Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance
Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Wiley
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15154 https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2018.12.0771 https://doi.org/10.2135/cropsci2018.12.0771 |
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| author | Brugnoli, Elsa Andrea Martinez, Eric Javier Ferrari Usandizaga, Silvana Consuelo Zili, Alex Leonel Urbani, Mario Acuña, Carlos Alberto |
| author_browse | Acuña, Carlos Alberto Brugnoli, Elsa Andrea Ferrari Usandizaga, Silvana Consuelo Martinez, Eric Javier Urbani, Mario Zili, Alex Leonel |
| author_facet | Brugnoli, Elsa Andrea Martinez, Eric Javier Ferrari Usandizaga, Silvana Consuelo Zili, Alex Leonel Urbani, Mario Acuña, Carlos Alberto |
| author_sort | Brugnoli, Elsa Andrea |
| collection | INTA Digital |
| description | Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids. |
| format | Artículo |
| id | INTA15154 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA151542023-09-08T14:39:07Z Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance Brugnoli, Elsa Andrea Martinez, Eric Javier Ferrari Usandizaga, Silvana Consuelo Zili, Alex Leonel Urbani, Mario Acuña, Carlos Alberto Gramíneas Mejoramiento Genético Híbridos Genotipos Apomixis Poliploidia Grasses Genetic Improvement Hybrids Genotypes Paspalum Polyploidy Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids. EEA Corrientes Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Martínez, Eric Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ferrari Usandizaga, Silvana Consuelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; Argentina Fil: Zili, Alex Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Zili, Alex Leonel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Urbani, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Urbani, Mario. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Acuña, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Acuña, Carlos Alberto. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina 2023-09-08T14:31:27Z 2023-09-08T14:31:27Z 2019 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15154 https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2018.12.0771 0011-183X https://doi.org/10.2135/cropsci2018.12.0771 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Crop Science 59 (4) : 1617-1624 (July-August 2019) |
| spellingShingle | Gramíneas Mejoramiento Genético Híbridos Genotipos Apomixis Poliploidia Grasses Genetic Improvement Hybrids Genotypes Paspalum Polyploidy Brugnoli, Elsa Andrea Martinez, Eric Javier Ferrari Usandizaga, Silvana Consuelo Zili, Alex Leonel Urbani, Mario Acuña, Carlos Alberto Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance |
| title | Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance |
| title_full | Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance |
| title_fullStr | Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance |
| title_full_unstemmed | Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance |
| title_short | Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts and Impact of Apomixis on Hybrid Performance |
| title_sort | breeding tetraploid paspalum simplex hybridization early identification of apomicts and impact of apomixis on hybrid performance |
| topic | Gramíneas Mejoramiento Genético Híbridos Genotipos Apomixis Poliploidia Grasses Genetic Improvement Hybrids Genotypes Paspalum Polyploidy |
| url | http://hdl.handle.net/20.500.12123/15154 https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2018.12.0771 https://doi.org/10.2135/cropsci2018.12.0771 |
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