Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
Our study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intesti...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15245 https://www.sciencedirect.com/science/article/abs/pii/S0147957123001133 https://doi.org/10.1016/j.cimid.2023.102055 |
| _version_ | 1855037279476645888 |
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| author | Soria, Mario Godano, Eduardo Ignacio Leiva, Leonardo Esteban Bueno, Dante Javier |
| author_browse | Bueno, Dante Javier Godano, Eduardo Ignacio Leiva, Leonardo Esteban Soria, Mario |
| author_facet | Soria, Mario Godano, Eduardo Ignacio Leiva, Leonardo Esteban Bueno, Dante Javier |
| author_sort | Soria, Mario |
| collection | INTA Digital |
| description | Our study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks. Furthermore, we looked for a biomarker of SG infection. Spleen, liver, ovarian follicle content, and bone marrow were found to be the best samples for SG isolation and the agreement between MacConkey-Salmonella Shigella agar was slight to excellent. The laying hens with SG isolation and rapid serum plate agglutination positive results showed a higher percentage of heterophils, heterophil/lymphocyte ratio and total white blood cells, and a lower percentage of lymphocytes than those with negative results. Furthermore, the positive fecal leukocyte samples had a higher percentage of heterophils, gamma-glutamyl transferase, total protein and globulin values than negative samples. Five biomarkers’ cut-offs are proposed to distinguish between laying hens positive and negative to SG isolation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15245 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA152452023-09-18T13:50:55Z Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens Soria, Mario Godano, Eduardo Ignacio Leiva, Leonardo Esteban Bueno, Dante Javier Salmonella Layer Chickens Animal Diseases Salmonella gallinarum Biochemical Analysis Serology Gallina Ponedora Enfermedades de los Animales Análisis Bioquímico Serología Our study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks. Furthermore, we looked for a biomarker of SG infection. Spleen, liver, ovarian follicle content, and bone marrow were found to be the best samples for SG isolation and the agreement between MacConkey-Salmonella Shigella agar was slight to excellent. The laying hens with SG isolation and rapid serum plate agglutination positive results showed a higher percentage of heterophils, heterophil/lymphocyte ratio and total white blood cells, and a lower percentage of lymphocytes than those with negative results. Furthermore, the positive fecal leukocyte samples had a higher percentage of heterophils, gamma-glutamyl transferase, total protein and globulin values than negative samples. Five biomarkers’ cut-offs are proposed to distinguish between laying hens positive and negative to SG isolation. EEA Concepción del Uruguay Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Soria, Mario Alberto. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; Argentina Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; Argentina 2023-09-18T13:45:34Z 2023-09-18T13:45:34Z 2023-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15245 https://www.sciencedirect.com/science/article/abs/pii/S0147957123001133 1878-1667 0147-9571 https://doi.org/10.1016/j.cimid.2023.102055 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 101 : 102055. (October 2023) |
| spellingShingle | Salmonella Layer Chickens Animal Diseases Salmonella gallinarum Biochemical Analysis Serology Gallina Ponedora Enfermedades de los Animales Análisis Bioquímico Serología Soria, Mario Godano, Eduardo Ignacio Leiva, Leonardo Esteban Bueno, Dante Javier Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| title | Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| title_full | Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| title_fullStr | Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| title_full_unstemmed | Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| title_short | Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| title_sort | relationship of salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens |
| topic | Salmonella Layer Chickens Animal Diseases Salmonella gallinarum Biochemical Analysis Serology Gallina Ponedora Enfermedades de los Animales Análisis Bioquímico Serología |
| url | http://hdl.handle.net/20.500.12123/15245 https://www.sciencedirect.com/science/article/abs/pii/S0147957123001133 https://doi.org/10.1016/j.cimid.2023.102055 |
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