa Fil: Marec, Frantisek. Biology Centre CAS. Institute of Entomology; República Checa 2023-10-10T10:10:59Z 2023-10-10T10:10:59Z 2022-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15488 https://www.mdpi.com/2075-4450/13/12/1159 2075-4450 https://doi.org/10.3390/insects13121159 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Insects 13 (12) : 1159 (Diciembre 2022) |
| spellingShingle | Anisoptera (Insecta) Ribosomal DNA Meiosis ADN Ribosomal Rhionaeschna bonariensis Aeshnidae Evolución de los cromosomas neosexuales Neo-sex Chromosome Evolution Mola, Liliana M. Vrbová, Iva Tosto, Daniela Sandra Zrzavá, Magda Marec, František On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) |
| title | On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) |
| title_full | On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) |
| title_fullStr | On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) |
| title_full_unstemmed | On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) |
| title_short | On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata) |
| title_sort | on the origin of neo sex chromosomes in the neotropical dragonflies rhionaeschna bonariensis and r planaltica aeshnidae odonata |
| topic | Anisoptera (Insecta) Ribosomal DNA Meiosis ADN Ribosomal Rhionaeschna bonariensis Aeshnidae Evolución de los cromosomas neosexuales Neo-sex Chromosome Evolution |
| url | http://hdl.handle.net/20.500.12123/15488 https://www.mdpi.com/2075-4450/13/12/1159 https://doi.org/10.3390/insects13121159 |
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