Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination

Water-use efficiency (WUE) is a physiological parameter that plays a significant role in the evolutionary dynamics of many forest tree species. It can be estimated indirectly through carbon isotope discrimination (Δ). In general, plants of more arid origins have lower values of Δ. In order to study...

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Main Authors: Pastorino, Mario Juan, Aparicio, Alejandro Gabriel, Marchelli, Paula, Gallo, Leonardo Ariel
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), (España) 2023
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/15028
https://revistas.inia.es/index.php/fs/article/view/2272
https://doi.org/10.5424/fs/2012212-02272
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author Pastorino, Mario Juan
Aparicio, Alejandro Gabriel
Marchelli, Paula
Gallo, Leonardo Ariel
author_browse Aparicio, Alejandro Gabriel
Gallo, Leonardo Ariel
Marchelli, Paula
Pastorino, Mario Juan
author_facet Pastorino, Mario Juan
Aparicio, Alejandro Gabriel
Marchelli, Paula
Gallo, Leonardo Ariel
author_sort Pastorino, Mario Juan
collection INTA Digital
description Water-use efficiency (WUE) is a physiological parameter that plays a significant role in the evolutionary dynamics of many forest tree species. It can be estimated indirectly through carbon isotope discrimination (Δ). In general, plants of more arid origins have lower values of Δ. In order to study the degree of genetic control of this parameter and the genetic variation in Δ of Patagonian Cypress seedlings, three Argentinean natural populations chosen to represent two contrasting precipitation regimes were sampled in a common garden trial. The dry situation was represented by two neighboring marginal forest patches from the steppe, while the humid condition was represented by a population with 1,200 mm higher mean annual precipitation. Height (H) and Δ were measured in 246 five-year-old seedlings from 41 open-pollinated families. The factor ‘family’ had a significant effect on both variables; however heritability for Δ was found not to be significant in two out of the three populations. This could be explained by low sample size in one of them and by a real evolutionary effect in the other. An inverse association between H and Δ was verified, which is interpreted as evidence of an adaptation process at the intra-population level. The studied populations were not shown to discriminate carbon isotopes differently; hence evidence of adaptation to current environmental conditions could not be obtained. On the other hand, the arid populations proved to be quite different in terms of genetic variation, which seems to be the consequence of genetic drift and isolation.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2023
publishDateRange 2023
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publisher Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), (España)
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spelling INTA150282023-08-28T14:03:54Z Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination Pastorino, Mario Juan Aparicio, Alejandro Gabriel Marchelli, Paula Gallo, Leonardo Ariel Austrocedrus Deriva Genética Control Genético Régimen de las Precipitaciones Genetic Drift Genetic Control Precipitation Regime Patagonian Cypress Alerce Water-use efficiency (WUE) is a physiological parameter that plays a significant role in the evolutionary dynamics of many forest tree species. It can be estimated indirectly through carbon isotope discrimination (Δ). In general, plants of more arid origins have lower values of Δ. In order to study the degree of genetic control of this parameter and the genetic variation in Δ of Patagonian Cypress seedlings, three Argentinean natural populations chosen to represent two contrasting precipitation regimes were sampled in a common garden trial. The dry situation was represented by two neighboring marginal forest patches from the steppe, while the humid condition was represented by a population with 1,200 mm higher mean annual precipitation. Height (H) and Δ were measured in 246 five-year-old seedlings from 41 open-pollinated families. The factor ‘family’ had a significant effect on both variables; however heritability for Δ was found not to be significant in two out of the three populations. This could be explained by low sample size in one of them and by a real evolutionary effect in the other. An inverse association between H and Δ was verified, which is interpreted as evidence of an adaptation process at the intra-population level. The studied populations were not shown to discriminate carbon isotopes differently; hence evidence of adaptation to current environmental conditions could not be obtained. On the other hand, the arid populations proved to be quite different in terms of genetic variation, which seems to be the consequence of genetic drift and isolation. La eficiencia en el uso del agua es un parámetro fisiológico que desempeña un rol significativo en la dinámica evolutiva de muchas especies forestales. Puede estimarse indirectamente a través de la discriminación isotópica del carbono (Δ). En general, las plantas de orígenes más áridos tienen valores de Δ más bajos. Con el propósito de estudiar el grado de control genético de Δ y la variación genética en este parámetro en plántulas de Ciprés de la Cordillera, tres poblaciones naturales elegidas para representar dos regímenes de precipitación contrastados fueron muestreadas en un ensayo de ambiente común. La condición árida estuvo representada por dos fragmentos de bosque esteparios marginales, vecinos entre sí, mientras que la condición húmeda fue representada por una población con una precipitación media anual 1.200 mm superior a la de las áridas. Se midió altura total (H) y Δ en 246 plántulas de 5 años de edad correspondientes a 41 familias de polinización abierta. El factor ‘familia’ tuvo un efecto significativo en ambas variables; sin embargo, la heredabilidad para Δ no resultó significativa en dos de las tres poblaciones. En una de ellas esto podría explicarse por el restringido tamaño muestreal, mientras que en la otra por un verdadero efecto evolutivo. Asimismo se verificó una asociación inversa entre H y Δ, la cual es interpretada como evidencia de un proceso de adaptación a nivel intra-poblacional. No se observó que las poblaciones estudiadas discriminaran los isótopos del carbono de un modo diferencial, y por lo tanto no se obtuvieron evidencias de adaptación a las condiciones ambientales actuales. Por otro lado, las poblaciones áridas probaron ser muy diferentes entre sí en términos de variación genética, lo que parece ser la consecuencia de deriva y aislamiento genéticos. EEA Bariloche Fil: Pastorino, Mario Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina Fil: Pastorino, Mario Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina 2023-08-28T13:57:08Z 2023-08-28T13:57:08Z 2012-07-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15028 https://revistas.inia.es/index.php/fs/article/view/2272 2171-5068 2171-9845 https://doi.org/10.5424/fs/2012212-02272 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), (España) Forest Systems 21 (2) : 189-198. (Julio 2012)
spellingShingle Austrocedrus
Deriva Genética
Control Genético
Régimen de las Precipitaciones
Genetic Drift
Genetic Control
Precipitation Regime
Patagonian Cypress
Alerce
Pastorino, Mario Juan
Aparicio, Alejandro Gabriel
Marchelli, Paula
Gallo, Leonardo Ariel
Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
title Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
title_full Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
title_fullStr Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
title_full_unstemmed Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
title_short Genetic variation in seedling water-use efficiency of Patagonian Cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
title_sort genetic variation in seedling water use efficiency of patagonian cypress populations from contrasting precipitation regimes assessed through carbon isotope discrimination
topic Austrocedrus
Deriva Genética
Control Genético
Régimen de las Precipitaciones
Genetic Drift
Genetic Control
Precipitation Regime
Patagonian Cypress
Alerce
url http://hdl.handle.net/20.500.12123/15028
https://revistas.inia.es/index.php/fs/article/view/2272
https://doi.org/10.5424/fs/2012212-02272
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