Phenotypic effects of different doses of physical and chemical mutagens in cotton plants
Induced mutation techniques have been effectively used to obtain new variability in crop plants. Mutations are the primary source of genetic variability and therefore, some control over their frequency can be considered a valuable tool for plant breeding. Mutagens can be grouped into two broad categ...
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| Format: | Artículo |
| Language: | Inglés |
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Ediciones INTA
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15298 https://doi.org/10.58149/kbvg-ne23 |
| _version_ | 1855485552098279424 |
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| author | Winkler, Horacio Martín Cereijo, Antonela Estefania Scarpin, Gonzalo Joel Dileo, Pablo Nahuel Muchut, Robertino Jose Roeschlin, Roxana Andrea Lorenzini, Fernando Paytas, Marcelo Javier Landau, Alejandra Mabel |
| author_browse | Cereijo, Antonela Estefania Dileo, Pablo Nahuel Landau, Alejandra Mabel Lorenzini, Fernando Muchut, Robertino Jose Paytas, Marcelo Javier Roeschlin, Roxana Andrea Scarpin, Gonzalo Joel Winkler, Horacio Martín |
| author_facet | Winkler, Horacio Martín Cereijo, Antonela Estefania Scarpin, Gonzalo Joel Dileo, Pablo Nahuel Muchut, Robertino Jose Roeschlin, Roxana Andrea Lorenzini, Fernando Paytas, Marcelo Javier Landau, Alejandra Mabel |
| author_sort | Winkler, Horacio Martín |
| collection | INTA Digital |
| description | Induced mutation techniques have been effectively used to obtain new variability in crop plants. Mutations are the primary source of genetic variability and therefore, some control over their frequency can be considered a valuable tool for plant breeding. Mutagens can be grouped into two broad categories according to their nature: physical and chemical. Doses must be optimized to increase the chances of generating mutants and achieve a population of mutagenized plants that allow a successful selection of mutants of interest. The aim of this study was to test the sensitivity of different mutagenic agents on Guazuncho 3 variety cotton seeds. Four doses of each treatment were evaluated and different phenotypic traits were recorded. The greatest effects on germination percentage, germination velocity index, plant survival and somatic effects were observed in treatments with ethyl methanesulfonate (EMS) as the doses increased. Lower but significant effects were recorded in sodium azide (SA) treatments and no significant differences were observed for X-rays and combined treatments (SA+X-rays). The effects on growth rate were significant in chemical treatments with R2 = 0.84 for SA and R2 = 0.97 for EMS. In addition, it was observed that the EMS and X-rays treatments caused a greater number of off-type plants and chlorotic irregular-shaped spots. The results of this preliminary study determined the mutagens doses to be used on Guazuncho 3 cotton seeds, to generate an M1 population for developing a mutation breeding program. |
| format | Artículo |
| id | INTA15298 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Ediciones INTA |
| publisherStr | Ediciones INTA |
| record_format | dspace |
| spelling | INTA152982023-09-22T16:11:41Z Phenotypic effects of different doses of physical and chemical mutagens in cotton plants Winkler, Horacio Martín Cereijo, Antonela Estefania Scarpin, Gonzalo Joel Dileo, Pablo Nahuel Muchut, Robertino Jose Roeschlin, Roxana Andrea Lorenzini, Fernando Paytas, Marcelo Javier Landau, Alejandra Mabel Algodón Gossypium hirsutum Fenotipos Mutación Inducida Mutágenos Cotton Phenotypes Induced Mutation Mutagens Induced mutation techniques have been effectively used to obtain new variability in crop plants. Mutations are the primary source of genetic variability and therefore, some control over their frequency can be considered a valuable tool for plant breeding. Mutagens can be grouped into two broad categories according to their nature: physical and chemical. Doses must be optimized to increase the chances of generating mutants and achieve a population of mutagenized plants that allow a successful selection of mutants of interest. The aim of this study was to test the sensitivity of different mutagenic agents on Guazuncho 3 variety cotton seeds. Four doses of each treatment were evaluated and different phenotypic traits were recorded. The greatest effects on germination percentage, germination velocity index, plant survival and somatic effects were observed in treatments with ethyl methanesulfonate (EMS) as the doses increased. Lower but significant effects were recorded in sodium azide (SA) treatments and no significant differences were observed for X-rays and combined treatments (SA+X-rays). The effects on growth rate were significant in chemical treatments with R2 = 0.84 for SA and R2 = 0.97 for EMS. In addition, it was observed that the EMS and X-rays treatments caused a greater number of off-type plants and chlorotic irregular-shaped spots. The results of this preliminary study determined the mutagens doses to be used on Guazuncho 3 cotton seeds, to generate an M1 population for developing a mutation breeding program. Las técnicas de mutaciones inducidas se han utilizado eficazmente para obtener nueva variabilidad en las plantas de cultivo. Las mutaciones son la principal fuente de variabilidad genética y, por lo tanto, cierto control sobre su frecuencia puede considerarse una herramienta valiosa para el fitomejoramiento. Los mutágenos pueden agruparse en dos grandes categorías según su naturaleza: físicos y químicos. Las dosis deben optimizarse para aumentar las posibilidades de generar mutantes y lograr una población de plantas mutagenizadas que permita una selección exitosa de mutantes de interés. El objetivo de este estudio fue evaluar la sensibilidad de diferentes agentes mutagénicos en semillas de algodón de la variedad Guazuncho 3. Se evaluaron cuatro dosis de cada tratamiento y se registraron diferentes caracteres fenotípicos. Los mayores efectos sobre el porcentaje de germinación, el índice de velocidad de germinación, la supervivencia de las plantas y los efectos somáticos se observaron en los tratamientos con metanosulfonato de etilo (EMS) a medida que aumentaban las dosis. Se registraron efectos menores pero significativos en los tratamientos con azida sódica (SA) y no se observaron diferencias significativas en los tratamientos con rayos X y combinados (SA + rayos X). Los efectos sobre la tasa de crecimiento fueron significativos en los tratamientos químicos con R2 = 0,84 para SA y R2 = 0,97 para EMS. Además, se observó que los tratamientos con EMS y rayos X provocaron un mayor número de plantas fuera de tipo y manchas cloróticas de forma irregular. Los resultados de este estudio preliminar determinaron las dosis de mutágenos a utilizar en semillas de algodón Guazuncho 3, con el fin de generar una población M1 para desarrollar un programa de mejoramiento de mutaciones. EEA Reconquista Fil: Winkler, Horacio Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Winkler, Horacio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cereijo, Antonela Estefanía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Cereijo, Antonela Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scarpin, Gonzalo Joel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Dileo, Pablo Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Muchut, Robertino José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Muchut, Robertino José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Roeschlin, Roxana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Roeschlin, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lorenzini, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Lorenzini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paytas, Marcelo Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2023-09-22T16:08:22Z 2023-09-22T16:08:22Z 2023-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15298 0325-8718 1669-2314 https://doi.org/10.58149/kbvg-ne23 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001, Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Ediciones INTA RIA 49 (2) : 71-84. (septiembre 2023) |
| spellingShingle | Algodón Gossypium hirsutum Fenotipos Mutación Inducida Mutágenos Cotton Phenotypes Induced Mutation Mutagens Winkler, Horacio Martín Cereijo, Antonela Estefania Scarpin, Gonzalo Joel Dileo, Pablo Nahuel Muchut, Robertino Jose Roeschlin, Roxana Andrea Lorenzini, Fernando Paytas, Marcelo Javier Landau, Alejandra Mabel Phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| title | Phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| title_full | Phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| title_fullStr | Phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| title_full_unstemmed | Phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| title_short | Phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| title_sort | phenotypic effects of different doses of physical and chemical mutagens in cotton plants |
| topic | Algodón Gossypium hirsutum Fenotipos Mutación Inducida Mutágenos Cotton Phenotypes Induced Mutation Mutagens |
| url | http://hdl.handle.net/20.500.12123/15298 https://doi.org/10.58149/kbvg-ne23 |
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