Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits
Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the pr...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1499 http://www.sciencedirect.com/science/article/pii/S1049964417300531 https://doi.org/10.1016/j.biocontrol.2017.03.005 |
| _version_ | 1855482898641059840 |
|---|---|
| author | Pesce, Virginia Mercedes Nally, María Cristina Carrizo, Gabriela P. Rojo, Maria Cecilia Perez, Beatriz Alida Toro, Maria Eugenia Castellanos de Figueroa, Lucía Inés Vazquez, Fabio |
| author_browse | Carrizo, Gabriela P. Castellanos de Figueroa, Lucía Inés Nally, María Cristina Perez, Beatriz Alida Pesce, Virginia Mercedes Rojo, Maria Cecilia Toro, Maria Eugenia Vazquez, Fabio |
| author_facet | Pesce, Virginia Mercedes Nally, María Cristina Carrizo, Gabriela P. Rojo, Maria Cecilia Perez, Beatriz Alida Toro, Maria Eugenia Castellanos de Figueroa, Lucía Inés Vazquez, Fabio |
| author_sort | Pesce, Virginia Mercedes |
| collection | INTA Digital |
| description | Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes |
| format | Artículo |
| id | INTA1499 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14992019-09-20T13:21:11Z Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits Pesce, Virginia Mercedes Nally, María Cristina Carrizo, Gabriela P. Rojo, Maria Cecilia Perez, Beatriz Alida Toro, Maria Eugenia Castellanos de Figueroa, Lucía Inés Vazquez, Fabio Enfermedades de las Plantas Colletotrichum Aceituna Levadura Control Biológico Plant Diseases Olives Yeasts Biological Control Colletotrichum Gloeosporioides Anthracnose is a fungal disease caused by Colletotrichum species, which mainly affects olive fruits during ripening. The use of yeasts from related and unrelated microenvironments in the control of plant pathogens has become an alternative to the use of synthetic fungicides. The objectives of the present study were to evaluate in vitro biocontrol strategies against C. gloeosporioides, in order to select native antagonistic yeasts isolated from different microenvironments, and to verify the in vivo biocontrol activity on ripe olive fruits. The potential human pathogenicity of the antagonistic yeasts was also considered. A total of 241 wild yeasts from diverse olive cultures and vitivinicultural microenvironments were assayed in vitro and in vivo for their antagonistic effect against the pathogen C. gloeosporioides. Ninety-two yeasts with in vitro characteristics of competitiveness such as niche overlap, dominance against the pathogen, reduction of fungal growth and production of antifungal metabolites were examined on ripe olive fruits. Nine biocontrol yeasts, three from fermenting grape must and six from olive fruits, leaves and branches in vivo reduced the incidence and severity of C. gloeosporioides. According to the 42 °C test, which assays for pseudohyphal formation and phospholipase production, these biocontrol yeasts did not present a health risk to humans. Microenvironments with selective stress factors can be relevant sources of microorganisms with biocontrol purposes Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina Fil: Carrizo, Gabriela P. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina Fil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez, Beatriz Alida. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina Fil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán; Argentina Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina 2017-10-17T15:25:49Z 2017-10-17T15:25:49Z 2017-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1499 http://www.sciencedirect.com/science/article/pii/S1049964417300531 1049-9644 https://doi.org/10.1016/j.biocontrol.2017.03.005 eng info:eu-repo/semantics/restrictedAccess application/pdf Biological control 120 : 43-51. (May 2018) |
| spellingShingle | Enfermedades de las Plantas Colletotrichum Aceituna Levadura Control Biológico Plant Diseases Olives Yeasts Biological Control Colletotrichum Gloeosporioides Pesce, Virginia Mercedes Nally, María Cristina Carrizo, Gabriela P. Rojo, Maria Cecilia Perez, Beatriz Alida Toro, Maria Eugenia Castellanos de Figueroa, Lucía Inés Vazquez, Fabio Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits |
| title | Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits |
| title_full | Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits |
| title_fullStr | Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits |
| title_full_unstemmed | Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits |
| title_short | Antifungal activity of native yeasts from different microenvironments against Colletotrichum gloeosporioides on ripe olive fruits |
| title_sort | antifungal activity of native yeasts from different microenvironments against colletotrichum gloeosporioides on ripe olive fruits |
| topic | Enfermedades de las Plantas Colletotrichum Aceituna Levadura Control Biológico Plant Diseases Olives Yeasts Biological Control Colletotrichum Gloeosporioides |
| url | http://hdl.handle.net/20.500.12123/1499 http://www.sciencedirect.com/science/article/pii/S1049964417300531 https://doi.org/10.1016/j.biocontrol.2017.03.005 |
| work_keys_str_mv | AT pescevirginiamercedes antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT nallymariacristina antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT carrizogabrielap antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT rojomariacecilia antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT perezbeatrizalida antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT toromariaeugenia antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT castellanosdefigueroaluciaines antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits AT vazquezfabio antifungalactivityofnativeyeastsfromdifferentmicroenvironmentsagainstcolletotrichumgloeosporioidesonripeolivefruits |