Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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National Academy of Sciences
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/15250 https://www.pnas.org/doi/10.1073/pnas.2300203120 https://doi.org/10.1073/pnas.2300203120 |
| _version_ | 1855485532472082432 |
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| author | Zhang, Junli LI, Chengxia Zhang, Wenjun Zhang, Xiaoqin Mo, Youngjun Tranquilli, Gabriela Vanzetti, Leonardo Sebastian Dubcovsky, Jorge |
| author_browse | Dubcovsky, Jorge LI, Chengxia Mo, Youngjun Tranquilli, Gabriela Vanzetti, Leonardo Sebastian Zhang, Junli Zhang, Wenjun Zhang, Xiaoqin |
| author_facet | Zhang, Junli LI, Chengxia Zhang, Wenjun Zhang, Xiaoqin Mo, Youngjun Tranquilli, Gabriela Vanzetti, Leonardo Sebastian Dubcovsky, Jorge |
| author_sort | Zhang, Junli |
| collection | INTA Digital |
| description | Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification,
validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a
plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat
protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties. |
| format | Artículo |
| id | INTA15250 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | INTA152502023-09-19T17:32:21Z Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) Zhang, Junli LI, Chengxia Zhang, Wenjun Zhang, Xiaoqin Mo, Youngjun Tranquilli, Gabriela Vanzetti, Leonardo Sebastian Dubcovsky, Jorge Plant Height Wheat Altura de la Planta Trigo DELLA PLATZ Variación Natural Natural Variation Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties. Fil: Zhang, Junli. University of California. Department of Plant Sciences; Estados Unidos Fil: Li, Chengxia. University of California. Department of Plant Sciences; Estados Unidos Fil: Zhang, Wenjun. University of California. Department of Plant Sciences; Estados Unidos Fil: Zhang, Xiaoqin. University of California. Department of Plant Sciences; Estados Unidos Fil: Mo, Youngjun. University of California. Department of Plant Sciences; Estados Unidos Fil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Vanzetti, Leonardo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos 2023-09-19T17:13:04Z 2023-09-19T17:13:04Z 2023-05-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15250 https://www.pnas.org/doi/10.1073/pnas.2300203120 0027-8424 https://doi.org/10.1073/pnas.2300203120 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf National Academy of Sciences PNAS 120 (19) : e2300203120 (May 2023) |
| spellingShingle | Plant Height Wheat Altura de la Planta Trigo DELLA PLATZ Variación Natural Natural Variation Zhang, Junli LI, Chengxia Zhang, Wenjun Zhang, Xiaoqin Mo, Youngjun Tranquilli, Gabriela Vanzetti, Leonardo Sebastian Dubcovsky, Jorge Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) |
| title | Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) |
| title_full | Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) |
| title_fullStr | Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) |
| title_full_unstemmed | Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) |
| title_short | Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) |
| title_sort | wheat plant height locus rht25 encodes a platz transcription factor that interacts with della rht1 |
| topic | Plant Height Wheat Altura de la Planta Trigo DELLA PLATZ Variación Natural Natural Variation |
| url | http://hdl.handle.net/20.500.12123/15250 https://www.pnas.org/doi/10.1073/pnas.2300203120 https://doi.org/10.1073/pnas.2300203120 |
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