Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination
A wide variety of plant-derived phytochemicals with anthelmintic effects have been described. Most of them have shown activity against parasites in vitro but have not been extensively explored in vivo. The aim of the current work was to study the pharmacokinetic–pharmacodynamic relationship of the c...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Multidisciplinary Digital Publishing Institute, MDPI
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15149 https://www.mdpi.com/2076-2615/13/8/1287 https://doi.org/10.3390/ani13081287 |
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| author | Miró, María Victoria Costa Júnior, Livio M. Lloberas, María Mercedes Cardozo, Patricia Alejandra Lanusse, Carlos Virkel, Guillermo Lifschitz, Adrián |
| author_browse | Cardozo, Patricia Alejandra Costa Júnior, Livio M. Lanusse, Carlos Lifschitz, Adrián Lloberas, María Mercedes Miró, María Victoria Virkel, Guillermo |
| author_facet | Miró, María Victoria Costa Júnior, Livio M. Lloberas, María Mercedes Cardozo, Patricia Alejandra Lanusse, Carlos Virkel, Guillermo Lifschitz, Adrián |
| author_sort | Miró, María Victoria |
| collection | INTA Digital |
| description | A wide variety of plant-derived phytochemicals with anthelmintic effects have been described. Most of them have shown activity against parasites in vitro but have not been extensively explored in vivo. The aim of the current work was to study the pharmacokinetic–pharmacodynamic relationship of the combined administration of carvone (R-CNE) and ivermectin (IVM) to lambs. Three trials were conducted to evaluate the pharmacological interaction between R-CNE and IVM in lambs infected with resistant nematodes. Drug concentrations were measured in plasma, target tissues, and H. contortus by HPLC with fluorescent (IVM) and ultraviolet (R-CNE) detection. The effect of both compounds on parasites was estimated by the fecal egg count reduction. Coadministration with R-CNE significantly increased the plasma bioavailability of IVM. R-CNE showed a moderate anthelmintic effect, which was greater on the susceptible isolate of H. contortus. After the combination of R-CNE and IVM as an oral emulsion, both compounds were quantified in H. contortus recovered from infected lambs. However, R-CNE concentrations were much lower than those reported to achieve anthelmintic effects in the in vitro assays. Optimization of the pharmaceutical formulation, dose rate, and administration schedule is needed to take advantage of the intrinsic anthelmintic activity of phytochemicals. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15149 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute, MDPI |
| publisherStr | Multidisciplinary Digital Publishing Institute, MDPI |
| record_format | dspace |
| spelling | INTA151492023-09-08T13:16:25Z Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination Miró, María Victoria Costa Júnior, Livio M. Lloberas, María Mercedes Cardozo, Patricia Alejandra Lanusse, Carlos Virkel, Guillermo Lifschitz, Adrián Nematodos Cordero Farmacocinética Farmacodinámica Nematodes Lambs Pharmacokinetics Pharmacodynamics Fitoquímicos A wide variety of plant-derived phytochemicals with anthelmintic effects have been described. Most of them have shown activity against parasites in vitro but have not been extensively explored in vivo. The aim of the current work was to study the pharmacokinetic–pharmacodynamic relationship of the combined administration of carvone (R-CNE) and ivermectin (IVM) to lambs. Three trials were conducted to evaluate the pharmacological interaction between R-CNE and IVM in lambs infected with resistant nematodes. Drug concentrations were measured in plasma, target tissues, and H. contortus by HPLC with fluorescent (IVM) and ultraviolet (R-CNE) detection. The effect of both compounds on parasites was estimated by the fecal egg count reduction. Coadministration with R-CNE significantly increased the plasma bioavailability of IVM. R-CNE showed a moderate anthelmintic effect, which was greater on the susceptible isolate of H. contortus. After the combination of R-CNE and IVM as an oral emulsion, both compounds were quantified in H. contortus recovered from infected lambs. However, R-CNE concentrations were much lower than those reported to achieve anthelmintic effects in the in vitro assays. Optimization of the pharmaceutical formulation, dose rate, and administration schedule is needed to take advantage of the intrinsic anthelmintic activity of phytochemicals. EEA Balcarce Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Costa Júnior, Livio M. Federal University of Maranhão. Center for Biological and Health Sciences; Brasil. Fil: Lloberas, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cardozo, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Lanusse, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Virkel, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Lifschitz, Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. 2023-09-08T13:12:18Z 2023-09-08T13:12:18Z 2023-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15149 https://www.mdpi.com/2076-2615/13/8/1287 2076-2615 https://doi.org/10.3390/ani13081287 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Multidisciplinary Digital Publishing Institute, MDPI Animals 13 (8) : 1287 (April 2023) |
| spellingShingle | Nematodos Cordero Farmacocinética Farmacodinámica Nematodes Lambs Pharmacokinetics Pharmacodynamics Fitoquímicos Miró, María Victoria Costa Júnior, Livio M. Lloberas, María Mercedes Cardozo, Patricia Alejandra Lanusse, Carlos Virkel, Guillermo Lifschitz, Adrián Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination |
| title | Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination |
| title_full | Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination |
| title_fullStr | Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination |
| title_full_unstemmed | Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination |
| title_short | Phytochemicals in Gastrointestinal Nematode Control: Pharmacokinetic–Pharmacodynamic Evaluation of the Ivermectin plus Carvone Combination |
| title_sort | phytochemicals in gastrointestinal nematode control pharmacokinetic pharmacodynamic evaluation of the ivermectin plus carvone combination |
| topic | Nematodos Cordero Farmacocinética Farmacodinámica Nematodes Lambs Pharmacokinetics Pharmacodynamics Fitoquímicos |
| url | http://hdl.handle.net/20.500.12123/15149 https://www.mdpi.com/2076-2615/13/8/1287 https://doi.org/10.3390/ani13081287 |
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