Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications,...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15366 https://link.springer.com/article/10.1007/s11262-009-0345-2 https://doi.org/10.1007/s11262-009-0345-2 |
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| author | Distefano, Ana Julia Maldonado, Sara Beatriz Hopp, Horacio Esteban Del Vas, Mariana |
| author_browse | Del Vas, Mariana Distefano, Ana Julia Hopp, Horacio Esteban Maldonado, Sara Beatriz |
| author_facet | Distefano, Ana Julia Maldonado, Sara Beatriz Hopp, Horacio Esteban Del Vas, Mariana |
| author_sort | Distefano, Ana Julia |
| collection | INTA Digital |
| description | Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15366 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA153662023-09-28T15:28:13Z Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein Distefano, Ana Julia Maldonado, Sara Beatriz Hopp, Horacio Esteban Del Vas, Mariana Fijivirus Maíz Proteínas Estructurales Maize Structural Proteins Mal de Río Cuarto Virus Capsid Virus del Mal de Río Cuarto Cápside Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. Instituto de Biotecnología Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Distefano, Ana Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2023-09-28T15:19:34Z 2023-09-28T15:19:34Z 2009-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15366 https://link.springer.com/article/10.1007/s11262-009-0345-2 1572-994X https://doi.org/10.1007/s11262-009-0345-2 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Virus Genes 38 (3) : 455-460 (Junio 2009) |
| spellingShingle | Fijivirus Maíz Proteínas Estructurales Maize Structural Proteins Mal de Río Cuarto Virus Capsid Virus del Mal de Río Cuarto Cápside Distefano, Ana Julia Maldonado, Sara Beatriz Hopp, Horacio Esteban Del Vas, Mariana Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title | Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_full | Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_fullStr | Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_full_unstemmed | Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_short | Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_sort | mal de rio cuarto virus mrcv genomic segment s3 codes for the major core capsid protein |
| topic | Fijivirus Maíz Proteínas Estructurales Maize Structural Proteins Mal de Río Cuarto Virus Capsid Virus del Mal de Río Cuarto Cápside |
| url | http://hdl.handle.net/20.500.12123/15366 https://link.springer.com/article/10.1007/s11262-009-0345-2 https://doi.org/10.1007/s11262-009-0345-2 |
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