Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
Lymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2023
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| Online Access: | http://hdl.handle.net/20.500.12123/15001 https://www.sciencedirect.com/science/article/pii/S2405939023000898 https://doi.org/10.1016/j.vprsr.2023.100919 |
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| author | Soler, Paula Abdala, Alejandra Mariana Larroza, Marcela Patricia |
| author_browse | Abdala, Alejandra Mariana Larroza, Marcela Patricia Soler, Paula |
| author_facet | Soler, Paula Abdala, Alejandra Mariana Larroza, Marcela Patricia |
| author_sort | Soler, Paula |
| collection | INTA Digital |
| description | Lymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can provide crucial information for designing strategic control programs. In this context, this work aimed at genetically characterizing the species of lymnaeid snails collected in different water bodies of northern Patagonia, Argentina. To this end, 689 snails were collected in 12 sites in the provinces of Neuquén, Río Negro and Chubut, in areas where fasciolosis is endemic. According to the morphological characteristics of their valves, they were identified as Galba spp. Twenty-three of these specimens were further identified using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2 and 18S rRNA. The results confirmed the identity of all the analyzed snails as Galba viatrix and provided evidence that studying the variable region V2 of the 18S rRNA gene is not enough to differentiate closely related species, as observed in lymnaeid snails. Both the fact that G. viatrix was the only species identified in the endemic area surveyed and previous evidence of the high prevalence of F. hepatica infestation in grazing animals in the region suggest that this species is the main intermediate host of F. hepatica. The correct identification of lymnaeid snail species has great importance to determine risk zones and develop appropriate control measures to reduce transmission, according to the different ecological characteristics of each species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15001 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA150012023-08-24T11:59:11Z Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina Soler, Paula Abdala, Alejandra Mariana Larroza, Marcela Patricia Caracoles Caracterización Molecular Distribución Geográfica Fasciola Hepatica Snails Molecular Characterization Geographical Distribution Región Patagónica Lymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can provide crucial information for designing strategic control programs. In this context, this work aimed at genetically characterizing the species of lymnaeid snails collected in different water bodies of northern Patagonia, Argentina. To this end, 689 snails were collected in 12 sites in the provinces of Neuquén, Río Negro and Chubut, in areas where fasciolosis is endemic. According to the morphological characteristics of their valves, they were identified as Galba spp. Twenty-three of these specimens were further identified using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2 and 18S rRNA. The results confirmed the identity of all the analyzed snails as Galba viatrix and provided evidence that studying the variable region V2 of the 18S rRNA gene is not enough to differentiate closely related species, as observed in lymnaeid snails. Both the fact that G. viatrix was the only species identified in the endemic area surveyed and previous evidence of the high prevalence of F. hepatica infestation in grazing animals in the region suggest that this species is the main intermediate host of F. hepatica. The correct identification of lymnaeid snail species has great importance to determine risk zones and develop appropriate control measures to reduce transmission, according to the different ecological characteristics of each species. EEA Bariloche Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Soler, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina 2023-08-24T11:50:56Z 2023-08-24T11:50:56Z 2023-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15001 https://www.sciencedirect.com/science/article/pii/S2405939023000898 2405-9390 https://doi.org/10.1016/j.vprsr.2023.100919 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Parasitology: Regional Studies and Reports 44 : 100919. (September 2023) |
| spellingShingle | Caracoles Caracterización Molecular Distribución Geográfica Fasciola Hepatica Snails Molecular Characterization Geographical Distribution Región Patagónica Soler, Paula Abdala, Alejandra Mariana Larroza, Marcela Patricia Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina |
| title | Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina |
| title_full | Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina |
| title_fullStr | Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina |
| title_full_unstemmed | Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina |
| title_short | Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina |
| title_sort | genetic characterization and regional distribution of lymnaeid snails in northern patagonia argentina |
| topic | Caracoles Caracterización Molecular Distribución Geográfica Fasciola Hepatica Snails Molecular Characterization Geographical Distribution Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/15001 https://www.sciencedirect.com/science/article/pii/S2405939023000898 https://doi.org/10.1016/j.vprsr.2023.100919 |
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