The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis
Dry leaves and twigs of yerba mate are widely infusion-consumed in southern Southamerica. Endemic and adapted to the Atlantic Forest, its extensive full-sun monoculture links to diverse biotic (pest, pathogens) and abiotic stresses (solar radiation, drought), impacting its productivity, ecology and...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/15430 https://www.scielo.br/j/rod/a/8bqJkPHtMZv9bShDP7C9Z7g/?lang=en https://doi.org/10.1590/2175-7860202374020 |
| _version_ | 1855037316096065536 |
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| author | Aguilera, Patricia Mabel Debat, Humberto Julio Castrillo, María Lorena Bich, Gustavo Angel Grabiele, Mauro |
| author_browse | Aguilera, Patricia Mabel Bich, Gustavo Angel Castrillo, María Lorena Debat, Humberto Julio Grabiele, Mauro |
| author_facet | Aguilera, Patricia Mabel Debat, Humberto Julio Castrillo, María Lorena Bich, Gustavo Angel Grabiele, Mauro |
| author_sort | Aguilera, Patricia Mabel |
| collection | INTA Digital |
| description | Dry leaves and twigs of yerba mate are widely infusion-consumed in southern Southamerica. Endemic and adapted to the Atlantic Forest, its extensive full-sun monoculture links to diverse biotic (pest, pathogens) and abiotic stresses (solar radiation, drought), impacting its productivity, ecology and socioeconomic niche. We focused in comprehensively characterize the DNAJ gene family in yerba mate to predict its possible roles on development and diverse stress responses to further assist crop manage. Our results suggest that yerba mate DNAJ proteins account 140 diverse members of six structural types displaying potential variable roles in protein homeostasis control. We were able to classify them into 51 distinct orthology groups, in agreement to Arabidopsis, and performed translational genomics of function, localization, expression and stress responsiveness data. Genome mapping and expression analysis indicated that yerba mate DNAJ genes differ in expression, nucleotide composition, length and exon-intron structure. Intronless or few introns genes -linked to rapid stress response- accounted 85 DNAJs. Promoters of DNAJ genes harbored a 73.2% of cis-acting regulatory elements involved in response to diverse stresses, hormones and light, simultaneously. We hypothesize that yerba mate DNAJs assist to plant survival during multiple stresses linked to current dominant agroecosystem but promote its growth under shade. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15430 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| publisherStr | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| record_format | dspace |
| spelling | INTA154302023-10-05T11:07:16Z The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis Aguilera, Patricia Mabel Debat, Humberto Julio Castrillo, María Lorena Bich, Gustavo Angel Grabiele, Mauro Genomics Ilex Paraguariensis Mate Genómica Chaperones Crop Tree Stress Genes Translational Genomics Yerba Mate Chaperonas Arbol Cultivado Genes de Estrés Genómica Traslativa Dry leaves and twigs of yerba mate are widely infusion-consumed in southern Southamerica. Endemic and adapted to the Atlantic Forest, its extensive full-sun monoculture links to diverse biotic (pest, pathogens) and abiotic stresses (solar radiation, drought), impacting its productivity, ecology and socioeconomic niche. We focused in comprehensively characterize the DNAJ gene family in yerba mate to predict its possible roles on development and diverse stress responses to further assist crop manage. Our results suggest that yerba mate DNAJ proteins account 140 diverse members of six structural types displaying potential variable roles in protein homeostasis control. We were able to classify them into 51 distinct orthology groups, in agreement to Arabidopsis, and performed translational genomics of function, localization, expression and stress responsiveness data. Genome mapping and expression analysis indicated that yerba mate DNAJ genes differ in expression, nucleotide composition, length and exon-intron structure. Intronless or few introns genes -linked to rapid stress response- accounted 85 DNAJs. Promoters of DNAJ genes harbored a 73.2% of cis-acting regulatory elements involved in response to diverse stresses, hormones and light, simultaneously. We hypothesize that yerba mate DNAJs assist to plant survival during multiple stresses linked to current dominant agroecosystem but promote its growth under shade. Las hojas y ramitas secas de yerba mate son ampliamente consumidas como infusión en el sur de Sudamérica. Endémica y adaptada a la Mata Atlántica, el monocultivo extensivo de esta planta a pleno sol se vincula a diversos estreses bióticos (pestes, patógenos) y abióticos (radiación solar, sequía) que impactan en su productividad, ecología y nicho socioeconómico. El objetivo de este trabajo fue caracterizar exhaustivamente la familia de genes DNAJ en yerba mate a fin de predecir sus posibles roles en el desarrollo y en las respuestas a diversos estreses para así contribuir al manejo del cultivo. Nuestros resultados sugieren que las proteínas DNAJ de yerba mate contabilizan 140 miembros diversos de seis tipos estructurales, con diferentes roles potenciales en el control de la homeostasis proteica. Asimismo, fueron clasificadas en 51 grupos ortólogos distintos, de acuerdo con Arabidopsis, y se realizó la genómica traslativa de datos de función, localización, expresión y respuesta a estrés. El mapeo genómico y los análisis de expresión indicaron que los genes DNAJ de yerba mate difieren en expresión, composición nucleotídica, longitud y estructura exón-intrón. Se encontró que 85 genes DNAJ no presentan o poseen pocos intrones -ligados a una rápida respuesta a estrés-. Los promotores de genes DNAJ albergan un 73,2 % de elementos reguladores en cis involucrados en respuesta a diversos estreses, hormonas y luz, simultáneamente. Así, proponemos que las DNAJs de yerba mate asisten a la planta en su supervivencia durante múltiples estreses ligados al actual agroecosistema dominante, mientras que bajo sombra promueven su crecimiento. Instituto de Patología Vegetal Fil: Aguilera, Patricia Mabel. Universidad Nacional de Misiones. Instituto de Biología Subtropical.(UNaM-CONICET) Instituto de Biotecnología Misiones; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Sala de Biología Computacional; Argentina Fil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Sala de Biología Computacional; Argentina Fil: Grabiele, Mauro. Universidad Nacional de Misiones. Instituto de Biología Subtropical (UNaM-CONICET). Instituto de Biotecnología Misiones; Argentina 2023-10-05T11:01:00Z 2023-10-05T11:01:00Z 2023-05-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15430 https://www.scielo.br/j/rod/a/8bqJkPHtMZv9bShDP7C9Z7g/?lang=en 0370-6583 2175-7860 https://doi.org/10.1590/2175-7860202374020 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto de Pesquisas Jardim Botânico do Rio de Janeiro Rodriguésia 74 : e00492022 (2023) |
| spellingShingle | Genomics Ilex Paraguariensis Mate Genómica Chaperones Crop Tree Stress Genes Translational Genomics Yerba Mate Chaperonas Arbol Cultivado Genes de Estrés Genómica Traslativa Aguilera, Patricia Mabel Debat, Humberto Julio Castrillo, María Lorena Bich, Gustavo Angel Grabiele, Mauro The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis |
| title | The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis |
| title_full | The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis |
| title_fullStr | The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis |
| title_full_unstemmed | The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis |
| title_short | The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis |
| title_sort | dnaj gene family in yerba mate ilex paraguariensis genome wide identification structural characterization orthology based classification and expression analysis |
| topic | Genomics Ilex Paraguariensis Mate Genómica Chaperones Crop Tree Stress Genes Translational Genomics Yerba Mate Chaperonas Arbol Cultivado Genes de Estrés Genómica Traslativa |
| url | http://hdl.handle.net/20.500.12123/15430 https://www.scielo.br/j/rod/a/8bqJkPHtMZv9bShDP7C9Z7g/?lang=en https://doi.org/10.1590/2175-7860202374020 |
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