Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves
Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effe...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15203 https://www.mdpi.com/1999-4915/15/3/708 https://doi.org/10.3390/v15030708 |
| _version_ | 1855485516340789248 |
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| author | Bok, Marina Vega, Celina Guadalupe Castells, Matias Colina, Rodney Wigdorovitz, Andres Parreño, Gladys Viviana |
| author_browse | Bok, Marina Castells, Matias Colina, Rodney Parreño, Gladys Viviana Vega, Celina Guadalupe Wigdorovitz, Andres |
| author_facet | Bok, Marina Vega, Celina Guadalupe Castells, Matias Colina, Rodney Wigdorovitz, Andres Parreño, Gladys Viviana |
| author_sort | Bok, Marina |
| collection | INTA Digital |
| description | Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ELISA = 512; VN = 32) for 14 days as a passive treatment before a challenge with BCoV and were compared to calves fed milk with no supplementation. This is the first study with proof of efficacy of a product based on egg powder manufactured at a scale that successfully prevents BCoV-associated neonatal calf diarrhea. |
| format | Artículo |
| id | INTA15203 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA152032023-09-14T10:19:44Z Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves Bok, Marina Vega, Celina Guadalupe Castells, Matias Colina, Rodney Wigdorovitz, Andres Parreño, Gladys Viviana Diarrhoea COVID-19 Antibodies Development ELISA Bovine Viral Diarrhoea Dairy Cattle Diarrea Anticuerpos Desarrollo Diarrea Viral Bovina Ganado de Leche Bovine Coronavirus Diarrhea Dairy Calves Diarrea por Coronavirus Bovino Terneros Lecheros Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ELISA = 512; VN = 32) for 14 days as a passive treatment before a challenge with BCoV and were compared to calves fed milk with no supplementation. This is the first study with proof of efficacy of a product based on egg powder manufactured at a scale that successfully prevents BCoV-associated neonatal calf diarrhea. Instituto de Virología Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bok, Marina. Bioinnovo S.A.; Argentina Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina Fil: Vega, Celina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vega, Celina Guadalupe. Bioinnovo S.A.; Argentina Fil: Castells, Matias. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay Fil: Castells, Matias. National Institute of Agricultural Research (INIA). Animal Health Research; Uruguay Fil: Colina, Rodney. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay Fil: Colina, Rodney. National Institute of Agricultural Research (INIA). Animal Health Research; Uruguay Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT),; Argentina Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wigdorovitz, Andres. Bioinnovo S.A.; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Bioinnovo S.A.; Argentina 2023-09-14T10:11:42Z 2023-09-14T10:11:42Z 2023-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15203 https://www.mdpi.com/1999-4915/15/3/708 1999-4915 https://doi.org/10.3390/v15030708 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 15 (3) : 708 (Marzo 2023) |
| spellingShingle | Diarrhoea COVID-19 Antibodies Development ELISA Bovine Viral Diarrhoea Dairy Cattle Diarrea Anticuerpos Desarrollo Diarrea Viral Bovina Ganado de Leche Bovine Coronavirus Diarrhea Dairy Calves Diarrea por Coronavirus Bovino Terneros Lecheros Bok, Marina Vega, Celina Guadalupe Castells, Matias Colina, Rodney Wigdorovitz, Andres Parreño, Gladys Viviana Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves |
| title | Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves |
| title_full | Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves |
| title_fullStr | Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves |
| title_full_unstemmed | Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves |
| title_short | Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves |
| title_sort | development of an igy based treatment to control bovine coronavirus diarrhea in dairy calves |
| topic | Diarrhoea COVID-19 Antibodies Development ELISA Bovine Viral Diarrhoea Dairy Cattle Diarrea Anticuerpos Desarrollo Diarrea Viral Bovina Ganado de Leche Bovine Coronavirus Diarrhea Dairy Calves Diarrea por Coronavirus Bovino Terneros Lecheros |
| url | http://hdl.handle.net/20.500.12123/15203 https://www.mdpi.com/1999-4915/15/3/708 https://doi.org/10.3390/v15030708 |
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