Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness
Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1514 http://www.sciencedirect.com/science/article/pii/S016816051630664X https://doi.org/10.1016/j.ijfoodmicro.2016.12.016 |
| _version_ | 1855482901341143040 |
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| author | Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria |
| author_browse | Alconada Magliano, Teresa Maria Bosch, Alejandra Fígoli, Cecilia Beatriz Gamba, Raúl Ricardo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Rojo, Rodrigo |
| author_facet | Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria |
| author_sort | Fígoli, Cecilia Beatriz |
| collection | INTA Digital |
| description | Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FTIR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore,
we could define several spectroscopic markers (NC\\H stretching modes in the 3000–2800 window, NC_O and\\C\\O vibrational modes of esters at 1765–1707 cm−1 and 1474–900 cm−1, respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis. |
| format | Artículo |
| id | INTA1514 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA15142022-08-12T16:18:30Z Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria Enfermedades de las Plantas Gibberella Zeae Trigo Fenotipos Espectroscopía Plant Diseases Wheat Phenotypes Spectroscopy Fusarium Graminearum Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 F. graminearum sensu stricto isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associated with aggressiveness. The combination of FTIR technology with hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (NC\\H stretching modes in the 3000–2800 window, NC_O and\\C\\O vibrational modes of esters at 1765–1707 cm−1 and 1474–900 cm−1, respectively), mostly assigned to lipid content that could be associated with F. graminearum aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of F. graminearum local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Fígoli, Cecilia Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina Fil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Kikot, Gisele Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina Fil: Leguizamón, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina Fil: Gamba, Raul Ricardo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Bosch, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Bioespectroscopía; Argentina Fil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina 2017-10-18T13:00:18Z 2017-10-18T13:00:18Z 2017-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1514 http://www.sciencedirect.com/science/article/pii/S016816051630664X 0168-1605 (Print) 1879-3460 (Online) https://doi.org/10.1016/j.ijfoodmicro.2016.12.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) International journal of food microbiology 244 : 36-42. (March 2017) |
| spellingShingle | Enfermedades de las Plantas Gibberella Zeae Trigo Fenotipos Espectroscopía Plant Diseases Wheat Phenotypes Spectroscopy Fusarium Graminearum Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Amelia Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, Alejandra Alconada Magliano, Teresa Maria Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness |
| title | Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness |
| title_full | Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness |
| title_fullStr | Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness |
| title_full_unstemmed | Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness |
| title_short | Characterization of Fusarium graminearum isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy : phenotypic diversity and detection of specific markers of aggressiveness |
| title_sort | characterization of fusarium graminearum isolates recovered from wheat samples from argentina by fourier transform infrared spectroscopy phenotypic diversity and detection of specific markers of aggressiveness |
| topic | Enfermedades de las Plantas Gibberella Zeae Trigo Fenotipos Espectroscopía Plant Diseases Wheat Phenotypes Spectroscopy Fusarium Graminearum |
| url | http://hdl.handle.net/20.500.12123/1514 http://www.sciencedirect.com/science/article/pii/S016816051630664X https://doi.org/10.1016/j.ijfoodmicro.2016.12.016 |
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