Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior
Africanized Apis mellifera colonies with promising characteristics for beekeeping have been detected in northern Argentina (subtropical climate) and are considered of interest for breeding programs. Integral evaluation of this feral material revealed high colony strength and resistance/tolerance to...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Frontiers Media
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15070 https://www.frontiersin.org/articles/10.3389/finsc.2023.1175760/full https://doi.org/10.3389/finsc.2023.1175760 |
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| author | Bianchi, Eliana Mariel Ferrari, Carolina Aguirre, Natalia Cristina Filippi, Carla Valeria Vera, Pablo Alfredo Puebla, Andrea Fabiana Gennari, Gerardo Pablo Rodriguez, Graciela Adriana Scannapieco, Alejandra Carla Acuña, Cintia Vanesa Lanzavecchia, Silvia Beatriz |
| author_browse | Acuña, Cintia Vanesa Aguirre, Natalia Cristina Bianchi, Eliana Mariel Ferrari, Carolina Filippi, Carla Valeria Gennari, Gerardo Pablo Lanzavecchia, Silvia Beatriz Puebla, Andrea Fabiana Rodriguez, Graciela Adriana Scannapieco, Alejandra Carla Vera, Pablo Alfredo |
| author_facet | Bianchi, Eliana Mariel Ferrari, Carolina Aguirre, Natalia Cristina Filippi, Carla Valeria Vera, Pablo Alfredo Puebla, Andrea Fabiana Gennari, Gerardo Pablo Rodriguez, Graciela Adriana Scannapieco, Alejandra Carla Acuña, Cintia Vanesa Lanzavecchia, Silvia Beatriz |
| author_sort | Bianchi, Eliana Mariel |
| collection | INTA Digital |
| description | Africanized Apis mellifera colonies with promising characteristics for beekeeping have been detected in northern Argentina (subtropical climate) and are considered of interest for breeding programs. Integral evaluation of this feral material revealed high colony strength and resistance/tolerance to brood diseases. However, these Africanized honeybees (AHB) also showed variable negative behavioral traits for beekeeping, such as defensiveness, tendency to swarm and avoidance behavior. We developed a protocol for the selection of AHB stocks based on defensive behavior and characterized contrasting colonies for this trait using NGS technologies. For this purpose, population and behavioral parameters were surveyed throughout a beekeeping season in nine daughter colonies obtained from a mother colony (A1 mitochondrial haplotype) with valuable characteristics (tolerance to the mite Varroa destructor, high colony strength and low defensiveness). A Defensive Behavior Index was developed and tested in the colonies under study. Mother and two daughter colonies displaying contrasting defensive behavior were analyzed by ddRADseq. High-quality DNA samples were obtained from 16 workers of each colony. Six pooled samples, including two replicates of each of the three colonies, were processed. A total of 12,971 SNPs were detected against the reference genome of A. mellifera, 142 of which showed significant differences between colonies. We detected SNPs in coding regions, lncRNA, miRNA, rRNA, tRNA, among others. From the original data set, we also identified 647 SNPs located in protein-coding regions, 128 of which are related to 21 genes previously associated with defensive behavior, such as dop3 and dopR2, CaMKII and ADAR, obp9 and obp10, and members of the 5-HT family. We discuss the obtained results by considering the influence of polyandry and paternal lineages on the defensive behavior in AHB and provide baseline information to use this innovative molecular approach, ddRADseq, to assist in the selection and evaluation of honey bee stocks showing low defensive behavior for commercial uses. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15070 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA150702023-08-31T16:48:24Z Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior Bianchi, Eliana Mariel Ferrari, Carolina Aguirre, Natalia Cristina Filippi, Carla Valeria Vera, Pablo Alfredo Puebla, Andrea Fabiana Gennari, Gerardo Pablo Rodriguez, Graciela Adriana Scannapieco, Alejandra Carla Acuña, Cintia Vanesa Lanzavecchia, Silvia Beatriz Breeding Programmes High-throughput Sequencing Africanized Bees Programa de Crianza Secuenciación de Alto Rendimiento Apis Mellifera Abeja Africanizada Africanized Honey Bee Defensiveness Next Generation Sequencing Techniques ddRADseq Next Generation Sequencing Africanized Apis mellifera colonies with promising characteristics for beekeeping have been detected in northern Argentina (subtropical climate) and are considered of interest for breeding programs. Integral evaluation of this feral material revealed high colony strength and resistance/tolerance to brood diseases. However, these Africanized honeybees (AHB) also showed variable negative behavioral traits for beekeeping, such as defensiveness, tendency to swarm and avoidance behavior. We developed a protocol for the selection of AHB stocks based on defensive behavior and characterized contrasting colonies for this trait using NGS technologies. For this purpose, population and behavioral parameters were surveyed throughout a beekeeping season in nine daughter colonies obtained from a mother colony (A1 mitochondrial haplotype) with valuable characteristics (tolerance to the mite Varroa destructor, high colony strength and low defensiveness). A Defensive Behavior Index was developed and tested in the colonies under study. Mother and two daughter colonies displaying contrasting defensive behavior were analyzed by ddRADseq. High-quality DNA samples were obtained from 16 workers of each colony. Six pooled samples, including two replicates of each of the three colonies, were processed. A total of 12,971 SNPs were detected against the reference genome of A. mellifera, 142 of which showed significant differences between colonies. We detected SNPs in coding regions, lncRNA, miRNA, rRNA, tRNA, among others. From the original data set, we also identified 647 SNPs located in protein-coding regions, 128 of which are related to 21 genes previously associated with defensive behavior, such as dop3 and dopR2, CaMKII and ADAR, obp9 and obp10, and members of the 5-HT family. We discuss the obtained results by considering the influence of polyandry and paternal lineages on the defensive behavior in AHB and provide baseline information to use this innovative molecular approach, ddRADseq, to assist in the selection and evaluation of honey bee stocks showing low defensive behavior for commercial uses. Instituto de Investigación Animal del Chaco Semiárido Fil: Bianchi, Eliana Mariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área Animal; Argentina Fil: Ferrari, Carolina. Universidad Nacional del Noroeste de Buenos Aires (UNNOBA). Escuela de Ciencias Agrarias, Naturales y Ambientales (ECANA); Argentina Fil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Filippi, Carla Valeria. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica. Departamento de Biología Vegetal; Uruguay Fil: Vera, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vera, Pablo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Biotecnología. Unidad de Genómica; Argentina Fil: Puebla, Andrea Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Gennari, Gerardo Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Rodriguez, Graciela Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Ascasubi; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Acuña, Cintia Vanesa.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-08-31T16:37:38Z 2023-08-31T16:37:38Z 2023-08-31 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15070 https://www.frontiersin.org/articles/10.3389/finsc.2023.1175760/full 2673-8600 https://doi.org/10.3389/finsc.2023.1175760 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001, DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVO info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Insect Science 3 : 1175760 (31 August 2023) |
| spellingShingle | Breeding Programmes High-throughput Sequencing Africanized Bees Programa de Crianza Secuenciación de Alto Rendimiento Apis Mellifera Abeja Africanizada Africanized Honey Bee Defensiveness Next Generation Sequencing Techniques ddRADseq Next Generation Sequencing Bianchi, Eliana Mariel Ferrari, Carolina Aguirre, Natalia Cristina Filippi, Carla Valeria Vera, Pablo Alfredo Puebla, Andrea Fabiana Gennari, Gerardo Pablo Rodriguez, Graciela Adriana Scannapieco, Alejandra Carla Acuña, Cintia Vanesa Lanzavecchia, Silvia Beatriz Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior |
| title | Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior |
| title_full | Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior |
| title_fullStr | Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior |
| title_full_unstemmed | Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior |
| title_short | Phenotypic and genetic characterization of Africanized Apis mellifera colonies with natural tolerance to Varroa destructor and contrasting defensive behavior |
| title_sort | phenotypic and genetic characterization of africanized apis mellifera colonies with natural tolerance to varroa destructor and contrasting defensive behavior |
| topic | Breeding Programmes High-throughput Sequencing Africanized Bees Programa de Crianza Secuenciación de Alto Rendimiento Apis Mellifera Abeja Africanizada Africanized Honey Bee Defensiveness Next Generation Sequencing Techniques ddRADseq Next Generation Sequencing |
| url | http://hdl.handle.net/20.500.12123/15070 https://www.frontiersin.org/articles/10.3389/finsc.2023.1175760/full https://doi.org/10.3389/finsc.2023.1175760 |
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