Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In t...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15098 https://www.mdpi.com/1999-4915/15/4/840 https://doi.org/10.3390/v15040840 |
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| author | Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas Esteban |
| author_browse | Bejerman, Nicolas Esteban Debat, Humberto Julio Garcia, Maria Laura |
| author_facet | Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas Esteban |
| author_sort | Debat, Humberto Julio |
| collection | INTA Digital |
| description | Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15098 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA150982023-09-05T10:24:59Z Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas Esteban Plant Viruses Virus de las Plantas Ophiovirus Virus Taxonomy Metatranscriptomics Virus Discovery Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. Instituto de Patología Vegetal Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Garcia, Maria Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2023-09-05T10:15:13Z 2023-09-05T10:15:13Z 2023-03-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15098 https://www.mdpi.com/1999-4915/15/4/840 1999-4915 (online) https://doi.org/10.3390/v15040840 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 15 (4) : 840 (2023) |
| spellingShingle | Plant Viruses Virus de las Plantas Ophiovirus Virus Taxonomy Metatranscriptomics Virus Discovery Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas Esteban Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_full | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_fullStr | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_full_unstemmed | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_short | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_sort | expanding the repertoire of the plant infecting ophioviruses through metatranscriptomics data |
| topic | Plant Viruses Virus de las Plantas Ophiovirus Virus Taxonomy Metatranscriptomics Virus Discovery |
| url | http://hdl.handle.net/20.500.12123/15098 https://www.mdpi.com/1999-4915/15/4/840 https://doi.org/10.3390/v15040840 |
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