A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental pur...
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| Format: | Artículo |
| Language: | Inglés |
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Les Amis d'Acarologia
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15173 https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4591 https://doi.org/10.24349/eyu1-eweg |
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| author | Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Landi, Lucas Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
| author_browse | Landi, Lucas Lanzavecchia, Silvia Beatriz Liendo, María Clara Muntaabski, Irina Russo, Romina Maria Scannapieco, Alejandra Carla |
| author_facet | Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Landi, Lucas Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
| author_sort | Muntaabski, Irina |
| collection | INTA Digital |
| description | Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments. |
| format | Artículo |
| id | INTA15173 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Les Amis d'Acarologia |
| publisherStr | Les Amis d'Acarologia |
| record_format | dspace |
| spelling | INTA151732024-07-12T16:24:35Z A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Landi, Lucas Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla Varroa destructor Apis mellifera Relaciones Huésped Parásito Ácaros Host Parasite Relations Mites Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments. Instituto de Investigación Genética Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; (CONICET) Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Genética; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina 2023-09-12T10:06:22Z 2023-09-12T10:06:22Z 2023-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15173 https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4591 2107-7207 https://doi.org/10.24349/eyu1-eweg eng info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001, DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVO info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Les Amis d'Acarologia Acarologia 63 (2) : 383-389 (Marzo 2023) |
| spellingShingle | Varroa destructor Apis mellifera Relaciones Huésped Parásito Ácaros Host Parasite Relations Mites Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Landi, Lucas Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle |
| title | A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle |
| title_full | A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle |
| title_fullStr | A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle |
| title_full_unstemmed | A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle |
| title_short | A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle |
| title_sort | method for semi field rearing of varroa destructor acari varroidae to obtain mites of controlled age and specific life cycle |
| topic | Varroa destructor Apis mellifera Relaciones Huésped Parásito Ácaros Host Parasite Relations Mites |
| url | http://hdl.handle.net/20.500.12123/15173 https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4591 https://doi.org/10.24349/eyu1-eweg |
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