Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts
Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed v...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15341 https://www.sciencedirect.com/science/article/pii/S0168170210001942 https://doi.org/10.1016/j.virusres.2010.06.010 |
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| author | Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Veronica Distefano, Ana Julia Llauger, Gabriela Taboga, Oscar Alberto Hopp, Horacio Esteban Del Vas, Mariana |
| author_browse | Del Vas, Mariana Distefano, Ana Julia Hopp, Horacio Esteban Llauger, Gabriela Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Veronica Taboga, Oscar Alberto |
| author_facet | Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Veronica Distefano, Ana Julia Llauger, Gabriela Taboga, Oscar Alberto Hopp, Horacio Esteban Del Vas, Mariana |
| author_sort | Maroniche, Guillermo Andrés |
| collection | INTA Digital |
| description | Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15341 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA153412023-09-27T14:23:00Z Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Veronica Distefano, Ana Julia Llauger, Gabriela Taboga, Oscar Alberto Hopp, Horacio Esteban Del Vas, Mariana Fijivirus Reovirus ssRNA viruses ATPase ATPasa Mal de Río Cuarto Virus Viroplasm Virus del mal de Río Cuarto Viroplasma Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins. Instituto de Biotecnología Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mongelli, Vanesa Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Distefano, Ana Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Llauger, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2023-09-27T14:18:32Z 2023-09-27T14:18:32Z 2010-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15341 https://www.sciencedirect.com/science/article/pii/S0168170210001942 1872-7492 https://doi.org/10.1016/j.virusres.2010.06.010 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Virus Research 152 (1-2) : 96-103 (Septiembre 2010) |
| spellingShingle | Fijivirus Reovirus ssRNA viruses ATPase ATPasa Mal de Río Cuarto Virus Viroplasm Virus del mal de Río Cuarto Viroplasma Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Veronica Distefano, Ana Julia Llauger, Gabriela Taboga, Oscar Alberto Hopp, Horacio Esteban Del Vas, Mariana Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
| title | Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
| title_full | Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
| title_fullStr | Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
| title_full_unstemmed | Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
| title_short | Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
| title_sort | functional and biochemical properties of mal de rio cuarto virus fijivirus reoviridae p9 1 viroplasm protein show further similarities to animal reovirus counterparts |
| topic | Fijivirus Reovirus ssRNA viruses ATPase ATPasa Mal de Río Cuarto Virus Viroplasm Virus del mal de Río Cuarto Viroplasma |
| url | http://hdl.handle.net/20.500.12123/15341 https://www.sciencedirect.com/science/article/pii/S0168170210001942 https://doi.org/10.1016/j.virusres.2010.06.010 |
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