Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows
The aim of this longitudinal study was to characterize the parasitemia of Neospora caninum and the associated immunological parameters in naturally infected beef cows for 10 months. The following groups were established: Neospora caninum seropositive pregnant cows (+Preg, n = 7), seropositive non-pr...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15195 https://www.frontiersin.org/articles/10.3389/fvets.2022.905271/full https://doi.org/10.3389/fvets.2022.905271 |
| _version_ | 1855037263810920448 |
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| author | Gual, Ignacio Campero, Lucia Maria Hecker, Yanina Regidor-Cerrillo, Javier Leunda, Maria Rosa Odeon, Anselmo Carlos Campero, Carlos Manuel Torioni, Susana Marta Echaide, Ignacio Eduardo Estein, Silvia Ortega Mora, Luis Miguel Moore, Prando Dadin |
| author_browse | Campero, Carlos Manuel Campero, Lucia Maria Echaide, Ignacio Eduardo Estein, Silvia Gual, Ignacio Hecker, Yanina Leunda, Maria Rosa Moore, Prando Dadin Odeon, Anselmo Carlos Ortega Mora, Luis Miguel Regidor-Cerrillo, Javier Torioni, Susana Marta |
| author_facet | Gual, Ignacio Campero, Lucia Maria Hecker, Yanina Regidor-Cerrillo, Javier Leunda, Maria Rosa Odeon, Anselmo Carlos Campero, Carlos Manuel Torioni, Susana Marta Echaide, Ignacio Eduardo Estein, Silvia Ortega Mora, Luis Miguel Moore, Prando Dadin |
| author_sort | Gual, Ignacio |
| collection | INTA Digital |
| description | The aim of this longitudinal study was to characterize the parasitemia of Neospora caninum and the associated immunological parameters in naturally infected beef cows for 10 months. The following groups were established: Neospora caninum seropositive pregnant cows (+Preg, n = 7), seropositive non-pregnant cows (+Npreg, n = 7), seronegative pregnant cows (−Preg, n = 4), and seronegative non-pregnant cows (−Npreg, n = 4). Several samples were obtained for absolute and relative leukocyte counting, cytokines IL-10, IL-12, α-TNF, and γ-IFN quantification, specific IgG, IgG1, and IgG2 and avidity and N. caninum DNA molecular detection and quantification. The +Preg group had a higher frequency and concentration of N. caninum DNA in PBMC in the last third of pregnancy compared to +Npreg (p < 0.05), with 22 and 8% of detection, respectively. Parasitemia correlated positively with IgG titers and negatively with IgG1/IgG2 ratio (p < 0.05). On day 222 of the assay, the +Preg group had the lowest total leukocyte counting (p < 0.05). The +Preg group had a higher concentration of IgG and higher avidity in the last third of gestation compared to +Npreg (p < 0.05). Avidity correlated with total IgG and IgG2 (p < 0.05). All +Preg cows gave birth to clinically healthy but seropositive calves before colostrum intake, therefore, the congenital transmission was 100% efficient. Only a complete N. caninum genotype from a placenta and a partial genotype from cow #3 of the group +Preg were achieved by multilocus microsatellite analysis. Overall, N. caninum parasitemia is frequent in seropositive beef cows during the last third of gestation. This correlates with higher antibody levels and a decrease in total leukocyte counting. The precise timing of the parasitemia may be used for diagnosis purposes and/or for design strategies to avoid vertical transmission. Further studies are needed to identify the immune molecular mechanisms that favor parasitemia during gestation in chronically infected cattle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15195 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA151952023-09-13T11:40:17Z Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows Gual, Ignacio Campero, Lucia Maria Hecker, Yanina Regidor-Cerrillo, Javier Leunda, Maria Rosa Odeon, Anselmo Carlos Campero, Carlos Manuel Torioni, Susana Marta Echaide, Ignacio Eduardo Estein, Silvia Ortega Mora, Luis Miguel Moore, Prando Dadin Fisiopatología Ganado Respuesta Inmunológica Parasitología Physiopathology Neospora caninum Livestock Immune Response Parasitology The aim of this longitudinal study was to characterize the parasitemia of Neospora caninum and the associated immunological parameters in naturally infected beef cows for 10 months. The following groups were established: Neospora caninum seropositive pregnant cows (+Preg, n = 7), seropositive non-pregnant cows (+Npreg, n = 7), seronegative pregnant cows (−Preg, n = 4), and seronegative non-pregnant cows (−Npreg, n = 4). Several samples were obtained for absolute and relative leukocyte counting, cytokines IL-10, IL-12, α-TNF, and γ-IFN quantification, specific IgG, IgG1, and IgG2 and avidity and N. caninum DNA molecular detection and quantification. The +Preg group had a higher frequency and concentration of N. caninum DNA in PBMC in the last third of pregnancy compared to +Npreg (p < 0.05), with 22 and 8% of detection, respectively. Parasitemia correlated positively with IgG titers and negatively with IgG1/IgG2 ratio (p < 0.05). On day 222 of the assay, the +Preg group had the lowest total leukocyte counting (p < 0.05). The +Preg group had a higher concentration of IgG and higher avidity in the last third of gestation compared to +Npreg (p < 0.05). Avidity correlated with total IgG and IgG2 (p < 0.05). All +Preg cows gave birth to clinically healthy but seropositive calves before colostrum intake, therefore, the congenital transmission was 100% efficient. Only a complete N. caninum genotype from a placenta and a partial genotype from cow #3 of the group +Preg were achieved by multilocus microsatellite analysis. Overall, N. caninum parasitemia is frequent in seropositive beef cows during the last third of gestation. This correlates with higher antibody levels and a decrease in total leukocyte counting. The precise timing of the parasitemia may be used for diagnosis purposes and/or for design strategies to avoid vertical transmission. Further studies are needed to identify the immune molecular mechanisms that favor parasitemia during gestation in chronically infected cattle. EEA Balcarce Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Leunda, María Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Torioni, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria Tandil; Argentina. Fil: Ortega Mora, Luis Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. 2023-09-13T11:31:52Z 2023-09-13T11:31:52Z 2022-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15195 https://www.frontiersin.org/articles/10.3389/fvets.2022.905271/full 22971769 https://doi.org/10.3389/fvets.2022.905271 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 9 : 905271 (June 2022) |
| spellingShingle | Fisiopatología Ganado Respuesta Inmunológica Parasitología Physiopathology Neospora caninum Livestock Immune Response Parasitology Gual, Ignacio Campero, Lucia Maria Hecker, Yanina Regidor-Cerrillo, Javier Leunda, Maria Rosa Odeon, Anselmo Carlos Campero, Carlos Manuel Torioni, Susana Marta Echaide, Ignacio Eduardo Estein, Silvia Ortega Mora, Luis Miguel Moore, Prando Dadin Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows |
| title | Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows |
| title_full | Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows |
| title_fullStr | Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows |
| title_full_unstemmed | Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows |
| title_short | Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows |
| title_sort | parasitemia and associated immune response in pregnant and non pregnant beef cows |
| topic | Fisiopatología Ganado Respuesta Inmunológica Parasitología Physiopathology Neospora caninum Livestock Immune Response Parasitology |
| url | http://hdl.handle.net/20.500.12123/15195 https://www.frontiersin.org/articles/10.3389/fvets.2022.905271/full https://doi.org/10.3389/fvets.2022.905271 |
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