Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15193 https://www.sciencedirect.com/science/article/pii/S0093691X22004009 https://doi.org/10.1016/j.theriogenology.2022.10.002 |
| _version_ | 1855037263447064576 |
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| author | Quintero Rodríguez, Luis Domínguez, Germán Alvarado Pinedo, María Travería, Gabriel Moré, Gastón Campero, Lucia Maria De la Sota, Luzbel Madoz, Laura Giuliodori, Mauricio |
| author_browse | Alvarado Pinedo, María Campero, Lucia Maria De la Sota, Luzbel Domínguez, Germán Giuliodori, Mauricio Madoz, Laura Moré, Gastón Quintero Rodríguez, Luis Travería, Gabriel |
| author_facet | Quintero Rodríguez, Luis Domínguez, Germán Alvarado Pinedo, María Travería, Gabriel Moré, Gastón Campero, Lucia Maria De la Sota, Luzbel Madoz, Laura Giuliodori, Mauricio |
| author_sort | Quintero Rodríguez, Luis |
| collection | INTA Digital |
| description | The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultrasound-guided technique for conceptus sampling. We run a prospective cohort study involving 92 cows (46 LEL and 46 NLEL). An LEL cow was that having an embryo with no heartbeat, detached membranes, or floating structures, including embryo remnants detected at pregnancy check by ultrasonography (US) 28–42 days post-AI, whereas an NLEL cow was that with embryo heartbeats detectable by US at pregnancy check 28–42 d post-IA. We took two blood samples from every cow at pregnancy check by US (the day of LEL detection) and 28 d later to perform serological diagnosis of BVDV, BoHV-1, and N. caninum; and to measure blood progesterone concentration at pregnancy check (28–42 d post-AI). We also sampled the conceptus from all the LEL cows. We performed PCR to detect BVDV, BoHV-1, and N. caninum in sampled conceptuses from LEL cows. Finally, we evaluated the associations of risk factors (serological titers, seroconversion, and progesterone) with LEL odds with logistic models. The risk for LEL was associated with serological titers to BVDV (P = 0.03) and tended to be associated with seroconversion to BVDV, given that 19.6% (9/46) in LEL and 6.5% (3/46) in NLEL cows seroconverted to BVDV (P = 0.09). In addition, BVDV was detected in conceptuses from LEL cows that seroconverted to BVDV but not in LEL cows that did not seroconvert. Conversely, the risk for LEL was not associated with the titers or seroconversion to BoHV-1 and N. caninum. BoHV-1 and N. caninum were not identified in any of the conceptuses. Finally, blood progesterone concentration was similar in LEL and NLEL cows, and it was not associated with the risk for LEL (P = 0.54). In conclusion, BVDV infection is a risk factor for LEL in dairy cows. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15193 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA151932023-09-13T11:10:26Z Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows Quintero Rodríguez, Luis Domínguez, Germán Alvarado Pinedo, María Travería, Gabriel Moré, Gastón Campero, Lucia Maria De la Sota, Luzbel Madoz, Laura Giuliodori, Mauricio Pestivirus de la Diarrea Bovina Herpes Virus Bovino Neospora caninum Progesterona Vacas Lecheras Bovine Diarrhoea Pestivirus Bovine Herpesvirus Progesterone Dairy Cows The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultrasound-guided technique for conceptus sampling. We run a prospective cohort study involving 92 cows (46 LEL and 46 NLEL). An LEL cow was that having an embryo with no heartbeat, detached membranes, or floating structures, including embryo remnants detected at pregnancy check by ultrasonography (US) 28–42 days post-AI, whereas an NLEL cow was that with embryo heartbeats detectable by US at pregnancy check 28–42 d post-IA. We took two blood samples from every cow at pregnancy check by US (the day of LEL detection) and 28 d later to perform serological diagnosis of BVDV, BoHV-1, and N. caninum; and to measure blood progesterone concentration at pregnancy check (28–42 d post-AI). We also sampled the conceptus from all the LEL cows. We performed PCR to detect BVDV, BoHV-1, and N. caninum in sampled conceptuses from LEL cows. Finally, we evaluated the associations of risk factors (serological titers, seroconversion, and progesterone) with LEL odds with logistic models. The risk for LEL was associated with serological titers to BVDV (P = 0.03) and tended to be associated with seroconversion to BVDV, given that 19.6% (9/46) in LEL and 6.5% (3/46) in NLEL cows seroconverted to BVDV (P = 0.09). In addition, BVDV was detected in conceptuses from LEL cows that seroconverted to BVDV but not in LEL cows that did not seroconvert. Conversely, the risk for LEL was not associated with the titers or seroconversion to BoHV-1 and N. caninum. BoHV-1 and N. caninum were not identified in any of the conceptuses. Finally, blood progesterone concentration was similar in LEL and NLEL cows, and it was not associated with the risk for LEL (P = 0.54). In conclusion, BVDV infection is a risk factor for LEL in dairy cows. EEA Balcarce Fil: Quintero Rodríguez, Luis. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: Quintero Rodríguez, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Domínguez, Germán. Actividad Privada; Argentina. Fil: Alvarado Pinedo, María. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: Moré, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: De la Sota. Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: De la Sota. Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Madoz, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. Fil: Madoz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Giuliodori, Mauricio. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina. 2023-09-13T11:05:26Z 2023-09-13T11:05:26Z 2022-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15193 https://www.sciencedirect.com/science/article/pii/S0093691X22004009 0093-691X (print) 1879-3231 (online) https://doi.org/10.1016/j.theriogenology.2022.10.002 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Theriogenology 194 : 126-132 (December 2022) |
| spellingShingle | Pestivirus de la Diarrea Bovina Herpes Virus Bovino Neospora caninum Progesterona Vacas Lecheras Bovine Diarrhoea Pestivirus Bovine Herpesvirus Progesterone Dairy Cows Quintero Rodríguez, Luis Domínguez, Germán Alvarado Pinedo, María Travería, Gabriel Moré, Gastón Campero, Lucia Maria De la Sota, Luzbel Madoz, Laura Giuliodori, Mauricio Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| title | Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| title_full | Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| title_fullStr | Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| title_full_unstemmed | Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| title_short | Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| title_sort | association of bovine viral diarrhea virus bovine herpesvirus 1 and neospora caninum with late embryonic losses in highly supplemented grazing dairy cows |
| topic | Pestivirus de la Diarrea Bovina Herpes Virus Bovino Neospora caninum Progesterona Vacas Lecheras Bovine Diarrhoea Pestivirus Bovine Herpesvirus Progesterone Dairy Cows |
| url | http://hdl.handle.net/20.500.12123/15193 https://www.sciencedirect.com/science/article/pii/S0093691X22004009 https://doi.org/10.1016/j.theriogenology.2022.10.002 |
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