Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina
A total of 268 Bacillus thuringiensis strains obtained from different sources of Argentina were analyzed to determine the diversity and distribution of the cry1, cry2, cry8, cry9 and vip3A genes encoding for lepidopteran-specific insecticidal proteins. Twin strains were excluded. Ten different profi...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1527 http://www.sciencedirect.com/science/article/pii/S0325754117300391?via%3Dihub https://doi.org/10.1016/j.ram.2017.02.003 |
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| author | Sauka, Diego Hernan Benintende, Graciela Beatriz |
| author_browse | Benintende, Graciela Beatriz Sauka, Diego Hernan |
| author_facet | Sauka, Diego Hernan Benintende, Graciela Beatriz |
| author_sort | Sauka, Diego Hernan |
| collection | INTA Digital |
| description | A total of 268 Bacillus thuringiensis strains obtained from different sources of Argentina were analyzed to determine the diversity and distribution of the cry1, cry2, cry8, cry9 and vip3A genes encoding for lepidopteran-specific insecticidal proteins. Twin strains were excluded. Ten different profiles were detected among the 80 selected B. thuringiensis strains. Two of these profiles (cry1Aa, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa (35/80), and cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa (25/80)) pooled 75% of the strains. The existence of this low diversity is rare, since in most of the studied collections a great diversity of insecticidal toxin gene profiles has been described. In addition, the most frequently detected profile was also most frequently derived from soil (70%), stored product dust (59%) and spider webs (50%). In contrast, the cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa profiles were mainly detected in strains isolated from leaves (40%) and dead insect larvae (50%). Six of the identified insecticidal toxin gene profiles were discovered in strains isolated from stored product dust and leaves indicating higher diversity of profiles in these kinds of sources than in others. Some strains with high insecticidal activity against Epinotia aporema (Lepidoptera) larvae were identified, which is important to explore future microbial strategies for the control of this crop pest in the region |
| format | info:eu-repo/semantics/article |
| id | INTA1527 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA15272018-01-08T14:21:05Z Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina Sauka, Diego Hernan Benintende, Graciela Beatriz Bacillus Thuringiensis Lepidoptera Epinotia Aporema Genes Genética Genetics Argentina A total of 268 Bacillus thuringiensis strains obtained from different sources of Argentina were analyzed to determine the diversity and distribution of the cry1, cry2, cry8, cry9 and vip3A genes encoding for lepidopteran-specific insecticidal proteins. Twin strains were excluded. Ten different profiles were detected among the 80 selected B. thuringiensis strains. Two of these profiles (cry1Aa, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa (35/80), and cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa (25/80)) pooled 75% of the strains. The existence of this low diversity is rare, since in most of the studied collections a great diversity of insecticidal toxin gene profiles has been described. In addition, the most frequently detected profile was also most frequently derived from soil (70%), stored product dust (59%) and spider webs (50%). In contrast, the cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa profiles were mainly detected in strains isolated from leaves (40%) and dead insect larvae (50%). Six of the identified insecticidal toxin gene profiles were discovered in strains isolated from stored product dust and leaves indicating higher diversity of profiles in these kinds of sources than in others. Some strains with high insecticidal activity against Epinotia aporema (Lepidoptera) larvae were identified, which is important to explore future microbial strategies for the control of this crop pest in the region Se analizaron 268 cepas de Bacillus thuringiensis obtenidas de diferentes fuentes de Argentina con el objeto de determinar la diversidad y distribución de genes cry1, cry2, cry8, cry9 y vip3A, que codifican proteínas insecticidas lepidóptero-específicas. Se excluyeron las cepas gemelas. Se detectaron solo diez perfiles diferentes entre los 80 B. thuringiensis seleccionados. Dos de estos perfiles, el cry1Aa, cry1Ac, cry1Ia, cry2Aa, cry2Ab y vip3Aa (35/80) y el cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab y vip3Aa (25/80), comprendieron el 75% de las cepas seleccionadas. La existencia de esta baja diversidad es una rareza, ya que en la mayor parte de las colecciones estudiadas se ha descrito una gran diversidad de perfiles de genes de toxinas insecticidas. El perfil detectado con mayor frecuencia se obtuvo principalmente de cepas procedentes de suelo (el 70% de los de esa fuente lo tenían), también fue mayoritario entre los procedentes de polvo de producto almacenado (59%) y en los que procedían de telas de araña (50%). En cambio, el perfil cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Aa, cry2Ab y vip3Aa se detectó principalmente en las cepas aisladas de hojas (40%) y de larvas de insectos muertos (50%). Seis de los perfiles identificados fueron encontrados en cepas aisladas de polvo de producto almacenado y de hojas, lo que indica una mayor diversidad de perfiles en estas fuentes que en otras. Se identificaron algunas cepas con alta actividad insecticida contra larvas de Epinotia aporema (Lepidoptera), hallazgo importante para explorar en el futuro estrategias microbianas para el control de esta plaga en la región. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2017-10-19T11:47:48Z 2017-10-19T11:47:48Z 2017-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1527 http://www.sciencedirect.com/science/article/pii/S0325754117300391?via%3Dihub 0325-7541 https://doi.org/10.1016/j.ram.2017.02.003 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina (nation) Revista argentina de microbiología 49 (3) : 273-281. (2017) |
| spellingShingle | Bacillus Thuringiensis Lepidoptera Epinotia Aporema Genes Genética Genetics Argentina Sauka, Diego Hernan Benintende, Graciela Beatriz Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina |
| title | Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina |
| title_full | Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina |
| title_fullStr | Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina |
| title_full_unstemmed | Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina |
| title_short | Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina |
| title_sort | diversity and distribution of lepidoptera specific toxin genes in bacillus thuringiensis strains from argentina |
| topic | Bacillus Thuringiensis Lepidoptera Epinotia Aporema Genes Genética Genetics Argentina |
| url | http://hdl.handle.net/20.500.12123/1527 http://www.sciencedirect.com/science/article/pii/S0325754117300391?via%3Dihub https://doi.org/10.1016/j.ram.2017.02.003 |
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