Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs
Human rotavirus (HRV) is a leading cause of viral gastroenteritis in children across the globe. The virus has long been established as a pathogen of the gastrointestinal tract, targeting small intestine epithelial cells and leading to diarrhea, nausea, and vomiting. Recently, this classical infectio...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/15204 https://www.mdpi.com/1999-4915/15/9/1864 https://doi.org/10.3390/v15091864 |
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| author | Nyblade, Charlotte Zhou, Peng Frazier, Maggie Frazier, Annie Hensley, Casey Fantasia-Davis, Ariana Shahrudin, Shabihah Hoffer, Miranda Agbemabiese, Chantal Ama LaRue, Lauren Barro, Mario Patton, John T. Parreño, Gladys Viviana Yuan, Lijuan |
| author_browse | Agbemabiese, Chantal Ama Barro, Mario Fantasia-Davis, Ariana Frazier, Annie Frazier, Maggie Hensley, Casey Hoffer, Miranda LaRue, Lauren Nyblade, Charlotte Parreño, Gladys Viviana Patton, John T. Shahrudin, Shabihah Yuan, Lijuan Zhou, Peng |
| author_facet | Nyblade, Charlotte Zhou, Peng Frazier, Maggie Frazier, Annie Hensley, Casey Fantasia-Davis, Ariana Shahrudin, Shabihah Hoffer, Miranda Agbemabiese, Chantal Ama LaRue, Lauren Barro, Mario Patton, John T. Parreño, Gladys Viviana Yuan, Lijuan |
| author_sort | Nyblade, Charlotte |
| collection | INTA Digital |
| description | Human rotavirus (HRV) is a leading cause of viral gastroenteritis in children across the globe. The virus has long been established as a pathogen of the gastrointestinal tract, targeting small intestine epithelial cells and leading to diarrhea, nausea, and vomiting. Recently, this classical infection pathway was challenged by the findings that murine strains of rotavirus can infect the salivary glands of pups and dams and transmit via saliva from pups to dams during suckling. Here, we aimed to determine if HRV was also capable of infecting salivary glands and spreading in saliva using a gnotobiotic (Gn) pig model of HRV infection and disease. Gn pigs were orally inoculated with various strains of HRV, and virus shedding was monitored for several days post-inoculation. HRV was shed nasally and in feces in all inoculated pigs. Infectious HRV was detected in the saliva of four piglets. Structural and non-structural HRV proteins, as well as the HRV genome, were detected in the intestinal and facial tissues of inoculated pigs. The pigs developed high IgM antibody responses in serum and small intestinal contents at 10 days post-inoculation. Additionally, inoculated pigs had HRV-specific IgM antibody-secreting cells present in the ileum, tonsils, and facial lymphoid tissues. Taken together, these findings indicate that HRV can replicate in salivary tissues and prime immune responses in both intestinal and facial lymphoid tissues of Gn pigs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15204 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA152042023-09-14T10:31:03Z Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs Nyblade, Charlotte Zhou, Peng Frazier, Maggie Frazier, Annie Hensley, Casey Fantasia-Davis, Ariana Shahrudin, Shabihah Hoffer, Miranda Agbemabiese, Chantal Ama LaRue, Lauren Barro, Mario Patton, John T. Parreño, Gladys Viviana Yuan, Lijuan Rotavirus Gnotobiotic Animals Swine Salivary Glands Immune Response Animales Notobióticos Cerdo Glándulas Salivales Respuesta Inmunológica Human Rotavirus Infection Infección por Rotavirus Humano Human rotavirus (HRV) is a leading cause of viral gastroenteritis in children across the globe. The virus has long been established as a pathogen of the gastrointestinal tract, targeting small intestine epithelial cells and leading to diarrhea, nausea, and vomiting. Recently, this classical infection pathway was challenged by the findings that murine strains of rotavirus can infect the salivary glands of pups and dams and transmit via saliva from pups to dams during suckling. Here, we aimed to determine if HRV was also capable of infecting salivary glands and spreading in saliva using a gnotobiotic (Gn) pig model of HRV infection and disease. Gn pigs were orally inoculated with various strains of HRV, and virus shedding was monitored for several days post-inoculation. HRV was shed nasally and in feces in all inoculated pigs. Infectious HRV was detected in the saliva of four piglets. Structural and non-structural HRV proteins, as well as the HRV genome, were detected in the intestinal and facial tissues of inoculated pigs. The pigs developed high IgM antibody responses in serum and small intestinal contents at 10 days post-inoculation. Additionally, inoculated pigs had HRV-specific IgM antibody-secreting cells present in the ileum, tonsils, and facial lymphoid tissues. Taken together, these findings indicate that HRV can replicate in salivary tissues and prime immune responses in both intestinal and facial lymphoid tissues of Gn pigs. Instituto de Virología Fil: Nyblade, Charlotte. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Zhou, Peng. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Frazier, Maggie. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Frazier, Annie. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Hensley, Casey. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Fantasia-Davis, Ariana. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Shahrudin, Shabihah. Indiana University. Department of Biology; Estados Unidos Fil: Hoffer, Miranda. Indiana University. Department of Biology; Estados Unidos Fil: Agbemabiese, Chantal Ama. Indiana University. Department of Biology; Estados Unidos Fil: LaRue, Lauren. GIVAX Inc.; Estados Unidos Fil: Barro, Mario. GIVAX Inc.; Estados Unidos Fil: Patton, John T. Indiana University. Department of Biology; Estados Unidos Fil: Parreño, Gladys Viviana. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Yuan, Lijuan. Virginia Polytechnic Institute and State University. Center for Emerging, Zoonotic, and Arthropod‑Borne Pathogens; Estados Unidos 2023-09-14T10:24:23Z 2023-09-14T10:24:23Z 2023-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15204 https://www.mdpi.com/1999-4915/15/9/1864 1999-4915 https://doi.org/10.3390/v15091864 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 15 (9) : 1864 (Agosto 2023) |
| spellingShingle | Rotavirus Gnotobiotic Animals Swine Salivary Glands Immune Response Animales Notobióticos Cerdo Glándulas Salivales Respuesta Inmunológica Human Rotavirus Infection Infección por Rotavirus Humano Nyblade, Charlotte Zhou, Peng Frazier, Maggie Frazier, Annie Hensley, Casey Fantasia-Davis, Ariana Shahrudin, Shabihah Hoffer, Miranda Agbemabiese, Chantal Ama LaRue, Lauren Barro, Mario Patton, John T. Parreño, Gladys Viviana Yuan, Lijuan Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| title | Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| title_full | Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| title_fullStr | Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| title_full_unstemmed | Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| title_short | Human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| title_sort | human rotavirus replicates in salivary glands and primes immune responses in facial and intestinal lymphoid tissues of gnotobiotic pigs |
| topic | Rotavirus Gnotobiotic Animals Swine Salivary Glands Immune Response Animales Notobióticos Cerdo Glándulas Salivales Respuesta Inmunológica Human Rotavirus Infection Infección por Rotavirus Humano |
| url | http://hdl.handle.net/20.500.12123/15204 https://www.mdpi.com/1999-4915/15/9/1864 https://doi.org/10.3390/v15091864 |
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