Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
Anaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon rem...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/15302 https://www.mdpi.com/2076-0817/12/8/1010 https://doi.org/10.3390/pathogens12081010 |
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| author | De La Fourniere, Sofia Guillemi, Eliana Carolina Paoletta, Martina Pérez, Agustina Ericlee Obregón, Dasiel Cabezas-Cruz, Alejandro Sarmiento, Nestor Fabian Farber, Marisa Diana |
| author_browse | Cabezas-Cruz, Alejandro De La Fourniere, Sofia Farber, Marisa Diana Guillemi, Eliana Carolina Obregón, Dasiel Paoletta, Martina Pérez, Agustina Ericlee Sarmiento, Nestor Fabian |
| author_facet | De La Fourniere, Sofia Guillemi, Eliana Carolina Paoletta, Martina Pérez, Agustina Ericlee Obregón, Dasiel Cabezas-Cruz, Alejandro Sarmiento, Nestor Fabian Farber, Marisa Diana |
| author_sort | De La Fourniere, Sofia |
| collection | INTA Digital |
| description | Anaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of R. microplus larvae vertically infected with A. marginale to transmit the bacterium to a naïve bovine. A subgroup of engorged female ticks collected from an A. marginale-positive animal was dissected and the presence of the pathogen in its tissues was confirmed. A second subgroup of ticks was placed under controlled conditions for oviposition. After confirming the presence of A. marginale in the hatched larvae, an experimental infestation assay was conducted. Larvae were placed on an A. marginale-free splenectomized calf. The bacterium was detected in the experimentally infested bovine 22 days post-infestation. We analyzed the A. marginale diversity throughout the transmission cycle using the molecular marker MSP1a. Different genotypes were detected in the mammalian and arthropod hosts showing a reduction of strain diversity along the transmission process. Our results demonstrate the vertical transmission of A. marginale from R. microplus females to its larvae, their vector competence to transmit the pathogen, and a bottleneck in A. marginale strain diversity. |
| format | Artículo |
| id | INTA15302 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA153022023-09-25T14:50:27Z Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity De La Fourniere, Sofia Guillemi, Eliana Carolina Paoletta, Martina Pérez, Agustina Ericlee Obregón, Dasiel Cabezas-Cruz, Alejandro Sarmiento, Nestor Fabian Farber, Marisa Diana Anaplasma marginale Rhipicephalus Vectors Vectores Anaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of R. microplus larvae vertically infected with A. marginale to transmit the bacterium to a naïve bovine. A subgroup of engorged female ticks collected from an A. marginale-positive animal was dissected and the presence of the pathogen in its tissues was confirmed. A second subgroup of ticks was placed under controlled conditions for oviposition. After confirming the presence of A. marginale in the hatched larvae, an experimental infestation assay was conducted. Larvae were placed on an A. marginale-free splenectomized calf. The bacterium was detected in the experimentally infested bovine 22 days post-infestation. We analyzed the A. marginale diversity throughout the transmission cycle using the molecular marker MSP1a. Different genotypes were detected in the mammalian and arthropod hosts showing a reduction of strain diversity along the transmission process. Our results demonstrate the vertical transmission of A. marginale from R. microplus females to its larvae, their vector competence to transmit the pathogen, and a bottleneck in A. marginale strain diversity. Instituto de Biotecnología Fil: De La Fourniere, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: De La Fourniere, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Paoletta, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; Canadá Fil: Cabezas-Cruz, Alejandro. Agence Nationale de Sécurité Sanitaire de l’Alimentation (ANSES). Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). Ecole Nationale Vétérinarie d’Alfort. Laboratoire de Santé Animale; Francia Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-09-25T14:42:05Z 2023-09-25T14:42:05Z 2023-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15302 https://www.mdpi.com/2076-0817/12/8/1010 2076-0817 https://doi.org/10.3390/pathogens12081010 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 12 (8) : 1010 (Agosto 2023) |
| spellingShingle | Anaplasma marginale Rhipicephalus Vectors Vectores De La Fourniere, Sofia Guillemi, Eliana Carolina Paoletta, Martina Pérez, Agustina Ericlee Obregón, Dasiel Cabezas-Cruz, Alejandro Sarmiento, Nestor Fabian Farber, Marisa Diana Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity |
| title | Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity |
| title_full | Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity |
| title_fullStr | Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity |
| title_full_unstemmed | Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity |
| title_short | Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity |
| title_sort | transovarial transmission of anaplasma marginale in rhipicephalus boophilus microplus ticks results in a bottleneck for strain diversity |
| topic | Anaplasma marginale Rhipicephalus Vectors Vectores |
| url | http://hdl.handle.net/20.500.12123/15302 https://www.mdpi.com/2076-0817/12/8/1010 https://doi.org/10.3390/pathogens12081010 |
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